Structural and Molecular Characterization of Squalene Synthase Belonging to the Marine Thraustochytrid Species Aurantiochytrium limacinum Using Bioinformatics Approach

The marine microorganisms thraustochytrids have been explored for their potential in the production of various bioactive compounds, such as DHA, carotenoids, and squalene. Squalene is a secondary metabolite of the triterpenoid class and is known for its importance in various industrial applications. The bioinformatic analysis for squalene synthase (SQS) gene (the first key enzyme in the triterpenoid synthesis pathway), that is prevailing among thraustochytrids, is poorly investigated. In-silico studies combining sequence alignments and bioinformatic tools helped in the preliminary characterization of squalene synthases found in Aurantiochytrium limacinum. The sequence contained highly conserved regions for SQS found among different species indicated the enzyme had all the regions for its functionality. The signal peptide sequence and transmembrane regions were absent, indicating an important aspect of the subcellular localization. Secondary and 3-D models generated using appropriate templates demonstrated the similarities with SQS of the other species. The 3-D model also provided important insights into possible active, binding, phosphorylation, and glycosylation sites

Dydrogesterone use in recurrent pregnancy loss and luteal phase insufficiency: a knowledge, attitude, and practice survey amongst Indian obstetricians and gynaecologists

Background: Oral dydrogesterone is effective in treating reproductive disorders such as recurrent pregnancy loss (RPL) and is a better option than progesterone for luteal phase support (LPS) after in-vitro fertilization (IVF). This study aimed to assess the attitude, knowledge, and practice of obstetricians and gynecologists (ObGyns) across India towards the usage of dydrogesterone in RPL and LPS in real-life setting. Methods: A validated questionnaire-based survey was conducted among Indian ObGyns and results were expressed in percentages. Results: 393 ObGyns participated in the survey. 61.3% of ObGyns agreed RPL is the three consecutive pregnancy losses prior to 20 weeks from the last menstrual period. With the use of dydrogesterone in RPL patients, 82.6% of ObGyns found ˃40% of patients having successful pregnancy outcomes. 37.2% of ObGyns mentioned that dydrogesterone is useful in ongoing pregnancy rates while 33% reported benefits in the live birth rate. At the risk for preterm delivery before 34 gestational weeks, 57% of ObGyns see benefits of dydrogesterone. More than 95% of ObGyns observed benefits of dydrogesterone over micronized progesterone such as good efficacy, fewer side effects, better tolerability, and improved patient compliance. 51.9% of ObGyns reported that they used dydrogesterone in combination with progesterone in LPS. Conclusions: The present KAP survey highlights the observation, perception, and practicing behaviour of ObGyns on RPL and LPS. It also highlights the usage of dydrogesterone in aforesaid indications

Structural and Molecular Characterization of Squalene Synthase Belonging to the Marine Thraustochytrid Species Aurantiochytrium limacinum Using Bioinformatics Approach

The marine microorganisms thraustochytrids have been explored for their potential in the production of various bioactive compounds, such as DHA, carotenoids, and squalene. Squalene is a secondary metabolite of the triterpenoid class and is known for its importance in various industrial applications. The bioinformatic analysis for squalene synthase (SQS) gene (the first key enzyme in the tri-terpenoid synthesis pathway), that is prevailing among thraustochytrids, is poorly investigated. In-silico studies combining sequence alignments and bioinformatic tools helped in the preliminary characterization of squalene synthases found in Aurantiochytrium limacinum. The sequence contained highly conserved regions for SQS found among different species indicated the enzyme had all the regions for its functionality. The signal peptide sequence and transmembrane regions were absent, indicating an important aspect of the subcellular localization. Secondary and 3-D models generated using appropriate templates demonstrated the similarities with SQS of the other species. The 3-D model also provided important insights into possible active, binding, phosphorylation, and glycosylation sites

ONLINE SHOPPING AND FOLKLORE - A REVIEW: WITH SPECIAL REFERENCE TO INDIA

The purpose of this research paper is to classify factors affecting the online shopping in India. The weighty growth of online shopping is noticeable in India. More and more consumer is transferring to online shopping rather than traditional buying methods. Many myths and misconceptions emerge in India regarding online shopping. Every new thing, when it is not cleared by all the people properly, it is only a nightmare. Online shopping is intricate in nature. As we know the number of online transactions have been increased, but it is still under the dark room. Government and E-commerce companies should take necessary steps for reducing the level of misconceptions and myths of online shopping in India. This research paper’s purpose is to identifying ‘How’ the companies can be able to overcome these fake information regarding online shopping. Also improve company’s sales figure, for the upliftment of company as well as an Indian economy.</jats:p