The Regenerative Effect of Intra-Articular Injection of Autologous Fat Micro-Graft in Treatment of Chronic Knee Osteoarthritis

The study started in 2010 to find the effect of autologous fat micrograft for osteoarthritis (OA); the result was published on normal animal’s model, in 10 patients, then in 80 patients with knee osteoarthritis, and the current study in 205 patients. The study was conducted at King Abdulaziz University Hospital (January 2012–October 2015); 80 adult patients were suffering from moderate to severe knee osteoarthritis. About 10–20 mL fat micrograft was prepared with liposuction and injected intra-articularly into the affected knee/s. The results revealed that pain improvement after the fat injection during rest and with activity with the visual analogue scale. The Western Ontario and McMaster Universities Osteoarthritis Index (WOMAC) indicated improvement, both in the three domains (pain, stiffness, and physical function) and in total. The use of intra-articular autologous fat micrograft is simple, safe, and effective for degenerative knee osteoarthritis

مادة فعّالة (ب إم 701) تهاجم الخلايا السرطانية (ل 1210) في الفئران المتسرطنة : دراسة إكلينيكية و دراسة نسيجية مرضية

Abstract. Cancer therapy remains a challenging problem. PM 701 is an ideal anti-cancer agent, which selectively targets cancer cells, thereby sparing normal tissues. In the first stage of this study, PM 701 induced programmed cell death (apoptosis) on cancer cells without any side effects on normal cells at tissue culture level. The aim of this study was to test the effect of PM 701 agent on cancer-induced animal models which were successfully produced at our laboratory. L1210 leukemia cells were inoculated intraperitoneally in adult male MFI mice which were divided into 4 groups. Group One – injected intraperitoneally with leukemia cells without treatment; Group Two – injected with L1210 cells and treated with oral administration of (PM 701) at the end of the 1st week; Group Three – received PM 701 only, and Group Four – control mice that drank only distilled water. The animal model was successfully produced when the inoculated tumor cells were grown locally and produced distant metastases. This was confirmed by the growth of peritoneal mass, positive blood smear and histopathological study which revealed systemic infiltration of leukemia cells in the liver, lung, kidney, spleen and testes. The use of PM 701 in cancer induced animals indicated its efficacy which showed marked improvement of the animals ' clinical condition, improved animal survival rate, negative blood smear; decrease in the size of pertioneal mass, reduction of metastatic deposits in some organs and eradication in others

Intra-articular Injection of Autologous Fat Micro Graft in Sheep Hind Knee Joints

Abstract: Background: The aim of this study is to evaluate the safety and effects of intra-articular injection of Autologous fat micro graft into normal animals hind knee joints. Methods: Six sheep were enrolled in the study. Under monitored anesthesia care, autologous fat micro graft was harvested from tail fat of the animal using tumescent technique. 7-10 ml of the separated fat was injected intra-articularly to the Rt hind knee joint of the animal leaving the Lt one as control for comparison. Clinical observation of the animals in using their fat injected joints was recorded over the 12 weeks of the experiment.histological examinations of the fat injected and the control joints were done at the end of the experiment. Results: All the animals involved in the study were using their treated joints normally on the day following the injection till the end of the experiment. 12 weeks after fat injection the macroscopic and microscopic examinations of both treated and control femoral and tibial surfaces of articular cartilage of hind knee joints showed no sign of joints pathological changes, however, the chondrocytes of femoral and tibial articular cartilage in treated limbs showed proliferation including deferent mitotic stages. Conclusion: the injection of autologous fat micro graft intra-articularlyis safe practice with no clinical negative manifestations in relation to the fat injected joints of the experimental animals, the macroscopic and microscopic study of the injected joints did not show any pathological changes but give indications of positive proliferative effect on chondrocytesof cartilage in the treated joint