Magnetic fluid modified peanut husks as an adsorbent for organic dyes removal

AbstractMagnetically responsive nanocomposite materials, prepared by modification of diamagnetic materials by magnetic fluids (ferrofluids), have already found many important applications in various areas of biosciences, medicine, biotechnology, environmental technology etc. Ferrofluid modified biological waste (peanut husks) has been successfully used for the separation and removal of water soluble organic dyes and thus this low cost adsorbent could be potentially used for waste water treatment

Magnetic techniques for the isolation and purification of proteins and peptides

Isolation and separation of specific molecules is used in almost all areas of biosciences and biotechnology. Diverse procedures can be used to achieve this goal. Recently, increased attention has been paid to the development and application of magnetic separation techniques, which employ small magnetic particles. The purpose of this review paper is to summarize various methodologies, strategies and materials which can be used for the isolation and purification of target proteins and peptides with the help of magnetic field. An extensive list of realised purification procedures documents the efficiency of magnetic separation techniques

Microbial Reduction of Natural Fe(III) Minerals; Toward the Sustainable Production of Functional Magnetic Nanoparticles

The microbial synthesis of biominerals offers a potentially sustainable green solution for the production of a wide range of industrially relevant functional nanomaterials. Metal-reducing bacteria are of particular relevance, as they can enzymatically reduce a wide spectrum of high oxidation state metals and metalloids, forming cell-templated nanomagnets, catalysts, remediation agents, and quantum dots. Although these bioprocesses have been shown to be both scalable and tunable (with respect to particle size, reactivity, magnetic properties, and light emitting properties), they have yet to be taken up by industry. Here, we show that naturally abundant Fe(III) minerals are appropriate raw materials for the production of magnetic Fe(II)-bearing nanoparticles by the subsurface bacterium Geobacter sulfurreducens, and these bionanomaterials have the potential for remediation applications–here confirmed by the efficient reduction of toxic, mobile Cr(VI) to less toxic and soluble Cr(III). Detailed molecular-scale characterization of the bioreduced nanominerals, alongside life cycle assessments, and life cycle costings, confirm the efficient production of highly reactive and magnetic nanomaterials from waste materials. This adds further weight to the adoption of microbial technologies for sustainable, functional nanomaterials in a circular economy

Izolacija lizozima iz bjelanjka kokošjeg jajeta magnetskom kationskom izmjenom

Two magnetic macroporous cellulose cation exchangers (Iontosorb MG CM 100 and Iontosorb MG SHP 100) were used for one-step isolation of lysozyme from native, undiluted hen egg white. Highly purified lysozyme (purity >96 %) with specific activity similar to that of commercial lysozyme preparations was obtained in both cases. Carboxymethyl-based cation exchanger exhibited substantially higher capacity for lysozyme; maximum adsorption capacity was 138 mg/mL. The lysozyme-depleted egg white can be used in the same way as the routinely used egg white because no dilution of this material was necessary during the purification process.Dva magnetska kationska izmjenjivača od celuloznih vlakana velike poroznosti (Iontosorb MG CM 100 i Iontosorb MG SHP 100) upotrijebljena su da bi se jednostavno izolirao lizozim iz nerazrijeđenoga kokošjeg jajeta. U oba slučaja dobiven je lizozim velike čistoće (>96 %) čija je specifična aktivnost slična onoj komercijalnih lizozima. Kationski izmjenjivač na bazi karboksimetila imao je veću sposobnost adsorpcije lizozima (maksimalni kapacitet adsorpcije bio je 138 mg/mL). Nakon izdvajanja lizozima, bjelanjak se i dalje može upotrijebiti

MAGNETICALLY RESPONSIVE MATERIALS FOR SOLID PHASE EXTRACTION

Magnetically responsive materials have found many important applications in analytical chemistry. In this short review the basic information about Magnetic solid phase extraction and Magnetic textile solid phase extraction is given. These analytical techniques enable to preconcentrate target biologically active compounds or pollutants from water samples. Both procedures enable to lower the limit of detection using conventional analytical procedures

New magnetically responsive yeast-based biosorbent for the efficient removal of water-soluble dyes

Fodder yeast (Kluyveromyces fragilis) cells were magnetically modified by a contact with thewater-based magnetic fluid in order to prepare a new type of magnetically responsive biocomposite material. This procedure enabled a simple separation of modified cells by means of commercially available magnetic separators or strong permanent magnets. It allows using the prepared material as a new inexpensive magnetic affinity adsorbent for the removal of water-soluble dyes. Magnetically modified cells were characterized by means of magnetic and microscopy methods. Both isolated magnetic nanoparticles and aggregates of particles were present on the cell surface. The prepared material displayed a superparamagnetic behavior at room temperature, with a transition to a blocked state at TB 180K for the applied magnetic field H=50Oe. Seven dyes (crystal violet, amido black 10B, congo red, Saturn blue LBRR, Bismarck brown, acridine orange and safranin O) were used to study the adsorption process. The dyes adsorption could be described with the Langmuir isotherm. The maximum adsorption capacities ranged between 29.9 (amido black 10B) and 138.2 (safranin O) mg of dye per g of dried magnetically modified cells

