GENETIC IN CANCER: RB1 MUTATIONS IN RETINOBLASTOMA CASES

Retinoblastoma (RB) is a uncommon childhood malignant ailment induced by means of the biallelic inactivation of RB1 gene. Early analysis and identification of carriers of heritable RB1 mutations can enhance sickness effect and management. In this study, mutational evaluation used to be performed on fifty-nine matched tumor and peripheral blood samples from 18 bilateral and forty one unilateral unrelated RB instances by means of a combinatorial strategy of Multiplex Ligation-dependent Probe Amplification (MLPA) assay, deletion screening, direct sequencing, replica wide variety gene dosage evaluation and methylation assays. Screening of each blood and tumor samples yielded a mutation detection charge of 94.9% (56/59) whilst solely 42.4% (25/59) of mutations had been detected if blood samples on my own had been analyzed. Biallelic mutations had been discovered in 43/59 (72.9%) of tumors screened. There have been three instances (5.1%) in which no mutations may want to be detected and germline mutations have been detected in 19.5% (8/41) of unilateral cases. A whole of sixty one factor mutations have been identified, of which 10 had been novel. There was once a excessive incidence of in the past stated recurrent mutations, taking place at 38.98% (23/59) of all cases. Of pastime had been three instances of mosaic RB1 mutations detected in the blood from sufferers with unilateral retinoblastoma. Our findings additionally underscore the significance of genetic checking out in aiding individualized disorder administration plans for sufferers and asymptomatic household individuals carrying low-penetrance, germline mosaicism or heritable unilateral mutational phenotypes

The Relationship Between Husband Support and Behavior of Pregnant Teenagers to Face Pregnancy During the Covid-19 Pandemic in Gombong, Kebumen, Indonesia

Background: Age influences pregnancy and childbirth. Pregnant women aged less than 20 years old or pregnant adolescents are not physically and mentally ready to cope with pregnancy or childbirth. The husband's support highly determines the health status of the mother. This research aimed to determine the relationship between husband support and the behavior of pregnant adolescents to face pregnancy during the COVID-19 pandemic in the Gombong District, Kebumen Regency. Methods: This study used a cross-sectional approach. The population was all pregnant women aged less than 20 years in the Gombong District, Kebumen Regency. It used a saturated sampling technique involving 256 people. Data were analyzed using univariate and bivariate analysis to answer the percentage and the Chi-Square test to answer the research hypothesis. Results: The study showed that 130 (50.8%) husbands did not support the mothers, and 126 (49.2%) husbands supported the mothers. The result also showed that 116 (45.3%) of the pregnant women showed positive behavior, while140 (54.7%) pregnant women showed negative behavior. The chi-square test found a p-value of 0.005, which proved a significant relationship between the husband's support and the behavior of pregnant adolescents to overcome pregnancy during the covid-19 pandemic. Conclusion: Pregnant teenagers with husband support will prepare for pregnancy and childbirth programs more maturely to ensure the safety of both mother and fetus

Pelatihan Terapi Endrorphine Massage Pada Ibu Hamil Tm 3 Primigravida Di Rumah Sakit Dr. Sobirin Lubuk Linggau

Universities are the pioneers in organizing the higher education process. A reliable Tri Dharma of Higher Education is the relationship between higher education and society that is established in a hamonis and mutual need. The existence of community service is actually very important for university managers and for the existence of the university itself. In order to carry out the Tri Dharma of Higher Education, the Faculty of Health Sciences, Dehesen University Bengkulu organizes an increase in health knowledge to the community, one of which is community service carried out is "Endrorphine Massage Therapy Training for Pregnant Women TM 3 Primigravida at Dr. Sobirin Lubuk Linggau Hospital". Pregnancy is a series of processes that women will experience which begins with the meeting of eggs and sperm in the ovaries followed by the formation of zygotes then growth and development until the formation of the fetus (Reeder et al, 2011). The span of pregnancy is generally about 280 days or 40 weeks. The pregnancy period is divided into three trimesters (Widatiningsih & Dewi, 2017). During pregnancy, there will be several changes, both physiological changes and psychological changes. These changes are normal for pregnant women because of the body's adjustment to the presence of the fetus in the womb. . Common physiological changes are an enlarged abdomen, active fetal movements, skin hyperpigmentation, enlarged breasts, body posture becomes lordosis (Deswani et al., 2018). Common psychological changes are anxiety, worry, fear, fantasy. anxiety will be increasingly felt especially when the mother enters the third trimester of pregnancy. This is because primigravida pregnant women will face a labor process that she has never experienced before. Protracted anxiety in pregnant women is not impossible to have a bad impact on the mother and fetus. Therefore, the need for Endrorphine Massage Therapy training is expected to increase knowledge to TM 3 Primigravida pregnant women and after this training is expected TM 3 pregnant women can practice Endrorphine Massage Therapy to prevent and control anxiety

