"Sleep disparity" in the population: poor sleep quality is strongly associated with poverty and ethnicity

<p>Abstract</p> <p>Background</p> <p>Little is known about the social determinants of sleep attainment. This study examines the relationship of race/ethnicity, socio-economic status (SES) and other factors upon sleep quality.</p> <p>Methods</p> <p>A cross-sectional survey of 9,714 randomly selected subjects was used to explore sleep quality obtained by self-report, in relation to socioeconomic factors including poverty, employment status, and education level. The primary outcome was poor sleep quality. Data were collected by the Philadelphia Health Management Corporation.</p> <p>Results</p> <p>Significant differences were observed in the outcome for race/ethnicity (African-American and Latino versus White: unadjusted OR = 1.59, 95% CI 1.24-2.05 and OR = 1.65, 95% CI 1.37-1.98, respectively) and income (below poverty threshold, unadjusted OR = 2.84, 95%CI 2.41-3.35). In multivariable modeling, health indicators significantly influenced sleep quality most prominently in poor individuals. After adjusting for socioeconomic factors (education, employment) and health indicators, the association of income and poor sleep quality diminished, but still persisted in poor Whites while it was no longer significant in poor African-Americans (adjusted OR = 1.95, 95% CI 1.47-2.58 versus OR = 1.16, 95% CI 0.87-1.54, respectively). Post-college education (adjusted OR = 0.47, 95% CI 0.31-0.71) protected against poor sleep.</p> <p>Conclusions</p> <p>A "sleep disparity" exists in the study population: poor sleep quality is strongly associated with poverty and race. Factors such as employment, education and health status, amongst others, significantly mediated this effect only in poor subjects, suggesting a differential vulnerability to these factors in poor relative to non-poor individuals in the context of sleep quality. Consideration of this could help optimize targeted interventions in certain groups and subsequently reduce the adverse societal effects of poor sleep.</p

Earthworm biomarker responses on exposure to commercial cypermethrin

© 2013 Wiley Periodicals, Inc. Cypermethrin is a synthetic pyrethroid insecticide used worldwide in agriculture, home pest control, disease vector control, and food safety. It accumulates in soil. Therefore, traces of cypermethrin may frequently appear in vegetables grown in contaminated soil. There is a push now to develop biomarkers as early warning indicators of environmental pollution. In this study, DNA damage (tail DNA%, tail length, and olive tail moment), the micronucleus, neutral red retention (NRR) time, and pinocytic adherence ability of coelomocytes were investigated in Pheretima peguana earthworms exposed to cypermethrin in filter paper tests. The NRR time of earthworm coelomocytes decreased significantly at a concentration of 3.5 × 10⁻³ μg · cm⁻² (1/100 LC₅₀) after 48 h exposure, with a highly negative correlation with cypermethrin concentration. Pinocytic adherence ability of coelomocytes also declined significantly at a cypermethrin concentration of 3.5 × 10⁻² μg · cm⁻² (1/10 LC₅₀). The DNA damage to earthworm coelomocytes (tail DNA%, tail length, and olive tail moment) increased considerably at the highest concentration (3.5 × 10⁻¹ μg · cm⁻²) although the correlation between tail DNA% and cypermethrin concentration was low. Thus, physiological biomarkers were more sensitive than the genotoxic effects in earthworms exposed to commercial cypermethrin. Although a suite of earthworm biomarkers could be used to evaluate cypermethrin terrestrial pollution, the NRR test is easier to conduct and a more sensitive indicator

