2 research outputs found
Integration of transcriptomics and proteomics Improves the characterization of the role of mussel gills in a bacterial waterborne infection
In recent years, the immune response of mussels (Mytilus galloprovincialis) has been studied at the transcriptomic level against several bacterial infections. As a result, different immune mechanisms have been revealed, including both conserved essential innate pathways and particularities of the mussel immune response according to its nature and environment. However, there is often a lack of functional verification because mussels are a non-model species and because transcriptomic and proteomic information is not always well correlated. In the current study, a high-throughput quantitative proteomics study coupled to LC-MS/MS analysis using isobaric tandem mass tags (TMTs) for protein labeling was employed to study the mussel gill immune response to a Vibrio splendidus bath (waterborne) infection at a functional protein level. A total of 4,242 proteins were identified and quantified, of which 226 were differentially expressed (DEPs) after infection, giving to the study a depth that was lacking in previous proteomic studies of the bivalve immune response. Modulated proteins evidenced an important cytoskeletal disruption caused by bacterial infection. A conserved network of associated proteins was modulated, regulating oxidative stress and NF-kB inflammatory responses and leading to innate immunity effectors. Proteomic results were submitted to an integrated analysis with those obtained in a previous transcriptomic approach with the same infection. Half of all the quantified proteins had a concordant transcriptomic expression trend, but this concordance increased when focusing on the DEPs. The correlation was higher within the immune-related DEPs, and the activation of the conserved NF-kB pro-inflammatory pathway was the main response in both approaches. The results of both techniques could be integrated to obtain a more complete vision of the response.Agencia Estatal de Investigaci贸n | Ref. RTI2018-095997-B-I00Xunta de Galicia | Ref. IN607B 2019/01Xunta de Galicia | Ref. ED431C 2020/05Agencia Estatal de Investigaci贸n | Ref. PRE2019-09076
A resposta inmune do mexill贸n Mytilus galloprovincialis. Transcript贸mica, prote贸mica e aspectos evolutivos
The project will focus on the analysis of the innate immune system of the Mytilus galloprovincialis mussel and the genetic variability of the gene families responsible for antimicrobial recognition and the triggering of the immune response. The immune response of mussels to a bacterial infection will be studied at the transcriptomic and proteomic level. The key gene families involved in the response will be studied from an evolutionary perspective to understand the particularities of the mussel immune system.El proyecto consistir谩 en analizar el sistema inmune innato del mejill贸n Mytilus galloprovincialis y la variabilidad gen茅tica de las familias de genes responsables del reconocimiento antimicrobiano y el desencadenamiento de la respuesta inmune. Se estudiar谩 la respuesta del mejill贸n a una infecci贸n bacteriana a nivel transcript贸mico y prote贸mico. Las familias de genes clave involucradas en la respuesta se estudiar谩n desde una perspectiva evolutiva para comprender las particularidades del sistema inmune del mejill贸n.O proxecto consistir谩 en analizar o sistema inmune innato do mexill贸n Mytilus galloprovincialis e a variabilidade xen茅tica das familias x茅nicas responsables do reco帽ecemento antimicrobiano e do desencademanto da resposta inmune. A resposta do mexill贸n ante unha infecci贸n bacteriana ser谩 estudada a nivel transcript贸mico e prote贸mico. As familias x茅nicas clave implicadas na resposta ser谩n estudadas dende un prisma evolutivo para entender as particularidades do sistema inmune do mexill贸n.Agencia Estatal de Investigaci贸n | Ref. RTI2018-095997-B-I00Ministerio de Econom铆a y Competitividad | Ref. AGL2015-65705-RAgencia Estatal de Investigaci贸n | Ref. PID2021-124955OB-I0