Robustesse des approches chimiométriques pour la reconstruction de profils moléculaires

Ce papier traite des approches chimiométriques pour la reconstruction de profils moléculaires. La quantification de protéines du sang est réalisée à partir du traitement par analyse factorielle de spectrogrammes issus d'une chaîne d'analyse contenant une colonne de nano-chromatographie et un spectromètre de masse. Nous nous intéressons plus particulièrement à la comparaison de la robustesse des méthodes de régression de type Unfold-PLS, N-PLS et PARAFAC vis-à-vis du problème de décalage temporel des pics contenus dans les spectrogrammes. Les méthodes multidimensionnelles type N-PLS et PARAFAC fournissent de meilleurs résultats ce qui permet d'envisager une quantification des protéines avec une plus grande tolérance sur le recalage des pics en temps de rétention

A Comparison of Radio and X-Ray Morphologies of Four Clusters of Galaxies Containing Radio Halos

Clusters of galaxies may contain cluster-wide, centrally located, diffuse radio sources, called halos. They have been found to show morphologies similar to those of the X-ray emission. To quantify this qualitative statement we performed a point-to-point comparison of the radio and the X-ray emission for four clusters of galaxies containing radio halos: Coma, Abell 2255, Abell 2319, Abell 2744. Our study leads to a linear relation between the radio and the X-ray surface brightness in two clusters, namely Abell 2255 and Abell 2744. In Coma and A2319 the radio and the X-ray brightnesses seem to be related with a sub-linear power law. Implications of these findings within simple radio halo formation models are briefly discussed.Comment: 9 pages, 13 .ps figures, accepted by A&

Exclusion and reappropriation: experiences of contemporary enclosure among children in three East Anglian schools

Transformations of the landscapes which children inhabit have significant impacts on their lives; yet, due to the limited economic visibility of children’s relationships with place, they have little stake in those transformations. Their experience, therefore, illustrates in an acute way the experience of contemporary enclosure as a mode of subordination. Following fieldwork in three primary schools in South Cambridgeshire, UK, we offer an ethnographic account of children’s experiences of socio-spatial exclusion. Yet, we suggest that such exclusion is by no means an end-point in children’s relationships with place. Challenging assumptions that children are disconnected from nature, we argue that through play and imaginative exploration of their environments, children find ways to rebuild relationships with places from which they find themselves excluded.PostprintPeer reviewe

Reconstruction bayésienne de profils moléculaires

L'étude des protéines laisse entrevoir de grands espoirs pour la médecine de demain. Cependant pour répondre à ces promesses, les méthodes actuelles doivent gagner en sensibilité, en spécificité et en robustesse. Dans cette optique, le CEA développe un laboratoire sur puce et des méthodes de traitement numérique dédiées aux analyses protéomiques par LC-MS. Nous présentons dans cet article une approche bayésienne pour la reconstruction des profils de concentrations de protéines. Dans un premier temps nous proposons un modèle pour le dispositif de mesure complet. Puis nous décrivons une méthode d'estimation des concentrations de protéines présentes, les paramètres instrumentaux étant fixés. Enfin nous estimons conjointement les concentrations et les paramètres instrumentaux

Evolution of porosity in carbide-derived carbon aerogels

Carbide-derived carbon (CDC) aerogel monoliths with very high porosity are synthesized starting from polymeric precursors. Cross-linking by platinum-catalyzed hydrosilylation of polycarbosilanes followed by supercritical drying yields preceramic aerogels. After ceramic conversion and silicon extraction in hot chlorine gas, hierarchically porous carbon materials with specific surface areas as high as 2122 m² g⁻¹ and outstanding total pore volumes close to 9 cm³ g⁻¹ are obtained. Their pore structure is controllable by the applied synthesis temperature as shown by combined nitrogen (-196 °C) and carbon dioxide (0 °C) measurements coupled with electron microscopic methods. The combination of large micropore volumes and the aerogel-type pore system leads to advanced adsorption properties due to a combination of large storage capacities and effective materials transport in comparison with purely microporous reference materials as shown by thermal response measurements

Dioxygenolytic cleavage of aryl ether bonds: 1,10-dihydro-1,10-dihydroxyfluoren-9-one, a novel arene dihydrodiol as evidence for angular dioxygenation of dibenzofuran

