Uncommon Location of Lipoma: Extraluminally Vagina

Background: Lipomas are defined as a soft mass of well-differentiated adipose cells among mesenchymal tumors. Considering the localization of lipomas, these masses are commonly seen in subcutaneous tissue. The female reproductive tract is a rare site for development of lipomas and clinical findings appear depending on the size and localization. The connection of the mass with the vagina lumen causes different clinical findings. Intraluminal tumors protrude and arise from the vulva, while extraluminal tumors lead to perineal swelling. This case describes clinical, ultrasonographic, histological examination, and surgical management of extraluminal vaginal lipoma.Case: The present study reports a case of extraluminal vaginal lipoma in a 10-year-old Pitbull crossbred presented with anamnesis of a protruded tissue from the vulvar lips and dysuria following 6-month swelling in the perineum. Clinical examination revealed that the swelling was obvious and localized mostly on the lower left side of the perineum and the vaginal mucosa was protruded from the vulvar lips due to a mass. By vaginal palpation, the mass was not associated with the vaginal mucosa. The mass was located in the perivaginal region and transvaginal ultrasonography revealed a hypoechogenic mass. Based on clinical and ultrasonographic findings, surgery was recommended. The mass was adherent to the serosa of the vagina and it did not enclose the vaginal mucosa. Excision of mass was performed with careful blunt dissection avoiding any urethral disruption and periurethral tissues. After the mass was determined to be extraluminal, there was no need for excision of the vaginal tissue with the mass during operation. Using histopathological examination the mass was identified as a lipoma composed of adipocytes. In addition, ovariohysterectomy was not recommended following the diagnosis of vaginal lipoma.Discussion: Lipomas uncommonly can be localized in unpredicted rare areas such as vagina and vulva in older bitches. Although the majority of reported benign tumors in dogs recommend the surgery, medical treatment including the steroid injections is the other option to limit the lipomas. Considering the vaginal tumors, the surgical approach is also planned as laparotomy since leiomyomas are more common than lipomas. This approach partially results from the adherence of tumors to vaginal tissue. In addition, the location of the mass together without the adjacent tissue damage depending on whether it is intraluminal or extraluminal would make complete tissue removal. In previous reports, while symptoms such as perineal hernia of the lipoma were emphasized, the connection of the lipoma with the vaginal lumen was not evaluated as a significant factor determining the surgical approach. The mass was not connected to the vaginal mucosa and it was determined as extraluminal lipoma in the present study. Mass excision was performed by blunt dissection, avoiding urethral disruption and periurethral tissues. Unlike the most common approach in leiomyomas, this report did not require a total vaginectomy to remove the mass from the vagina. The ovariohysterectomy was not needed to be performed in the present study as in intraluminal vaginal tumors. It was believed that adhesions of lipoma with vaginal lumen is a determining factor in the combination of surgical techniques such as episiotomy and/or ovariohysterectomy. Lipomas should be considered among the tumors in the vaginal region of dogs brought with the complaint of swelling in perineum and protrusion of vaginal tissue

Effects of Different Estrous Synchronization Methods on the Composition of Simmental Cow’s Milk

