Metalloproteinase-Mediated, Context-Dependent Function of Amphiregulin and HB-EGF in Human Keratinocytes and Skin

Human keratinocytes (KCs) express multiple EGF receptor (EGFR) ligands; however, their functions in specific cellular contexts remain largely undefined. To address this issue, first we measured mRNA and protein levels for multiple EGFR ligands in KCs and skin. Amphiregulin (AREG) was by far the most abundant EGFR ligand in cultured KCs, with >19 times more mRNA and >7.5 times more shed protein than any other family member. EGFR ligand expression in normal skin was low (<8‰ of RPLP0/36B4); however, HB-EGF and AREG mRNAs were strongly induced in human skin organ culture. KC migration in scratch wound assays was highly metalloproteinase (MP)- and EGFR dependent, and was markedly inhibited by EGFR ligand antibodies. However, lentivirus-mediated expression of soluble HB-EGF, but not soluble AREG, strongly enhanced KC migration, even in the presence of MP inhibitors. Lysophosphatidic acid (LPA)-induced ERK phosphorylation was also strongly EGFR and MP dependent and markedly inhibited by neutralization of HB-EGF. In contrast, autocrine KC proliferation and ERK phosphorylation were selectively blocked by neutralization of AREG. These data show that distinct EGFR ligands stimulate KC behavior in different cellular contexts, and in an MP-dependent fashion

Assessing Driver Acceptability of the PROSPECT Systems

Final Event of the European Projects on the Traffic Safety of Vulnerable Road Users, TARRAGONA, ESPAGNE, 12-/10/2018 - 12/10/2018Acceptance testing provides knowledge on users' perception of the systems developed within the PROSPECT project, and an indication of their likelihood to purchase such a system. Results show a high likelihood of acceptance of PROSPECT systems. Participants were most positive towards the warning function, but also indicated a high likelihood of using the braking and steering functions

Forschung über den Strafvollzug in Deutschland : die Rolle der kriminologischen Dienste (zugleich eine Replik auf Fährmann & Knop 2017)

akzeptierte ManuskriptversionManuskript weicht in der Seitenzählung vom Original ab

Deliverable 7.2. Report on methodology for balancing user acceptance, robustness and performance

The primary goal of this deliverable is to provide an overview of the methodology for acceptance testing that will be used during the tests conducted in T7.1, T7.2 and T7.3 within the PROSPECT project. The report starts with a description of the main characteristics of the most relevant accident scenarios where safety improvements are necessary. Among all use cases identified in WP3, twelve have been especially selected by the project to be implemented in the demonstrators: 9 for cyclists and 3 for pedestrians. Behaviours such as the velocity, distance and offset of the vehicle and cyclist are defined, so that the Safe Scenario, Critical Scenario and Possible Critical Scenario can be realized on the test tracks or in simulator environments. A literature review covering acceptance evaluation issues is then presented, outlining the questionnaires that are generally used to evaluate subjective measures, such as acceptance and trust. The methodology developed for Task 7.3 is then based on such questionnaires to be administered in tests and experiments that will evaluate PROSPECT systems. By using common questionnaires, this task facilitates an overall evaluation of the acceptance of all the developed functions. The methodology is presented in section 4 of this report, including a tool for data collection (LimeSurvey). This tool makes it possible for participants in evaluation studies to answer questions on various displays, to the convenience of the experimenters. In order to balance the user acceptance to the robustness and performance of the tested systems, all answers to the questionnaires will be linked to the PROSPECT functions tested and to the quality of the PROSPECT systems functioning. This methodology will be used at different times of the tests: before running a test/experiment (questionnaire 1 - participant information and questionnaire 3 - global expected acceptance of the system or a priori acceptability), during the test/experiment (questionnaire 2 - feedback on each situation) and after the test/experiment (questionnaire 3 - global acceptance of the system after having experienced it). At the end of this document, a section briefly describes all the experiments currently planned that will use the methodology within WP7. Their results will be reported in Deliverable 7.3 Report on simulator test results and driver acceptance of PROSPECT functions

D7.3 Report on simulator test results and driver acceptance of PROSPECT functions

The process of developing new automotive systems includes various testing cycles to assure a save operation in traffic. Physical system testing on test tracks is very important for this purpose, but rather expensive and might only become possible in later stages of the development process. Using a virtual simulation environment offers a safe possibility of testing new systems in early stages of development. Aditionally, driver-in-the-loop tests at test track and in a virtual simulator make it possible to evaluate driver reaction and potential acceptance by the future users of those systems. Within PROSPECT the new functions are investigated under various aspects in several simulator studies and test track studies. This deliverable D7.3 gives detailed information of conduction and results of the each study. Three studies focus exclusively on the for Vulnerable Road Users (VRUs) specifically dangerous urban intersection scenarios. The first of those studies examines the driver behaviour in a turning situation when a byciclist might be crossing. The described phenomena are looked-but-failed-to-see and failed-to-look. The second study, which provides an initial step in this line of research, analyzed the acceptance of issued forward collision warning times. The positioning of the potential accident opponent and the subjective feeling towards the criticality of the situation by the driver were key parameters taken into account. Last, but not least the acceptance of an intersection assist autonomous emergency braking systems was tested regarding the acceptance of potential buyers. The study was run for five days in a row for each participant to be able to judge the behaviour in a comuting situation. Two studies focused on longitudinal scenarios. Both studies followed the same design, but one was conducted on a test track and the other one in a simulator. The main objective was to investigate drivers reactions to FCW warnings and Active Steering interventions in critical VRU scenarios in case of a distraction of the driver. Additionally, the test track study was used to validate the results from the simulator study. The results of those studies are the basis for a wide acceptance evaluation of the systems. No system is an asset in increasing road safety if it is not accepted by the user and therefore turned off, if it is not required the system to be default on in consumer tests. Complemented by an additional acceptance study where the participants had to give their opinion of those systems after they watched videos of dangerous situations, the acceptance was analyzed based on questionnaires developed in PROSPECT and reported in Deliverable 7.2. This wholistic approach allows an expert discussion on the potentials of the PROSPECT functions in the future