Genotoxic effects of glyphosate or paraquat on earthworm coelomocytes

The potential genotoxicity (nuclear anomalies, damage to single-strand DNA) and pinocytic adherence activity of two (glyphosate-based and paraquat-based) commercial herbicides to earthworm coelomocytes (immune cells in the coelomic cavity) were assessed. Coelomocytes were extracted from earthworms (Pheretima peguana) exposed to concentrations <LC50 of glyphosate-based or paraquat-based herbicides on filter paper for 48 h. Three assays were performed: Micronucleus (light microscopy count of micronuclei, binuclei, and trinuclei), Comet (epifluorescent microscope and LUCIA image analyzer measure of tail DNA %, tail length, and tail moment), and Neutral Red (to detect phagocytic or pinocytic activity). The LC50 value for paraquat was 65-fold lower than for glyphosate indicating that paraquat was far more acutely toxic to P. peguana. There were significant (P < 0.05) differences from the control group in total coelomocyte micronuclei, binuclei, and trinuclei frequencies of earthworms exposed to glyphosate at 25 × 10⁻¹ (10⁻³ LC50) and paraquat at 39 × 10⁻⁵ (10⁻⁴ LC50) μg cm⁻² filter paper. In earthworms exposed to glyphosate, no differences in tail DNA%, tail length, and tail moment of coelomocytes were detected. In contrast, for paraquat at 10⁻¹ LC50 concentration, there were significant (P < 0.05) differences between tail DNA % and tail length, and at LC50 concentration, tail moment was also significantly different when compared with controls. A decline in pinocytic adherence activity in coelomocytes occurred on exposure to glyphosate or paraquat at 10⁻³ LC50 concentration. This study showed that, at concentrations well below field application rates, paraquat induces both clastogenic and aneugenic effects on earthworm coelomocytes whereas glyphosate causes only aneugenic effects and therefore does not pose a risk of gene mutation in this earthworm

Effect of fucoidan from Turbinaria conoides on human lung adenocarcinoma epithelial (A549) cells

Fucoidan was purified from seaweed, Turbinaria conoides. Isolated fragments were characterized with NMR (13C, 1H), Gas Chromatography–Mass Spectronomy (GC–MS) and HPLC analysis. The autohydrolysate of fucoidans consisted of sulfated fuco-oligosaccharides having the backbone of α-(1, 3)-linked fuco-pyranose derivatives and minor components of galactose, glucose, mannose and xylose sugars. Fucoidan induced a dose-dependent reduction in cell survival of lung cancer A549 cells by MTT assay (GI50, 75 μg/mL). However, it was not cytotoxic to a non-tumorigenic human keratinocyte cell line of skin tissue (HaCaT) (GI50 > 1.0 mg/mL). The apoptotic cells in fucoidan-treated A549 cells were visualized by laser confocal microscopy and cell cycle analysis showed induction of G0/G1 phase arrest of the cell progression cycle. Further, CFSE labeling and flow cytometry highlighted that fucoidan significantly (P < 0.05) inhibited the proliferation rate of A549 cells by up to 2-fold compared with the control cells. It is concluded that fucoidan has the potential to act as an anti-proliferative agent on lung carcinoma (A549) cells

Nutritional and toxicological studies of New Zealand Cookia sulcata

Interest in snails as a source of protein and as a delicacy is increasing in many countries. The present study investigated selected nutritional (proximate, amino acid, fatty acid, vitamin E, cholesterol and macro- and trace minerals) and toxic (toxic elements and organochlorine) concentrations of small and large (≤60 and > 60. g whole animal weight, respectively) Captain Cook snails (Cookia sulcata). The major amino acids in C. sulcata muscle were glutamic (13.9. g/100. g protein), arginine (10.2. g/100. g protein), glycine (9.5. g/100. g protein) and taurine (9.5. g/100. g protein). There was no difference in the amino acid profiles related to the snail size. C. sulcata had relatively high amounts of saturated fatty acids (44.4%) and polyunsaturated fatty acids (34.3%), and lesser amounts of mono-unsaturated fatty acids. The major fatty acids detected in C. sulcata were C16:0, C18:0, C20:4 and C22:5, which accounted for more than 60% of the total fatty acids. Snail size had a significant (P < . 0.05) effect on the C16:0 and C18:3 concentrations. The only isoform of vitamin E present in C. sulcata was identified as α-tocopherol at 2.16 and 3.71. mg/100. g fresh weight for the small and large snails, respectively. The average cholesterol concentration in C. sulcata was 1.33. mg/100. g fresh weight. The results indicated that none of the toxic elements, including Al, Ni, As and Pb of C. sulcata, were over the maximum concentration allowed in the Australia New Zealand Food Standard; and the organochlorine pesticides concentrations in C. sulcata were below the detection limit ( < 0.0005. mg/kg). C. sulcata could, therefore, be utilized for special dietary applications requiring higher amounts of Fe, Zn, taurine and tryptophan