Background: Estrous synchronization is one of the primary applications performed to improve the fertility and to consolidate the parturition times in dairy cattle farms. Since the primary objective is to increase fertility regardless of the method of synchronization used, the effect of this process on the udder health and the quality of milk is generally ignored. Therefore, in the present study, the aim was to investigate the effect of different estrous synchronization methods t on the milk quality in a dairy cattle farm.Materials, Methods & Results: In this study, a total of 100 Simmental cows the same farm under good management practices were used. The animals were randomly divided into 4 groups of 25 cows each. The animals in the 1st group were used as a control group without any treatment. Progesterone-releasing intravaginal device (PRID) was inserted to the animals in the 2nd group, double dose of prostaglandin F2 alpha (PGF2-alpha) with 11 days apart was applied to the animals in the 3rd group, and the Ovsynch protocol was applied to the animals in the 4th group. Starting from the first day of the applications, milk samples from each milk tank belonging to the groups were taken, kept frozen for 3 months and analyzed for the levels of fat, nonfat solids, density, protein, lactose and minerals over the course of one month. According to the results, the minimum milk fat content was found in the PRID group as 2.47 ± 0.354%, and the highest value was in the PGF2-alpha group as 3.58 ± 0.207%. The difference in milk fat ratio between the groups was found to be significant (P < .05). However, the differences between the groups for other parameters were not significant (P > .05).Discussion: Among the reasons for the low values of the milk fat rates obtained in the present study than the mean values of all lactation milk fat rates of the Simmental breed, the effect of the lactation period in which the milk samples were collected comes to mind. Because, as we know, the first 45-60 day period following the start of lactation after parturition in cattle is accepted as the period in which daily milk yield reaches to the maximum level of the lactation period. This period lasts for a certain period of time, and then starts to decrease gradually. Among the non-hereditary factors, the most important factor causing changes in the composition of the milk and its amount is the lactation period. In this study, the significant difference in the milk fat rates between the control and the application groups is quite remarkable compared to the low level of milk fat rates in the milk in all groups. According to the multiple comparison test results to determine which groups these differences arise from, the difference was found to be particularly between the PGF2-alpha group and PRID group. In this study, no significant effects of different synchronization methods on all components of the milk except the milk fat and the density values were determined. But results indicate that Ovsynch group was the synchronization group that caused the minimum change in milk fat compared to the other groups

Somatic cell count and bacteriological evaluation of milk obtained from clinically healthy goat

Bu çalışmada keçi sütü örneklerinin somatik hücre sayısı yönünden analizi, aerobik bakterin izolasyonu, izole edilen S. aureus suşlarının çeşitli antibiyotiklere in vitro duyarlılıklarının saptanması ve metisilin dirençli S. aureus suşlarının ortaya konulması amaçlandı. Toplam 313 süt örneğinin ortalama somatik hücre sayısı 3,25x103, subklinik mastitisli 214 (%68,3) süt örneğinin 1,9x106, S. aureus izole edilen 32 (%27,6) süt örneğinin ise 1x106 hücre/ml olarak belirlendi. Kültür yöntemiyle sütlerin 116 (%54,2) adetinden bakteri izole edildi. Örneklerin 46’sından (%39,6) koagulaz negatif stafilokoklar, 32’sinden S. aureus (%27,6) izole edildi. Toplam 7 farklı antibiyotiğin test edildiği bu çalışmada, 32 adet S. aureus suşundan 27’si (%84,3) penisilin G’ye, 17’si (%53,1) amoksisilin/klavulonik asit ve sefaprezone, 15’i (%46,8) marbofloksasine, 13’ü (%40,6) enrofloksasine, 9’u (%28,1) seftiofura ve 8’i (%25) ise gentamisine dirençli bulundu. S. aureus suşlarından 8’inin (%25,0) metisilin dirençli S. aureus ve 5’i (%62,5), çoklu antibiyotik dirence sahipti. Sonuç olarak, i) ortalama somatik hücre sayısının düşük olduğu ii) S. aureus suşlarının penisilin G, amoksisilin/klavulonik asit ve sefaperozone yüksek düzeyde dirençli olduğu, iii) metisilin dirençli S. aureus oranının %25 olduğu görüldü.In this study, it was aimed to analyze goat milk samples in terms of somatic cell number, isolation of aerobic bacteria, to determine the in vitro susceptibility of isolated S. aureus strains to various antibiotics and to reveal methicillin resistant S. aureus strains. The mean number of somatic cells of 313 milk samples examined was 3.25x103, 1.9x106 for 214 (68.3%) milk samples with subclinical mastitis, and 1x106 cells/mL for 32 (27.6%) milk samples with S. aureus isolated. Bacteria were isolated from 116 (54.2%) milk samples by culture method. Coagulase negative staphylococci from 46 (39.6%) and S. aureus from 32 (27.6%) isolated. Of the 32 S. aureus strains tested, 27 (84.3%) were resistant to penicillin G, 17 (53.1%) to amoxicillin/clavulanic acid and cefaprezone, 15 (46.9%) to marbofloxacin, 13 (34.3%) to enrofloxacin, 9 (28.1%) to ceftiofur and 8 (25.0%) to gentamicin. Eight of the S. aureus strains (25.0%) had methicillin-resistant S. aureus and 5 (62.5%) had multi-antibiotic resistance. As a result, it was determined that i) the mean somatic cell count was low, ii) S. aureus strains were highly resistant to penicillin G, amoxicillin/clavulanic acid and cefaperozone, iii) methicillin-resistant S. aureus was found to be 25% of the rate