Assessment of the PROSPECT safety systems including socio-economic evaluation

This report provides a new methodology for safety benefit assessment of real-world benefit of the Advanced Driver Assistance Systems (ADAS) in terms of saved lives and prevented injuries as well as the resulting monetary benefit for society. This methodology is demonstrated and applied to PROSPECT systems that address potential crashes of passenger cars with vulnerable road users (VRUs) such as pedestrians and cyclists

Exorcising Grice’s ghost : an empirical approach to studying intentional communication in animals

Language’s intentional nature has been highlighted as a crucial feature distinguishing it from other communication systems. Specifically, language is often thought to depend on highly structured intentional action and mutual mindreading by a communicator and recipient. Whilst similar abilities in animals can shed light on the evolution of intentionality, they remain challenging to detect unambiguously. We revisit animal intentional communication and suggest that progress in identifying analogous capacities has been complicated by (i) the assumption that intentional (that is, voluntary) production of communicative acts requires mental-state attribution, and (ii) variation in approaches investigating communication across sensory modalities. To move forward, we argue that a framework fusing research across modalities and species is required. We structure intentional communication into a series of requirements, each of which can be operationalised, investigated empirically, and must be met for purposive, intentionally communicative acts to be demonstrated. Our unified approach helps elucidate the distribution of animal intentional communication and subsequently serves to clarify what is meant by attributions of intentional communication in animals and humans

Global maps of soil temperature

Research in global change ecology relies heavily on global climatic grids derived from estimates of air temperature in open areas at around 2 m above the ground. These climatic grids do not reflect conditions below vegetation canopies and near the ground surface, where critical ecosystem functions occur and most terrestrial species reside. Here, we provide global maps of soil temperature and bioclimatic variables at a 1-km² resolution for 0–5 and 5–15 cm soil depth. These maps were created by calculating the difference (i.e., offset) between in-situ soil temperature measurements, based on time series from over 1200 1-km² pixels (summarized from 8500 unique temperature sensors) across all the world’s major terrestrial biomes, and coarse-grained air temperature estimates from ERA5-Land (an atmospheric reanalysis by the European Centre for Medium-Range Weather Forecasts). We show that mean annual soil temperature differs markedly from the corresponding gridded air temperature, by up to 10°C (mean = 3.0 ± 2.1°C), with substantial variation across biomes and seasons. Over the year, soils in cold and/or dry biomes are substantially warmer (+3.6 ± 2.3°C) than gridded air temperature, whereas soils in warm and humid environments are on average slightly cooler (-0.7 ± 2.3°C). The observed substantial and biome-specific offsets emphasize that the projected impacts of climate and climate change on near-surface biodiversity and ecosystem functioning are inaccurately assessed when air rather than soil temperature is used, especially in cold environments. The global soil-related bioclimatic variables provided here are an important step forward for any application in ecology and related disciplines. Nevertheless, we highlight the need to fill remaining geographic gaps by collecting more in-situ measurements of microclimate conditions to further enhance the spatiotemporal resolution of global soil temperature products for ecological applications

Global maps of soil temperature

Research in global change ecology relies heavily on global climatic grids derived from estimates of air temperature in open areas at around 2 m above the ground. These climatic grids do not reflect conditions below vegetation canopies and near the ground surface, where critical ecosystem functions occur and most terrestrial species reside. Here, we provide global maps of soil temperature and bioclimatic variables at a 1-km2 resolution for 0–5 and 5–15 cm soil depth. These maps were created by calculating the difference (i.e. offset) between in situ soil temperature measurements, based on time series from over 1200 1-km2 pixels (summarized from 8519 unique temperature sensors) across all the world\u27s major terrestrial biomes, and coarse-grained air temperature estimates from ERA5-Land (an atmospheric reanalysis by the European Centre for Medium-Range Weather Forecasts). We show that mean annual soil temperature differs markedly from the corresponding gridded air temperature, by up to 10°C (mean = 3.0 ± 2.1°C), with substantial variation across biomes and seasons. Over the year, soils in cold and/or dry biomes are substantially warmer (+3.6 ± 2.3°C) than gridded air temperature, whereas soils in warm and humid environments are on average slightly cooler (−0.7 ± 2.3°C). The observed substantial and biome-specific offsets emphasize that the projected impacts of climate and climate change on near-surface biodiversity and ecosystem functioning are inaccurately assessed when air rather than soil temperature is used, especially in cold environments. The global soil-related bioclimatic variables provided here are an important step forward for any application in ecology and related disciplines. Nevertheless, we highlight the need to fill remaining geographic gaps by collecting more in situ measurements of microclimate conditions to further enhance the spatiotemporal resolution of global soil temperature products for ecological applications