Sulfate-reducing bacteria metabolite detection using GC-MS

This research was conducted to investigate and compare the various metabolite, produced from the metabolism of two different SRB strain, that were involved in the biocorrosion process. Stainless steel coupons were immersed in two strains of sulfatereducing bacteria, A1H1 and EB3 (designated as SRB1 and SRB2 repectively) were isolated from Port Dickson marine vicinity that were grown in VMNI medium. The immersion period were set for eight days. Analysis of Gas Chromatography-Mass Spectrometry (GC-MS) was conducted using –trimethylsilyl (TMS) of N-methyl-N-trimethylsilylfluoracetamide (MSTFA). The data were than analyzed using Partial Least Squares Discriminant Analysis (PLS-DA) method to discriminate the unique metabolite according to each strain. The result showed that SRB1 generates less metabolite but high in concentration. Meanwhile, SRB2 shows a variety production of metabolites but less in concentration. Both strains share the same metabolism in the production of nitrogen based substance and production of norvaline and pentanoic acid. SRB1 shows a very distinct feature as the production of ribitol was spotted in its metabolism where it is usually associated with growth. SRB2 showed a very close usage of sulphur by production of methionine. These results suggest that different SRB strain produced different number and type of metabolites in the biocorrosion process

Comparison of biofilms morphology for two strains of marine sulfate-reducing bacteria using atomic force microscopy

AISI 304 stainless steel coupons were immersed in VMNI medium containing two strains of Sulfate-reducing bacteria which were isolated from Port Dickson, Negeri Sembilan. The immersion period were set for twenty-four hour, seven days, nine days and fifteen days. At the end of each period, the biofilm were formed on the surface will be maps using Atomic Force Microscopy. The image were taken using contact mode in the 70 μm x 70 μm and 10 μm x 10 μm resolution. Bacteria counts were done using the dilution and plate count technique. Image showed that SRB2 contain more bacteria cell attached to the metal surface by the 7th day of immersion compare to SRB1. But SRB1 produce more condensed and slime like biofilm with some crystallization precipitation while SRB2 bacteria cell were loosely attached. SRB1 bacteria cell were engulf in the slime like matrix while SRB2 bacteria cell is densely packed together. SRB2 showed a slightly higher rate growth in the medium compared to SRB1 but produce low amount of biofilm matrix while SRB1 produce a lot of bio-matrix precipitation and slime formation

Electrochemical impedance spectroscopy studies of stainless steel corrosion by two strains marine sulfate reducing bacteria

This research was conducted to compare corrosion potential of two strains of marine sulfate reducing bacteria (SRB1 and SRB2) on their effect of biocorrosion on stainless steel AISI304 using electrochemical techniques. This study was carried out using Electrochemical Impedance Spectroscopy (EIS) to determine the impedance value (passivity, Rp) and corrosion rate. An electrochemical measurement showed that the value of Impedance for Stainless steel in SRB culture is much smaller compare to VMNI medium (control), indicating a degradation of metal was occurred. SRB2 showed more aggressiveness on corrosion compare to SRB1 as the impedance value, Rp of stainless steel in SRB2 culture is lower compare to SRB1. In conclusion, EIS techniques given a good results to study mechanisms of corrosion and surface passivity