Türkiye'de farklı niteliklere sahip süt sığırı işletmelerinde mastitisin koruma ve kontrolü kapsamındaki bazı uygulamaların düzeyleri

Amaç: Sunulan çalışmada Türkiye’deki 177 küçük ve 169 büyük ölçeklisüt sığırı işletmesinde mastitisin koruma ve kontrolu?ne yönelik uygulamalar ve uygulanma düzeylerinin ortaya koyulması amaçlandı.Gereç ve Yöntem: Bu kapsamda Türkiye’de farklı illerdeki toplam 346süt sığırı işletmesinden meme sağlığı ile ilgili verileri toplanarak istatistiksel açıdan incelendi.Bulgular: Büyük ölçekli işletmelerde en fazla 50-200 baş (n=96, %56,8ve en fazla Holştayn ırkı (n=97, %57,4)) ve küçük ölçekli işletmelerde iseen fazla 11-20 baş (n=71, %40,1 ve en fazla Simental ırkı (n=153, %86,4))arası inek olduğu, bu iki tip işletmede 4 yaşlı ineklerin daha fazla olduğu(sırasıyla, n=62, %36,7 ve n=93, %52,5) görülmüştür. Büyük işletmelerdeteat dipping (%70,4), kuru dönem antibiyotik (%72,8), teat seal(%9,5) vemastitis aşısı (%55,6) uygulamalarının küçük ölçekli işletmelerden dahayüksek olduğu tespit edilmiştir. Büyük işletmelerde predipping (%59,2)ve postdipping uygulaması yapanların oranının (%67,5) yapmayanlardandaha fazla olduğu ve uygulamalarda en fazla iyotlu antiseptiklerin kullanıldığı belirlenmiştir. Küçük ölçekli işletmelerde sağım hijyeni uygulamayanların oranının (%61,6) fazla olduğu tespit edilmiştir. Bu iki tip işletmede günlük ortalama süt verimi üzerine işletme tipi (p<0,000) ve ırkın(p<0,001) etkisi istatistiksel olarak önemli bulunmuştur.Öneri: Sonuç olarak, Türkiye’deki süt sığırı işletmelerinde mastitisdenkoruma ve mastitisin kontrolu? amaçlı uygulanan yöntemlerin oranlarının,geçmiş yıllarla karşılaştırıldığında daha yüksek olduğu fakat istenen düzeylerde olmadığı tespit edilmiştir

Effects of Different Estrous Synchronization Methods on the Composition of Simmental Cow’s Milk