Edukasi Pengenalan Seks Dini Pada Komunitas Anak Di Rumah Singgah Kota Bengkulu

Sexual violence is still high, which is proof of low parental knowledge to apply parenting concepts and low child knowledge in general. For decreasing sexual violence, early education about sex is a solution to improving children's knowledge and a preventive measure to protect them. Community service is proposed for the children's community under KCS Odot, Bengkulu City, Indonesia. respondents to reduce the case by proposing improved children's sex education knowledge. According to the results of community service in the KCS Odot child community in Bengkulu city, children had good knowledge among 53 respondents (60%), and low knowledge among 36 respondents (40%). According to the findings, majority  of participants had a good level of early sexual education

Social-Economic and Children’s Nutritional Status in Bengkulu Province

This study aims to determine the factors related to the nutritional status of children in Bengkulu Province due to COVID-19 that affect economic conditions and make the healthy situation worse than before, especially child nutrition. The method used is a cross-sectional study conducted through an online questionnaire. The results of this study indicate that the factors that are significantly related to the nutritional status of children are; financially inadequate (AOR = 2.32; 95% CI: 1.50 to 3.60, P<0.0001), employed (AOR = 3.03; 95% CI: 193 to 4.76, P< 0.0001), living in urban areas (AOR = 1.59; 95% CI: 1.03 – 2.43, P: 0.034), local ethnicity (AOR = 1.72; 95% CI: 1.11 to 2, 68, P: 0.016) and low-income family support (AOR = 2.10; 95% CI: 1.34 to 3.28 < P<0.0001). In conclusion, financial condition, employment status, place, ethnicity, and family are related to the nutritional status of children in Bengkulu Province. Keywords: Financial Condition, Employment, Children's Nutritional Statu

Occupational Safety Practice of Hazardous Health-care Waste Management in Bengkulu City

Background: Facing many patients while the workers were limited prompted health workers to make a quick decision regarding health-care waste. High workload pressured health workers and put them at risk of getting diseases than others, especially diseases from medical waste that they took care. Besides, as health workers, they should be clean from bacteria or viruses that can transmit  diseases to their patients. This research aimed to investigate the factors associated with occupational safety practice of hazardous health-care waste management in Bengkulu City. Method: This cross-sectional study used a stratified random sampling technique to select 230 respondents who work as health workers to respond to a questionnaire interview. The data were analyzed using multivariate logistic regression to find the association between the outcomes and the independent variables. Results: The prevalence of   poor  occupational safety practice of hazardous health-carewaste management was 35.21 % (95% CI = 29.27-41.66).  The factors significantly associated with occupational safety practice of hazardous health-care waste management were; gender (p-value < 0.001), department/unit (p-value < 0.001), knowledge (p-value < 0.001), and spirituality (p-value  < 0.001). Conclusion:  gGnder, department/unit, knowledge, and spirituality were associated with occupational safety practice of hazardous health-carewaste management in Bengkulu City

Comparison of the Concentration of Local Microorganisms (MOL) in Stale Rice During the Composting Process

