Sub-milliKelvin spatial thermometry of a single Doppler cooled ion in a Paul trap

We report on observations of thermal motion of a single, Doppler-cooled ion along the axis of a linear radio-frequency quadrupole trap. We show that for a harmonic potential the thermal occupation of energy levels leads to Gaussian distribution of the ion's axial position. The dependence of the spatial thermal spread on the trap potential is used for precise calibration of our imaging system's point spread function and sub-milliKelvin thermometry. We employ this technique to investigate the laser detuning dependence of the Doppler temperature.Comment: 5 pages, 4 figure

Insect resistance of a full sib family of tetraploid switchgrass \u3ci\u3ePanicum virgatum\u3c/i\u3e L. with varying lignin levels

Little information is available on insect resistance mechanisms and inheritance in biomass grasses. Although reduction of lignin in biomass grasses in order to increase the efficiency of fermentation may result in increased susceptibility to insect feeding, other resistance mechanisms may be more important. Field grown leaves of two tetraploid parent (Kanlow N1, Summer) and 14 progeny switchgrass (Panicum virgatum L.) plant clones selected for a diversity of plant form and ranges in lignin levels were tested for leaf resistance to feeding by the fall armyworm (Spodoptera frugiperda J. E. Smith), a grass feeding insect pest. Although lignin generally appeared important as a resistance mechanism only at early season stages, replicate clones of two low lignin progeny plants generally remained resistant to fall armyworm feeding. Mechanical damaging increased resistance to fall armyworm feeding in several of these plants. Degrees of resistance were sometimes associated with leaf form of progeny. These results indicate there are likely multiple insect resistance mechanisms operating at different stages in switchgrass, and that segregation of some mechanisms appears related to growth form of the plants

Formation of 3-methyl-1,2,3-butanetricarboxylic acid via gas phase oxidation of pinonic acid : a mass spectrometric study of SOA aging

This paper presents the results of mass spectrometric investigations of the OH-initiated oxidative aging of α-pinene SOA under simulated tropospheric conditions at the large aerosol chamber facility AIDA, Karlsruhe Institute of Technology. In particular, the OH-initiated oxidation of pure pinic and pinonic acid, two well-known oxidation products of α-pinene, was investigated. Two complementary analytical techniques were used, on-line atmospheric pressure chemical ionization/mass spectrometry (APCI/MS) and filter sampling followed by liquid chromatography/mass spectrometry (LC/ESI-MS). The results show that 3-methyl-1,2,3-butanetricarboxylic acid (MBTCA), a very low volatile α-pinene SOA product and a tracer compound for terpene SOA, is formed from the oxidation of pinonic acid and that this oxidation takes place in the gas phase. This finding is confirmed by temperature-dependent aging experiments on whole SOA formed from α-pinene, in which the yield of MBTCA scales with the pinonic acid fraction in the gas phase. Based on the results, several feasible gas-phase radical mechanisms are discussed to explain the formation of MBTCA from OH-initiated pinonic acid oxidation

In non-transformed cells Bak activates upon loss of anti-apoptotic Bcl-X-L and Mcl-1 but in the absence of active BH3-only proteins

Mitochondrial apoptosis is controlled by proteins of the B-cell lymphoma 2 (Bcl-2) family. Pro-apoptotic members of this family, known as BH3-only proteins, initiate activation of the effectors Bcl-2-associated X protein (Bax) and Bcl-2 homologous antagonist/killer (Bak),which is counteracted by anti-apoptotic family members. How the interactions of Bcl-2 proteins regulate cell death is still not entirely clear. Here, we show that in the absence of extrinsic apoptotic stimuli Bak activates without detectable contribution from BH3-only proteins, and cell survival depends on anti-apoptotic Bcl-2 molecules. All anti-apoptotic Bcl-2 proteins were targeted via RNA interference alone or in combinations of two in primary human fibroblasts. Simultaneous targeting of B-cell lymphoma-extra large and myeloid cell leukemia sequence 1 led to apoptosis in several cell types. Apoptosis depended on Bak whereas Bax was dispensable. Activator BH3-only proteins were not required for apoptosis induction as apoptosis was unaltered in the absence of all BH3-only proteins known to activate Bax or Bak directly, Bcl-2-interacting mediator of cell death, BH3-interacting domain death agonist and p53-upregulated modulator of apoptosis. These findings argue for auto-activation of Bak in the absence of anti-apoptotic Bcl-2 proteins and provide evidence of profound differences in the activation of Bax and Bak