Background: Estrous synchronization is one of the primary applications performed to improve the fertility and to consolidate the parturition times in dairy cattle farms. Since the primary objective is to increase fertility regardless of the method of synchronization used, the effect of this process on the udder health and the quality of milk is generally ignored. Therefore, in the present study, the aim was to investigate the effect of different estrous synchronization methods t on the milk quality in a dairy cattle farm.Materials, Methods &amp; Results: In this study, a total of 100 Simmental cows the same farm under good management practices were used. The animals were randomly divided into 4 groups of 25 cows each. The animals in the 1st group were used as a control group without any treatment. Progesterone-releasing intravaginal device (PRID) was inserted to the animals in the 2nd group, double dose of prostaglandin F2 alpha (PGF2-alpha) with 11 days apart was applied to the animals in the 3rd group, and the Ovsynch protocol was applied to the animals in the 4th group. Starting from the first day of the applications, milk samples from each milk tank belonging to the groups were taken, kept frozen for 3 months and analyzed for the levels of fat, nonfat solids, density, protein, lactose and minerals over the course of one month. According to the results, the minimum milk fat content was found in the PRID group as 2.47 ± 0.354%, and the highest value was in the PGF2-alpha group as 3.58 ± 0.207%. The difference in milk fat ratio between the groups was found to be significant (P &lt; .05). However, the differences between the groups for other parameters were not significant (P &gt; .05).Discussion: Among the reasons for the low values of the milk fat rates obtained in the present study than the mean values of all lactation milk fat rates of the Simmental breed, the effect of the lactation period in which the milk samples were collected comes to mind. Because, as we know, the first 45-60 day period following the start of lactation after parturition in cattle is accepted as the period in which daily milk yield reaches to the maximum level of the lactation period. This period lasts for a certain period of time, and then starts to decrease gradually. Among the non-hereditary factors, the most important factor causing changes in the composition of the milk and its amount is the lactation period. In this study, the significant difference in the milk fat rates between the control and the application groups is quite remarkable compared to the low level of milk fat rates in the milk in all groups. According to the multiple comparison test results to determine which groups these differences arise from, the difference was found to be particularly between the PGF2-alpha group and PRID group. In this study, no significant effects of different synchronization methods on all components of the milk except the milk fat and the density values were determined. But results indicate that Ovsynch group was the synchronization group that caused the minimum change in milk fat compared to the other groups

Absence of link between abortion and seropositivity of cystic hydatid disease in ewes and female goats in Turkey

This study was conducted to test whether there is a statistically significant association between seropositivity to cystic echinococcosis and abortion in ewes and female goats from the Turkish provinces of Elazig (east Anatolia), Sanliurfa (south-east Anatolia) and Kayseri (Inner Anatolia) using the enzyme-linked immunosorbent assay (ELISA). A total of 20 of 133 sera (15.1%) from ewes and 5 of 101 sera (4.9%) from goats with a history of abortion gave seropositive results that were not significantly different (p>0.05) from these, 9.9% (10/101) were reported for ewes and 1.6% (2/122) for female goats without a history of abortion. Serological prevalence rates among those animals with a history of abortion were not significantly different from the control group. No positive association was established between seropositivity for cystic echinococcosis and abortion in ewes and female goats

Effect of melatonin on torsion and reperfusion induced pathogenesis of rat uterus

We investigated the use of melatonin to improve fertility and reduce uterine damage caused by torsion of the uterus in pregnant rats. We used 35 pregnant rats at gestational age 18 days. The animals were randomized into five groups. Group 1 was anesthetized only. Group 2 was subjected to experimental uterine torsion of 360 degrees and the torsion was corrected after 6 h. Group 3 was subjected to uterine torsion of 360 degrees, the torsion was corrected after 6 h and melatonin was administered at the time of correction. Group 4 rats were subjected to 360o uterine torsion and melatonin was administered 6 h later at the time of correction. Group 5 was administered melatonin followed by uterine torsion of 360 degrees followed by correction of torsion 6 h later. Samples were obtained from the uterine horns on the day 1 postpartum. We used Bax, Bcl-2 and caspase 3 staining to measure apoptosis in the uterine tissues. The mRNA levels of Rho-associated, coiled-coil containing protein kinases 1 (ROCK1), homeobox D10 (Hox4 HoxD10), TLR4, NF kappa B1, caveolin 1 (Cav1) heat shock protein 90 alpha (cytosolic), class B member 1 (Hsp90ab1) and glyceraldehyde-3-phosphate dehydrogenase (GAPDH) were determined using quantitative real-time polymerase chain reaction analysis (qRT-PCR). Bax, Bcl-2 and caspase 3 were detected using immunohistochemistry. No difference was observed among groups with respect to abortion, neonatal mortality or congenital abnormalities. Compared to the control group, the mRNA levels of Rock1, Hox4, TLR4, NF kappa B1, Cav1 and Hsp90 genes were decreased significantly in the study groups; the decrease was greater in groups 3 and 4, which were treated with melatonin. The greatest amount of Bax staining was found in group 1 and the least amount of Bcl-2 staining was found in groups 4 and 5; the greatest amount of caspase 3 staining was found in group 2. Our findings indicate that melatonin reduced uterine torsion related tissue damage and that its application during torsion was more effective than application following removal of torsion.Scientific and Technological Research Council of TurkeyTurkiye Bilimsel ve Teknolojik Arastirma Kurumu (TUBITAK) [TUBITAK - 115O381]; TUBITAKTurkiye Bilimsel ve Teknolojik Arastirma Kurumu (TUBITAK) [115O381]Our study was supported by the Scientific and Technological Research Council of Turkey (TUBITAK - 115O381); TUBITAK [115O381]