Raising awareness, challenges, legislation, and mitigation approaches under the One Health concept

Funding also from project DigiAqua , grant ref. PTDCEEI-EEE/0415/2021 . M.F.C. and D.A.M.A. wish to acknowledge the funding from the project Ocean3R (NORTE-01-0145-FEDER-000064) , supported by the North Portugal Regional Operational Programme (NORTE2020), under the PORTUGAL2020 Partnership Agreement, and through the European Regional Development Fund (ERDF). L.L.B: the publication is part of a project that has received funding from the Erasmus + Project No. ECOBIAS_609967-EPP-1-2019-1-RS-EPPKA2-CBHE-JP ; GA.2019-1991/001-001 . Development of master curricula in ecological monitoring and aquatic bioassessment for Western Balkans HEIs/ECOBIAS. This work was also supported by the Ministry of Education, Science and Youth of Sarajevo Canton , grant ref. 27-02-11-4375-2/21 . This publication is based upon work from COST Action CA18238 (Ocean4Biotech, https://www.ocean4biotech.eu/ ), funded by the European Cooperation in Science and Technology (COST) Program , which provided open access support. Lada Lukić Bilela: Conceptualization, Formal analysis, Visualization, Writing – original draft, Writing – review & editing. Inga Matijošytė: Writing – original draft, Formal analysis, Visualization, Writing – review & editing. Jokūbas Krutkevičius: Writing – original draft, Visualization, Writing – review & editing. Diogo A. M. Alexandrino: Writing – original draft, Visualization, Writing – review & editing. Ivo Safarik: Writing – original draft, Visualization, Writing – review & editing. Juris Burlakovs: Writing – original draft, Writing – review & editing. Susana P. Gaudêncio: Conceptualization, Formal analysis, Visualization, Writing – original draft, Writing – review & editing. Maria F. Carvalho: Conceptualization, Formal analysis, Visualization, Writing – original draft, Writing – review & editing. All authors have read and agreed to the published version of the manuscript. Publisher Copyright: © 2023 The AuthorsPer- and polyfluorinated alkyl substances (PFAS) have long been known for their detrimental effects on the ecosystems and living organisms; however the long-term impact on the marine environment is still insufficiently recognized. Based on PFAS persistence and bioaccumulation in the complex marine food network, adverse effects will be exacerbated by global processes such as climate change and synergies with other pollutants, like microplastics. The range of fluorochemicals currently included in the PFAS umbrella has significantly expanded due to the updated OECD definition, raising new concerns about their poorly understood dynamics and negative effects on the ocean wildlife and human health. Mitigation challenges and approaches, including biodegradation and currently studied materials for PFAS environmental removal are proposed here, highlighting the importance of ongoing monitoring and bridging research gaps. The PFAS EU regulations, good practices and legal frameworks are discussed, with emphasis on recommendations for improving marine ecosystem management.publishersversionpublishe

Magnetically modified TiO2 powders - microstructure and magnetic properties

AbstractThe anatase (TiO2) particles magnetically modified by iron oxides and prepared by an innovating technological procedure are studied from the viewpoint of microstructure and a complex analysis of magnetic behaviour at room and elevated temperatures. Scanning electron microscopy observations have yielded variable shapes of particles in the composite powder whereas the iron oxide particles of diameter bellow 1μm were detected on the surface of the TiO2. The dominant magnetite (Fe3O4) accompanied by a small amount of maghemite (γ-Fe2O3) and/or hematite (α- Fe2O3) were analysed by X-ray powder diffraction. A relatively high saturation magnetization (3.38 Am2/kg), negative dipolar interactions, and the low values of reversible and irreversible part of magnetic susceptibility were found out from magnetic measurements at room temperature. During a thermomagnetic treatment the composite sample has been going through a few magnetic phase transitions and transforms into a fully paramagnetic state around 850K. After its cooling to the room temperature an undesirable magnetic hardening of the sample has occurred

Magnetic resonance investigation of magnetic-labeled baker's yeast cells

In this study, the interaction of DMSA-coated magnetite nanoparticles (5 and 10 nm core-size) with Saccharomyces cerevisae was investigated using magnetic resonance (MR) and transmission electron microscopy (TEM). The TEM micrographs revealed magnetite nanoparticles attached externally to the cell wall. The MR data support the strong interaction among the nanoparticles supported by the cells. A remarkable shift in the resonance field was used as signature of particle attachment to the cell wall