Synthesis, In- vivo and In-silico anti-inflammatory studies of substituted fluoro pyrazole

N-phenyl-5-substituted -aryl-3-p-(fluorophenyl) pyrazoles have been synthesized from cyclization of 4-fluoroacetophenone (1) with various benzaldehydes (2) to give 4-fluorophenylstyrylketone (3) followed by treatment with phenyl hydrazine. The title compounds and their derivatives have been characterized by their elemental and spectral analysis. The newly synthesized compounds are screened for anti-inflammatory activity. All substituted 4-fluorophenylstyrylketones (250mg/kg orally p.o.) possessed anti-inflammatory activity against carrageenan-induced paw oedema in rat. Indomethacin (10mg/kg) was used as standard drug. And all compounds (0.20mM) showed ability to denature bovine serum albumin as observed in vitro inhibition studies. No correlation was found between the anti inflammatory activity and inhibition of bovine serum albumin denaturation. The docking studies were carried out for these compounds against the protein NFκB which is involved in inflammation signal cascade. Some of them showed good activity and molecular binding. Compounds such as 3b, 5b and 5e have exhibited comparative results in both in vivo and in silico studies