View and Illumination Invariant Object Classification Based on 3D Color Histogram Using Convolutional Neural Networks

Object classification is an important step in visual recognition and semantic analysis of visual content. In this paper, we propose a method for classification of objects that is invariant to illumination color, illumination direction and viewpoint based on 3D color histogram. A 3D color histogram of an image is represented as a 2D image, to capture the color composition while preserving the neighborhood information of color bins, to realize the necessary visual cues for classification of objects. Also, the ability of convolutional neural network (CNN) to learn invariant visual patterns is exploited for object classification. The efficacy of the proposed method is demonstrated on Amsterdam Library of Object Images (ALOI) dataset captured under various illumination conditions and angles-of-view

Effects of the Insemination of Hydrogen Peroxide-Treated Epididymal Mouse Spermatozoa on γH2AX Repair and Embryo Development

BACKGROUND: Cryopreservation of human semen for assisted reproduction is complicated by cryodamage to spermatozoa caused by excessive reactive oxygen species (ROS) generation. METHODS AND FINDINGS: We used exogenous ROS (H(2)O(2)) to simulate cryopreservation and examined DNA damage repair in embryos fertilized with sperm with H(2)O(2)-induced DNA damage. Sperm samples were collected from epididymis of adult male KM mice and treated with capacitation medium (containing 0, 0.1, 0.5 and 1 mM H(2)O(2)) or cryopreservation. The model of DNA-damaged sperm was based on sperm motility, viability and the expression of γH2AX, the DNA damage-repair marker. We examined fertility rate, development, cell cleavage, and γH2AX level in embryos fertilized with DNA-damaged sperm. Cryopreservation and 1-mM H(2)O(2) treatment produced similar DNA damage. Most of the one- and two-cell embryos fertilized with DNA-damaged sperm showed a delay in cleavage before the blastocyst stage. Immunocytochemistry revealed γH2AX in the one- and four-cell embryos. CONCLUSIONS: γH2AX may be involved in repair of preimplantation embryos fertilized with oxygen-stressed spermatozoa