Seasonal changes in leaf and stem loline alkaloids in meadow fescue

Leaf and stem loline alkaloid concentration in 10 European meadow fescue (Festuca pratensis Huds.) lines grown in a field in Canterbury, New Zealand, were determined in samples collected six times between early spring 2004 and late autumn 2005. Significant differences in loline alkaloid concentrations were noted between lines and between harvest times. Higher total loline alkaloid concentrations (up to 4990 µg g⁻¹) were found in stems compared to leaf (up to 1770 µg g⁻¹). However, the seasonal accumulation pattern of different loline alkaloid concentrations in leaf and stem varied. In most lines, stem loline concentration peaked sharply in late spring and declined during early summer and autumn. The seasonal pattern of leaf loline alkaloid concentration followed the stem concentration except for a sharp decline in early summer followed by an increase in late summer. In most instances, the concentration of N-formyl loline was the highest > N-acetyl loline > N-acetyl norloline > N-methyl loline. The possible role of stem and leaf loline alkaloids to deter pasture-feeding insects is briefly discussed

Nutritional composition of Mutton bird (Puffinus griseus) meat

Mutton birds (Puffinus griseus) are wild seabird chicks traditionally harvested by Maori but available commercially for seasonal consumption in New Zealand. Little information is available on the nutritional content of the meat from these birds. Proximate analysis and amino and fatty acid composition of Mutton bird breast meat (MBBM) were measured over two harvesting seasons, 2007 and 2008. Protein content was lower, and fat and ash contents were higher (P < 0.05) in meat from birds harvested in 2008 (18.5, 13.0 and 11.7%, respectively) compared with that from 2007 (20.3, 11.8 and 10.3%, respectively). Higher lysine concentrations and lower proline, cysteine and methionine were found in MBBM compared with literature values for beef, lamb and pork. The essential amino acid content in Mutton bird (41.7 and 38.4% for 2008 and 2007, respectively) was slightly lower than those reported for common meats (42–43%). Palmitic, arachidonic, DHA, stearic, EPA, and oleic were the major fatty acids (FA) detected in MBBM and accounted for approximately 60% of the FA. The cholesterol concentration was not affected by season. Seasonal variations MBBM existed which may be of little nutritional consequence but might be a useful indicator for ecological events including changing feed availability

Preparation of T-2-glucoronide with rat hepatic microsomes and its use along with T-2 for activation of the JAK/STAT signaling pathway in RAW264.7 cells

T-2 toxin (T-2), one of the most toxic trichothecene A-type mycotoxins, is biotransformed in animal tissues to modified T-2s (mT-2s) including T-2 glucuronide (T-2-GlcA). In this study, the optimal conditions for T-2-GlcA synthesis were established, and the JAK/STAT pathway in RAW264.7 cells was used to study the toxicity of T-2-GlcA. Because many mT-2 standards are not readily available, optimal conditions for T-2-GlcA synthesis in vitro were established by incubating T-2 with rat liver microsomes, UDPGA, and 0.2% Triton X-100 for 90 min. qRT-PCR and Western blot results showed 21- and 760-fold increases in IL-6 mRNA expression induced by T-2-GlcA and T-2, respectively. Similar differences were observed in JAK3, SOCS2/3, and CIS mRNA expression. T-2-GlcA induced a dose-responsive decrease in STAT1 mRNA expression, whereas the result with T-2 was the opposite. Moreover, the phosphorylation of STAT3 induced by T-2-GlcA was higher than that by T- 2, whereas the phosphorylation of STAT1 was to the contrary. Overall, the results show that T-2-GlcA was somewhat toxic, but activation of the JAK/STAT pathway in RAW264.7 was higher by T-2