3 research outputs found
Delayed internalization and lack of recycling in a beta<sub>2</sub>-adrenergic receptor fused to the G protein alpha-subunit
<p>Abstract</p> <p>Background</p> <p>Chimeric proteins obtained by the fusion of a G protein-coupled receptor (GPCR) sequence to the N-terminus of the G protein α-subunit have been extensively used to investigate several aspects of GPCR signalling. Although both the receptor and the G protein generally maintain a fully functional state in such polypeptides, original observations made using a chimera between the β<sub>2</sub>-adrenergic receptor (β<sub>2</sub>AR) and Gα<sub>s </sub>indicated that the fusion to the α-subunit resulted in a marked reduction of receptor desensitization and down-regulation. To further investigate this phenomenon, we have compared the rates of internalization and recycling between wild-type and Gα<sub>s</sub>-fused β<sub>2</sub>AR.</p> <p>Results</p> <p>The rate of agonist-induced internalization, measured as the disappearance of cell surface immunofluorescence in HEK293 cells permanently expressing N-terminus tagged receptors, was reduced three-fold by receptor-G protein fusion. However, both fused and non-fused receptors translocated to the same endocytic compartment, as determined by dual-label confocal analysis of cells co-expressing both proteins and transferrin co-localization.</p> <p>Receptor recycling, determined as the reversion of surface immunofluorescence following the addition of antagonist to cells that were previously exposed to agonist, markedly differed between wild-type and fused receptors. While most of the internalized β<sub>2</sub>AR returned rapidly to the plasma membrane, β<sub>2</sub>AR-Gα<sub>s </sub>did not recycle, and the observed slow recovery for the fusion protein immunofluorescence was entirely accounted for by protein synthesis.</p> <p>Conclusion</p> <p>The covalent linkage between β<sub>2</sub>AR and Gα<sub>s </sub>does not appear to alter the initial endocytic translocation of the two proteins, although there is reduced efficiency. It does, however, completely disrupt the process of receptor and G protein recycling. We conclude that the physical separation between receptor and Gα is not necessary for the transit to early endosomes, but is an essential requirement for the correct post-endocytic sorting and recycling of the two proteins.</p