Microstructural and geochemical constraints on the evolution of deep arc lithosphere

Mantle xenoliths from the Sierra Nevada, California, USA, sampled a vertical column (60–120 km) of lithosphere that formed during Mesozoic continental arc magmatism. This lithosphere experienced an anticlockwise P-T-t path resulting in rapid cooling that effectively “quenched in” features inherited from earlier high-temperature conditions. Here we combine new mineral chemistry data (water, trace element, and major element concentrations) with mineral crystallographic preferred orientations (CPOs) to investigate the relationship between melt infiltration and deformation. The peridotites record a refertilization trend with increasing depth, starting from shallow, coarse-protogranular, less-melt-infiltrated spinel peridotite with strong, orthorhombic olivine CPO to deep, fine-porphyroclastic, highly melt-infiltrated garnet peridotite with weak, axial-[010] olivine CPO. In contrast to the observed axial-[010] CPOs, subgrain boundary orientations and misorientation axes suggest the dominant activation of the (001)[100] slip system, suggesting deformation under moderately hydrous conditions. After accounting for effects of subsolidus cooling, we see coherent trends between mineral trace element abundance and water content, indicating that melt infiltration led to an increase in water content of the peridotites. However, measured olivine and pyroxene water contents in all peridotites (5–10 and 30–500 wt ppm, respectively) are lower than that required to promote dominant (001)[100] slip system observed in both natural and experimental samples. These results suggest that deformation occurred earlier along the P-T path, probably during or shortly after hydrous melt infiltration. Subsequent rapid cooling at 90 Ma led to water loss from olivine (owing to decreased solubility at low temperature), leaving behind a deep arc lithosphere that remained viscously coupled to the Farallon slab until the opening of the slab window in the late Cenozoic

Microstructural and geochemical constraints on the evolution of deep arc lithosphere

Mantle xenoliths from the Sierra Nevada, California, USA, sampled a vertical column (60–120 km) of lithosphere that formed during Mesozoic continental arc magmatism. This lithosphere experienced an anticlockwise P-T-t path resulting in rapid cooling that effectively “quenched in” features inherited from earlier high-temperature conditions. Here we combine new mineral chemistry data (water, trace element, and major element concentrations) with mineral crystallographic preferred orientations (CPOs) to investigate the relationship between melt infiltration and deformation. The peridotites record a refertilization trend with increasing depth, starting from shallow, coarse-protogranular, less-melt-infiltrated spinel peridotite with strong, orthorhombic olivine CPO to deep, fine-porphyroclastic, highly melt-infiltrated garnet peridotite with weak, axial-[010] olivine CPO. In contrast to the observed axial-[010] CPOs, subgrain boundary orientations and misorientation axes suggest the dominant activation of the (001)[100] slip system, suggesting deformation under moderately hydrous conditions. After accounting for effects of subsolidus cooling, we see coherent trends between mineral trace element abundance and water content, indicating that melt infiltration led to an increase in water content of the peridotites. However, measured olivine and pyroxene water contents in all peridotites (5–10 and 30–500 wt ppm, respectively) are lower than that required to promote dominant (001)[100] slip system observed in both natural and experimental samples. These results suggest that deformation occurred earlier along the P-T path, probably during or shortly after hydrous melt infiltration. Subsequent rapid cooling at 90 Ma led to water loss from olivine (owing to decreased solubility at low temperature), leaving behind a deep arc lithosphere that remained viscously coupled to the Farallon slab until the opening of the slab window in the late Cenozoic

Earth's oldest mantle fabrics indicate Eoarchaean subduction

The extension of subduction processes into the Eoarchaean era (4.0-3.6 Ga) is controversial. The oldest reported terrestrial olivine, from two dunite lenses within the ~3,720 Ma Isua supracrustal belt in Greenland, record a shape-preferred orientation of olivine crystals defining a weak foliation and a well-defined lattice-preferred orientation (LPO). [001] parallel to the maximum finite elongation direction and (010) perpendicular to the foliation plane define a B-type LPO. In the modern Earth such fabrics are associated with deformation of mantle rocks in the hanging wall of subduction systems; an interpretation supported by experiments. Here we show that the presence of B-type fabrics in the studied Isua dunites is consistent with a mantle origin and a supra-subduction mantle wedge setting, the latter supported by compositional data from nearby mafic rocks. Our results provide independent microstructural data consistent with the operation of Eoarchaean subduction and indicate that microstructural analyses of ancient ultramafic rocks provide a valuable record of Archaean geodynamics