Two dibenzofuran degrading bacteria, Brevibacterium strain DPO 1361 and strain DPO 220, were found to utilize fluorene as sole source of carbon and energy. Cells which were grown on dibenzofuran, transformed fluorene into a number of products. For five of the seven metabolites isolated, the structure could be established unequivocally. Accumulation of one metabolite, 1,10-dihydroxy-1, 10-dihydrofluoren-9-one, indicated the presence of a novel type of dioxygenase, attacking polynuclear aromatic systems in the unusual angular position. Dibenzofuran degradation is proposed to likewise proceed via initial angular dioxygenation. Only aryl oxygen ether bond, which normally is extremely stable, is thus transformed to a hemiacetal. After spontaneous cleavage and subsequent rearomatization by dehydration, 2,2′,3-trihydroxybiphenyl [3-(2-hydroxyphenyl)-catechol] thus results as the immediate product of the first enzymatic reaction in the degradation sequence

Enrichment of dibenzofuran utilizing bacteria with high co-metabolic potential towards dibenzodioxin and other anellated aromatics

Dibenzofuran degrading bacteria were enriched from various environmental sources. A mutualistic mixed culture of strain DPO 220 and strain DPO 230 was characterized. Strain DPO 220 alone showed limited growth with dibenzofuran as sole source of carbon and energy (td ≥ 4.5 h). A labile degradation product, C12H10O5, and salicylate were isolated from the culture fluid. Salicylate was found to be a central intermediate of DBF-degradation.Strain DPO 220 co-metabolized a wide range of anellated aromatics as well as heteroaromatics. High rates of co-oxidation of dibenzodioxin demonstrate analogue-enrichment to be a powerful technique for selecting enzymatic activities for otherwise non-degradable substrates

Nanomechanics of individual aerographite tetrapods

R.A., O.L. and K.S. would like to thank the German Research Foundation (DFG) for the financial support under schemes AD 183/17-1 and SFB 986-TP-B1, respectively, and the Graphene FET Flagship. R.M. and D.E. would like to thank for financial support from Latvian Council of Science, no. 549/2012. N.M.P. is supported by the European Research Council (ERC PoC 2015 SILKENE no. 693670) and by the European Commission H2020 under the Graphene Flagship (WP14 ‘Polymer Composites’, no. 696656) and under the FET Proactive (‘Neurofibres’ no. 732344). S.S. acknowledges support from SILKENE

Degradation of haloaromatic compounds

An ever increasing number of halogenated organic compounds has been produced by industry in the last few decades. These compounds are employed as biocides, for synthetic polymers, as solvents, and as synthetic intermediates. Production figures are often incomplete, and total production has frequently to be extrapolated from estimates for individual countries. Compounds of this type as a rule are highly persistent against biodegradation and belong, as "recalcitrant" chemicals, to the class of so-called xenobiotics. This term is used to characterise chemical substances which have no or limited structural analogy to natural compounds for which degradation pathways have evolved over billions of years. Xenobiotics frequently have some common features. e.g. high octanol/water partitioning coefficients and low water solubility which makes for a high accumulation ratio in the biosphere (bioaccumulation potential). Recalcitrant compounds therefore are found accumulated in mammals, especially in fat tissue, animal milk supplies and also in human milk. Highly sophisticated analytical techniques have been developed for the detection of organochlorines at the trace and ultratrace level

Exclusion and reappropriation: experiences of contemporary enclosure among children in three East Anglian schools

Transformations of the landscapes which children inhabit have significant impacts on their lives; yet, due to the limited economic visibility of children’s relationships with place, they have little stake in those transformations. Their experience, therefore, illustrates in an acute way the experience of contemporary enclosure as a mode of subordination. Following fieldwork in three primary schools in South Cambridgeshire, UK, we offer an ethnographic account of children’s experiences of socio-spatial exclusion. Yet, we suggest that such exclusion is by no means an end-point in children’s relationships with place. Challenging assumptions that children are disconnected from nature, we argue that through play and imaginative exploration of their environments, children find ways to rebuild relationships with places from which they find themselves excluded.</p