HPLC ANALYSIS OF AMINO ACIDS CONTENT IN CRAMBE CORDIFOLIA AND CRAMBE KOKTEBELICA LEAVES

Objective: The aim of our study was to establish the content of some primary metabolites, such as amino acids in Crambe cordifolia and Crambe koktebelica. The lack of experimental data induced us to determine these compounds. Methods: Crambe cordifolia and Crambe koktebelica leaves were selected as the objects of the study. The amino acids in the raw materials were determined by the HPLC method. Results: The results of the research revealed that the leaves of Crambe cordifolia and Crambe koktebelica contain fifteen and sixteen free amino acids respectively. Among the free amino acids L-histidine was presented in Crambe cordifolia leaves in the greatest amount, its content was 12.19 µg/mg. The content of free L-arginine, L-valine, L-phenylalanine, L-isoleucine was the greatest in Crambe koktebelica leaves, it was 2.23 µg/mg, 2.04 µg/mg, 1.74 µg/mg, 1.50 µg/mg respectively. The content of bound L-glutamic acid, Glycine, L-arginine, L-leucine was the highest in Crambe cordifolia and Crambe koktebelica leaves. Conclusion: The results of the study showed that Crambe cordifolia and Crambe koktebelica can be considered as a source of highly digestible amino acids that can be used to treat some diseases

Clinical markers of immune disorders in the pathogenesis of Escherichia coli enteritis

Background. Escherichia coli enteritis is one of the most common causes of diarrhea in developed countries and is caused usually by pathogenic strains of Escherichia coli. Objectives. To investigate the role of reactive response of polymorphonuclear neutrophilic granulocytes (NG) of peripheral blood in the systemic inflammatory response mechanisms of acute Escherichia enterocolitis (AEC), depending on genes polymorphism of heat shock proteins (HSP) family 70-2 (HSP70-2, 1267A®G) and interleukin 10 (IL-10, C-592A). Material and methods. The genes polymorphism was analysed by PCR based method in 95 patients with AEC and 30 healthy individuals. Clinical markers of immune disorders were evaluated after hematological indices, based upon an extended general clinical blood analysis, using verified formulas. Results. The endogenous intoxication severity did not depend reliably on genotypes of IL-10 gene (rs1800872), however it was significantly 23.68% (р=0.043) higher in GG-genotype carriers of HSP70-2 gene (rs1061581). The reduction of cellular reactivity by 14.71-19.08% (р<0.01) did not depend on the analyzed genes genotypes. But general non-specific immune reactivity decreases 3.49-4.24 times (р<0.001) was deeper in GG-genotype carriers of HSP70-2 gene and AA-genotype carriers of IL-10 gene by 17.78% (р=0.009) and 12.37% (р=0.023) respectively. The immunologic resistance index was lower by 18.75% (р=0.024) in GG-genotype carriers than in patients with А allele. Conclusions. Hematological indices, based upon an extended general clinical blood analysis, are indicative and reliable non-specific clinical markers of immune disorders in case of AEC

Analysis of carboxylic acids of Crambe cordifolia Steven

Crambe cordifolia Steven is a perennial herb and contains many biologically active substances, including amino acids, quercetin and glycosides of kaempferol. In continuation of the investigation of these plant compounds, it is advisable to study the qualitative composition and quantitative contents of carboxylic acids. Using a HPLC method the quantitative content of the following organic acids was identified and determined: pyruvic (40.66 mg/g), isocitric (12.88 mg/g), citric (8.71 mg/g), succinic (38.03 mg/g) and malic (0.75 mg/g). Among fatty acids the saturated and unsaturated acids were determined by the GC/MS method. The content of polyunsaturated fatty acids of the total fatty acids was 56.97%, saturated – 38.53% and monounsaturated – 4.50%. Linolenic and palmitic acids dominated among the determined 7 fatty acids, their content was 9.68 mg/g (47.87%) and 4.88 mg/g (24.14%). The results of the study show that Crambe cordifolia Steven leaves is a source of carboxylic acids

HPLC-DAD analysis of flavonoids and hydroxycinnamic acids in Aster novi-belgii L.