bantam Is Required for Optic Lobe Development and Glial Cell Proliferation

microRNAs (miRNAs) are small, conserved, non-coding RNAs that contribute to the control of many different cellular processes, including cell fate specification and growth control. Drosophila bantam, a conserved miRNA, is involved in several functions, such as stimulating proliferation and inhibiting apoptosis in the wing disc. Here, we reported the detailed expression pattern of bantam in the developing optic lobe, and demonstrated a new, essential role in promoting proliferation of mitotic cells in the optic lobe, including stem cells and differentiated glial cells. Changes in bantam levels autonomously affected glial cell number and distribution, and non-autonomously affected photoreceptor neuron axon projection patterns. Furthermore, we showed that bantam promotes the proliferation of mitotically active glial cells and affects their distribution, largely through down regulation of the T-box transcription factor, optomotor-blind (omb, Flybase, bifid). Expression of omb can rescue the bantam phenotype, and restore the normal glial cell number and proper glial cell positioning in most Drosophila brains. These results suggest that bantam is critical for maintaining the stem cell pools in the outer proliferation center and glial precursor cell regions of the optic lobe, and that its expression in glial cells is crucial for their proliferation and distribution

The SUMO Isopeptidase Ulp2p Is Required to Prevent Recombination-Induced Chromosome Segregation Lethality following DNA Replication Stress

SUMO conjugation is a key regulator of the cellular response to DNA replication stress, acting in part to control recombination at stalled DNA replication forks. Here we examine recombination-related phenotypes in yeast mutants defective for the SUMO de-conjugating/chain-editing enzyme Ulp2p. We find that spontaneous recombination is elevated in ulp2 strains and that recombination DNA repair is essential for ulp2 survival. In contrast to other SUMO pathway mutants, however, the frequency of spontaneous chromosome rearrangements is markedly reduced in ulp2 strains, and some types of rearrangements arising through recombination can apparently not be tolerated. In investigating the basis for this, we find DNA repair foci do not disassemble in ulp2 cells during recovery from the replication fork-blocking drug methyl methanesulfonate (MMS), corresponding with an accumulation of X-shaped recombination intermediates. ulp2 cells satisfy the DNA damage checkpoint during MMS recovery and commit to chromosome segregation with similar kinetics to wild-type cells. However, sister chromatids fail to disjoin, resulting in abortive chromosome segregation and cell lethality. This chromosome segregation defect can be rescued by overproducing the anti-recombinase Srs2p, indicating that recombination plays an underlying causal role in blocking chromatid separation. Overall, our results are consistent with a role for Ulp2p in preventing the formation of DNA lesions that must be repaired through recombination. At the same time, Ulp2p is also required to either suppress or resolve recombination-induced attachments between sister chromatids. These opposing defects may synergize to greatly increase the toxicity of DNA replication stress

Recommendations and guidelines from the ISMRM Diffusion Study Group for preclinical diffusion MRI: Part 1 -- In vivo small-animal imaging

The value of in vivo preclinical diffusion MRI (dMRI) is substantial. Small-animal dMRI has been used for methodological development and validation, characterizing the biological basis of diffusion phenomena, and comparative anatomy. Many of the influential works in this field were first performed in small animals or ex vivo samples. The steps from animal setup and monitoring, to acquisition, analysis, and interpretation are complex, with many decisions that may ultimately affect what questions can be answered using the data. This work aims to serve as a reference, presenting selected recommendations and guidelines from the diffusion community, on best practices for preclinical dMRI of in vivo animals. In each section, we also highlight areas for which no guidelines exist (and why), and where future work should focus. We first describe the value that small animal imaging adds to the field of dMRI, followed by general considerations and foundational knowledge that must be considered when designing experiments. We briefly describe differences in animal species and disease models and discuss how they are appropriate for different studies. We then give guidelines for in vivo acquisition protocols, including decisions on hardware, animal preparation, imaging sequences and data processing, including pre-processing, model-fitting, and tractography. Finally, we provide an online resource which lists publicly available preclinical dMRI datasets and software packages, to promote responsible and reproducible research. An overarching goal herein is to enhance the rigor and reproducibility of small animal dMRI acquisitions and analyses, and thereby advance biomedical knowledge.Comment: 69 pages, 6 figures, 1 tabl

Multifactorial Regulation of a Hox Target Gene

Hox proteins play fundamental roles in controlling morphogenetic diversity along the anterior–posterior body axis of animals by regulating distinct sets of target genes. Within their rather broad expression domains, individual Hox proteins control cell diversification and pattern formation and consequently target gene expression in a highly localized manner, sometimes even only in a single cell. To achieve this high-regulatory specificity, it has been postulated that Hox proteins co-operate with other transcription factors to activate or repress their target genes in a highly context-specific manner in vivo. However, only a few of these factors have been identified. Here, we analyze the regulation of the cell death gene reaper (rpr) by the Hox protein Deformed (Dfd) and suggest that local activation of rpr expression in the anterior part of the maxillary segment is achieved through a combinatorial interaction of Dfd with at least eight functionally diverse transcriptional regulators on a minimal enhancer. It follows that context-dependent combinations of Hox proteins and other transcription factors on small, modular Hox response elements (HREs) could be responsible for the proper spatio-temporal expression of Hox targets. Thus, a large number of transcription factors are likely to be directly involved in Hox target gene regulation in vivo