The effect of melatonin in rats with uterine torsion on uterus contractions, and the levels of ADMA, SDMA, arginine, Hsp90, TLR4, and NF-kappa B

In this study was aimed at reducing uterine damage and increasing fertility after uterine torsion in pregnant animals. With this aim, uterine torsion was experimentally formed in 35 rats that were between 18-19 days pregnant. The animals were randomly divided into five groups, and melatonin was administered prior to torsion, at the time of torsion, and detorsion (10 mg/kg/gun IP). Ovario-hysterectomy operation was performed on all animals on the first day following parturition. Subsequently, from the obtained uterus samples, determination of the levels of asymmetrical dimethyl arginin (ADMA), symmetrical dimethyl arginin (SDMA), and arginine was made using the high-performance liquid chromatography (HPLC) and levels of Heat shock protein 90 (Hsp90), TLR4 (Toll Like Receptor 4) and NF kappa B (Nuclear factor kappa-light-chain-enhancer of activated B cells) were measured using the Western blot technique. The contraction-relaxation responses of the myometrium were also determined in the organ baths. According to the results of the western blot, higher protein expressions than those of the control group were determined in the second, third, fourth, and fifth groups in Hsp90, TLR4, NF-kappa B. The lowest values of arginine and ADMA were found in Group 3, whilst the lowest SDMA value was determined in Group 1. It was determined that melatonin reduces tissue damage secondary to torsio uteri and, furthermore, that administration of this hormone at the time of torsion formation was more effective than its administration at the time of detorsion.Scientific and Technological Research Council of TurkeyTurkiye Bilimsel ve Teknolojik Arastirma Kurumu (TUBITAK) [TUBITAK - 115O381]This study was supported by the Scientific and Technological Research Council of Turkey (TUBITAK - 115O381)

The effect of melatonin in rats with uterine torsion on uterus contractions, and the levels of ADMA, SDMA, arginine, Hsp90, TLR4, and NF-κB

In this study was aimed at reducing uterine damage and increasing fertility after uterine torsion in pregnant animals. With this aim, uterine torsion was experimentally formed in 35 rats that were between 18-19 days pregnant. The animals were randomly divided into five groups, and melatonin was administered prior to torsion, at the time of torsion, and detorsion (10 mg/kg/gun IP). Ovario-hysterectomy operation was performed on all animals on the first day following parturition. Subsequently, from the obtained uterus samples, determination of the levels of asymmetrical dimethyl arginin (ADMA), symmetrical dimethyl arginin (SDMA), and arginine was made using the high-performance liquid chromatography (HPLC) and levels of Heat shock protein 90 (Hsp90), TLR4 (Toll Like Receptor 4) and NF kappa B (Nuclear factor kappa-light-chain-enhancer of activated B cells) were measured using the Western blot technique. The contraction-relaxation responses of the myometrium were also determined in the organ baths. According to the results of the western blot, higher protein expressions than those of the control group were determined in the second, third, fourth, and fifth groups in Hsp90, TLR4, NF-kappa B. The lowest values of arginine and ADMA were found in Group 3, whilst the lowest SDMA value was determined in Group 1. It was determined that melatonin reduces tissue damage secondary to torsio uteri and, furthermore, that administration of this hormone at the time of torsion formation was more effective than its administration at the time of detorsion.Scientific and Technological Research Council of TurkeyTurkiye Bilimsel ve Teknolojik Arastirma Kurumu (TUBITAK) [TUBITAK - 115O381]This study was supported by the Scientific and Technological Research Council of Turkey (TUBITAK - 115O381)