Background: The symbiotic relationship between microorganisms, specifically decomposing bacteria, and organic material, such as organic waste, leads to the decomposition of the substance and its transformation into compost. Objective: The aim of this study is to assess the efficacy of local microorganisms (MOL) tape in accelerating the decomposition process of organic waste compost. Methods: This research used an experimental study strategy known as "control with posttest". This investigation was conducted utilising two distinct groups: the control group and the treatment group. Regarding the intervention group utilising indigenous microorganisms (MOL) stale rice, the research utilised two data analyses: univariate analysis, which involved presenting data in the form of frequency distribution tables, and bivariate analysis, which included conducting a one-way Anova test followed by an LSD test. Results: The findings indicated that the optimal dosage of a local microorganism (MOL) activator for compost formation was 25 ml, resulting in a duration of 11.6 days. The statistical analysis revealed a significant difference (p < 0.05) in the duration of compost formation between the dosage levels of 10 ml, 15 ml, 20 ml, and 25 ml of the local microorganism (MOL) activator. Conclusions: This research aims to explore the potential of MOL activators in accelerating the composting process of organic waste. By utilising MOL activators, it is anticipated that the problem of slow composting can be effectively addressed, offering a viable solution for the community. Moreover, the ease and affordability of producing MOL activators further enhance their practicality

Pelatihan Prosedur Keselamatan Bagi Pemandu Pariwisata Air Terjun Trisakti Desa Belitar Seberang

Indonesia adalah negara yang mempunyai ribuan pulau dengan kekayaan alam dan keaneka ragaman budaya yang menarik dan memiliki ciri khas tersendiri bagi siapa saja yang melihatnya. Indonesia memiliki obyek wisata alam yang terkenal di seluruh dunia, baik itu pantai, pegunungan, hutan wisata, air terjun, wisata goa yang potensi besar untuk menjadi salah satu negara yang menjadi tujuan wisata. Tempat wisata identik dengan bersenang-senang, namun kegiatan wisata ini juga tentunya memiliki berbagai macam resiko, seperti resiko kejadian gawat atau kecelakaan yang menimpa pelaku pariwisata baik itu pekerja maupun wisatawan. Adapun kejadian gawat darurat yang dialami di tempat wisata seperti kehilangan kesadaran karena terseret arus/tenggelam, luka, patah tulang terkilir, dislokasi lutut dan sebagainya. Dalam kejadian ini pelaku pariwisata baik itu wisatawan, penyedia jasa, pendukung wisata, pemerintah, masyarakat lokal merupakan first responder yang termasuk sebagai orang awam atau masyarakat umum. Secara spontan sebagian dari mereka melakukan pertolongan terhadap korban sesuai dengan pengetahuannya (Pro Emergency, 2011).&nbsp; Pramuwisata adalah seseorang yang dibayar untuk menemani wisatawan dalam perjalanan mengunjungi, melihat, menyaksikan objek dan atraksi wisata yang menurut wisatawan pramuwisata adalah seseorang yang bekerja pada suatu biro perjalanan atau suatu kantor pariwisata (tourism office) yang bertugas memberikan informasi, petunjuk secara langsung kepada wisatawan sebelum dan selama perjalanan

Pengaruh Roleplay Pendidikan Kesehatan Tentang Pertolongan Pertama Pada Kecelakaan (P3K) Terhadap Pengetahuan Pemandu Parawisata Di Desa Blitar Sebrang Kabupaten Rejang Lebong Tahun 2022

Background. The provision of first aid must be done quickly and accurately by using the facilities and infrastructure available at the scene. A first aid action that is done properly will reduce disability or suffering and even save the victim from death, but if the first aid action is not carried out properly it can even worsen the accident and even cause death, a tour guide must be able to provide security and safety to every tourist who visits. Health education is all activities to provide and improve the knowledge, attitudes, practices of individuals, groups or communities in maintaining and improving their own health. Purpose of this study was to determine the effect of Health Education Roleplay on First Aid in Accidents (P3K) on the Knowledge of Tourist Guides in Blitar Sebrang Village, Rejang Lebong Regency. Methods This research is analytical research with Pre Experimental Design (The One Group Pre-Test-Post Test Design). The sampling technique used stratified random sampling. The number of respondents is 35 people. Results of statistical tests before and after the intervention of health education about first aid in accidents (P3K) on the knowledge of tour guides showed a significant effect with the P-Value = 0.000 less than 0.05. Conclusion There is an Influence of Health Education Roleplay on First Aid in Accidents (P3K) on Knowledge of Tourist Guides in Blitar Sebrang Village, Rejang Lebong Regency. &nbsp