In non-transformed cells Bak activates upon loss of anti-apoptotic Bcl-X-L and Mcl-1 but in the absence of active BH3-only proteins

Mitochondrial apoptosis is controlled by proteins of the B-cell lymphoma 2 (Bcl-2) family. Pro-apoptotic members of this family, known as BH3-only proteins, initiate activation of the effectors Bcl-2-associated X protein (Bax) and Bcl-2 homologous antagonist/killer (Bak),which is counteracted by anti-apoptotic family members. How the interactions of Bcl-2 proteins regulate cell death is still not entirely clear. Here, we show that in the absence of extrinsic apoptotic stimuli Bak activates without detectable contribution from BH3-only proteins, and cell survival depends on anti-apoptotic Bcl-2 molecules. All anti-apoptotic Bcl-2 proteins were targeted via RNA interference alone or in combinations of two in primary human fibroblasts. Simultaneous targeting of B-cell lymphoma-extra large and myeloid cell leukemia sequence 1 led to apoptosis in several cell types. Apoptosis depended on Bak whereas Bax was dispensable. Activator BH3-only proteins were not required for apoptosis induction as apoptosis was unaltered in the absence of all BH3-only proteins known to activate Bax or Bak directly, Bcl-2-interacting mediator of cell death, BH3-interacting domain death agonist and p53-upregulated modulator of apoptosis. These findings argue for auto-activation of Bak in the absence of anti-apoptotic Bcl-2 proteins and provide evidence of profound differences in the activation of Bax and Bak

Beam pattern evolution of accreting X-ray pulsar 1A 0535+262 during its 2020 giant outburst

We report on pulse profile decomposition analysis of a bright transient X-ray pulsar 1A 0535+262 using the broadband Insight-HXMT observations during a giant outburst of the source in 2020. We show that the observed pulse profile shape can be described in terms of a combination of two symmetric single-pole contributions for wide range of energies and luminosities for a fixed geometry defining basic geometry of the pulsar. This corresponds to a slightly distorted dipole magnetic field, i.e., one pole has to be offset by $\sim 12^{\circ}$ from the antipodal position of the other pole. We reconstruct the intrinsic beam patterns of the pulsar assuming the geometry recovered from the decomposition analysis, and find evidence for a transition between "pencil" and "fan" beams in energy ranges above the cyclotron line energy which can be interpreted as transition from sub- to super-critical accretion regimes associated with onset of an accretion column. At lower energies the beam pattern appears, however, to be more complex, and contains substantial "fan" beam and an additional "pencil" beam component at all luminosities. The latter is not related to the accretion rate and is stronger in the fading phase of the outburst. We finally discuss results in context of other observational and theoretical findings earlier reported for the source in the literature.Comment: 14 pages, 8 figures. Accepted by Ap

Sources of organic gases and aerosol particles and their roles in nighttime particle growth at a rural forested site in southwest Germany

The composition, sources and chemical transformation of volatile organic compounds (VOCs) and organic aerosol (OA) particles were investigated during July–August 2021 at a rural forested site in southwest Germany, 10 km north of the city of Karlsruhe. VOCs and semi-volatile OA particles were measured with a proton-transfer-reaction mass spectrometer coupled to the CHemical Analysis of aeRosols ONline particle inlet (CHARON–PTR-MS). The CHARON-measured OA mass accounted for 62 ± 18 % on average of the total OA mass (4.2 ± 2.8 µg m−3) measured concurrently with an aerosol mass spectrometer (AMS). The total concentrations of measured VOCs ranged from 7.6 to 88.9 ppb with an average of 31.2 ± 13.4 ppb. Positive matrix factorization (PMF) was used to identify major source factors of VOCs and OA particles. Three types of oxygenated VOC (OVOC), namely aromatic OVOCs, biogenic OVOCs and aged OVOCs, contributed on average 11 % ± 9 %, 37 % ± 29 % and 29 % ± 21 % of total VOC concentrations, respectively. The results of AMS–PMF indicated substantial contributions of oxygenated organic compounds to OA particle mass. Three secondary OA (SOA) factors determined by CHARON–PMF analysis, namely aromatic SOA (5 % ± 7 %), daytime biogenic SOA (17 % ± 17 %) and nighttime biogenic SOA (28 % ± 21 %), consistently showed high contributions to the total CHARON-measured OA mass. Nighttime particle growth was observed regularly in this area, which was mainly attributed to the semi-volatile organic compounds and organic nitrates formed from the oxidation of monoterpenes and sesquiterpenes. This study presents the major sources, real-time transformations of VOCs and OA particles, and nighttime particle formation characteristics for central European forested areas.</p

