"Conselhos ao povo": educação contra a influenza de 1918

Quando os primeiros casos de gripe espanhola começaram a vitimar os paulistanos em outubro de 1918, o Serviço Sanitário do Estado de São Paulo publicou uma série de prescrições com a intenção de esclarecer e instruir os moradores da capital do estado sobre a doença, suas características e algumas formas de combate de uma moléstia também chamada de influenza ou influenza espanhola. Resumidos e reeditados na imprensa, muitas vezes sob o título de "Conselhos ao Povo", os pareceres elaborados pela diretoria de saúde ganharam diferentes nuanças, mas algo permaneceu constante: o apelo à higiene pessoal e ao cuidado com os contatos sociais, como maneiras de se evitar a enfermidade e sua propagação. O objetivo deste texto é recuperar um pouco dessa história, em que as questões de higiene, educação e saúde se mesclavam

Purification and Characterization of a Population of EGFP-Expressing Cells from the Developing Pancreas of a Neurogenin3/EGFP Transgenic Mouse

Neurogenin 3 (ngn3) is a basic helix loop helix transcription factor that is transiently expressed in the developing mouse pancreas with peak expression around E15. In mice lacking the ngn3 gene the endocrine cells of the pancreas fail to develop suggesting that the ngn3-positive cell may represent a progenitor cell for the endocrine pancreas. In order to purify and characterize this cell in detail we have generated a transgenic mouse, in which the ngn3 promoter drives expression of enhanced green fluorescent protein (EGFP). In the E15.5 embryo EGFP was expressed in the dorsal and ventral pancreas, the duodenum, and lower intestine as well as in the brain. This pattern of expression was in keeping with the known expression profile of the endogenous ngn3 gene. Within the pancreas EGFP was localized in close proximity to cells that stained positive for ngn3, insulin, and glucagon, but was absent from regions of the pancreas that stained positive for amylase. EGFP was also present in the pancreas at E18.5, although there was no detectable expression of ngn3. At this stage EGFP did not colocalize with any of the hormones or exocrine markers. EGFP+ cells were FACS purified (96%) from the E15 pancreas yielding ∼ 10,000 cells or 1.6% of the total pancreatic cells from one litter. RT/PCR analysis confirmed that the purified cells expressed EGFP, ngn3, insulin, glucagon, somatostatin and pancreatic polypeptide. The ability to purify ngn3+ cells provides an invaluable source of material for charactering in detail their properties