Comparison of scanning aerosol lidar and in situ measurements of aerosol physical properties and boundary layer heights

The spatiotemporal distribution of aerosol particles in the atmosphere has a great impact on radiative transfer, clouds, and air quality. Modern remote sensing methods, as well as airborne in situ measurements by unpiloted aerial vehicles (UAV) or balloons, are suitable tools to improve our understanding of the role of aerosol particles in the atmosphere. To validate the measurement capabilities of three relatively new measurement systems and to bridge the gaps that are often encountered between remote sensing and in situ observation, as well as to investigate aerosol particles in and above the boundary layer, we conducted two measurement campaigns and collected a comprehensive dataset employing a scanning aerosol lidar, a balloon-borne radiosonde with the Compact Optical Backscatter Aerosol Detector (COBALD), an optical particle counter (OPC) on a UAV, and a comprehensive set of ground-based instruments. The extinction coefficients calculated from near-ground-level aerosol size distributions measured in situ are well correlated with those retrieved from lidar measurements, with a slope of 1.037 ± 0.015 and a Pearson correlation coefficient of 0.878, respectively. Vertical profiles measured by an OPC-N3 on a UAV show similar vertical particle distributions and boundary layer heights to lidar measurements. However, the sensor, OPC-N3, shows a larger variability in the aerosol backscatter coefficient measurements, with a Pearson correlation coefficient of only 0.241. In contrast, the COBALD data from a balloon flight are well correlated with lidar-derived backscatter data from the near-ground level up to the stratosphere, with a slope of 1.063 ± 0.016 and a Pearson correlation coefficient of 0.925, respectively. This consistency between lidar and COBALD data reflects the good data quality of both methods and proves that lidar can provide reliable and spatial distributions of aerosol particles with high spatial and temporal resolutions. This study shows that the scanning lidar has the capability to retrieve backscatter coefficients near the ground level (from 25 to 50 m above ground level) when it conducts horizontal measurement, which is not possible for vertically pointing lidar. These near-ground-level retrievals compare well with ground-level in situ measurements. In addition, in situ measurements on the balloon and UAV validated the scanning lidar retrievals within and above the boundary layer. The scanning aerosol lidar allows us to measure aerosol particle distributions and profiles from the ground level to the stratosphere with an accuracy equal to or better than in situ measurements and with a similar spatial resolution.</p

Selective scattering between Floquet-Bloch and Volkov states in a topological insulator

The coherent optical manipulation of solids is emerging as a promising way to engineer novel quantum states of matter. The strong time periodic potential of intense laser light can be used to generate hybrid photon-electron states. Interaction of light with Bloch states leads to Floquet-Bloch states which are essential in realizing new photo-induced quantum phases. Similarly, dressing of free electron states near the surface of a solid generates Volkov states which are used to study non-linear optics in atoms and semiconductors. The interaction of these two dynamic states with each other remains an open experimental problem. Here we use Time and Angle Resolved Photoemission Spectroscopy (Tr-ARPES) to selectively study the transition between these two states on the surface of the topological insulator Bi2Se3. We find that the coupling between the two strongly depends on the electron momentum, providing a route to enhance or inhibit it. Moreover, by controlling the light polarization we can negate Volkov states in order to generate pure Floquet-Bloch states. This work establishes a systematic path for the coherent manipulation of solids via light-matter interaction.Comment: 21 pages, 6 figures, final version to appear in Nature Physic