Aster novi-belgii is a perennial ornamental herb native to eastern Canada and the United States of America, cultivated in Ukraine. This species should be considered a possible source of phenolic compounds, principally hydroxycinnamic acids and flavonoids. Therefore, in this study, the aim was to determine these compounds in Aster novi-belgii by HPLC-DAD analysis, and validation of this chromatographic method and lay a scientific and technical basis for the utilization and development of the plant resources of the cultivated plants of the genus Aster. The HPLC-DAD method determined the flavonoids and hydroxycinnamic acids composition and content in the herb of Aster novi-belgii L. The HPLC-DAD method allowed the detection of 13 phenolic compounds, namely 6 hydroxycinnamic acids (chlorogenic, sinapic, caffeic, syringic, trans-cinnamic, trans-ferulic acids), and 7 flavonoids (kaempferol 3-O-beta-D-glucoside, naringin, quercetin, luteolin, rutin, kaempferol, rhamnetin). The quantitative detection showed that the main hydroxycinnamic acids were chlorogenic acids (15069.21 ± 0.34 µg/g) and sinapic acids (949.95 ±0.22 µg/g). Concerning flavonoids, the largest amounts were kaempferol 3-O-beta-D-glucoside (8989.79 ±0.31 µg/g) and naringin (2092.02 ± 0.26 µg/g). HPLC-DAD method was evaluated in terms of linearity, precision, accuracy, limits of quantification, and limits of detection. The calibration curves of reference substances were linear (R2 ≥ 0.997), the LODs were in the range of 0.21–1.71 µg/mL, and the LOQs – of 0.48–5.19 µg/mL, respectively. Our phytochemical research confirms that the study material is a rich source of hydroxycinnamic acids and flavonoids. Findings mean that Aster novi-belgii is a promising plant because of the important role of these phenolic compounds in many biological processes

HPLC-DAD analysis of flavonoids and hydroxycinnamic acids in Aster novi-belgii L.

Aster novi-belgii is a perennial ornamental herb native to eastern Canada and the United States of America, cultivated in Ukraine. This species should be considered a possible source of phenolic compounds, principally hydroxycinnamic acids and flavonoids. Therefore, in this study, the aim was to determine these compounds in Aster novi-belgii by HPLC-DAD analysis, and validation of this chromatographic method and lay a scientific and technical basis for the utilization and development of the plant resources of the cultivated plants of the genus Aster. The HPLC-DAD method determined the flavonoids and hydroxycinnamic acids composition and content in the herb of Aster novi-belgii L. The HPLC-DAD method allowed the detection of 13 phenolic compounds, namely 6 hydroxycinnamic acids (chlorogenic, sinapic, caffeic, syringic, trans-cinnamic, trans-ferulic acids), and 7 flavonoids (kaempferol 3-O-beta-D-glucoside, naringin, quercetin, luteolin, rutin, kaempferol, rhamnetin). The quantitative detection showed that the main hydroxycinnamic acids were chlorogenic acids (15069.21 ± 0.34 µg/g) and sinapic acids (949.95 ±0.22 µg/g). Concerning flavonoids, the largest amounts were kaempferol 3-O-beta-D-glucoside (8989.79 ±0.31 µg/g) and naringin (2092.02 ± 0.26 µg/g). HPLC-DAD method was evaluated in terms of linearity, precision, accuracy, limits of quantification, and limits of detection. The calibration curves of reference substances were linear (R2 ≥ 0.997), the LODs were in the range of 0.21–1.71 µg/mL, and the LOQs – of 0.48–5.19 µg/mL, respectively. Our phytochemical research confirms that the study material is a rich source of hydroxycinnamic acids and flavonoids. Findings mean that Aster novi-belgii is a promising plant because of the important role of these phenolic compounds in many biological processes

Assessment of the State of the Epidemiological Situation of Lymphogranulomatosis in Different Regions of Ukraine as an Important Component in the Development of Directions for Improvement of Pharmaceutical Support of Oncohematological Patients

The aim: to conduct epidemiological studies of the morbidity and mortality of the population from lymphogranulomatosis in the regions of the country in dynamics over the years and to identify regions of the country that require special attention in the process of early detection of this pathology and effective pharmaceutical provision of patients.Materials and methods. The object of the research was the official data of the National Cancer Registry from 2012-2019. The research used both general theoretical (historical, analytical and comparative, systemic, graphic, logical, hypothetical-deductive) and applied (mathematical-statistical, epidemiological) methods of research.Results. It has been established that the average morbidity and mortality rates of the population from lymphogranulomatosis in Ukraine exceed similar world data (morbidity/mortality – 2.3/0.4 for men and 1.9/0.3 for women per 100 thousand population). Thus, the calculated morbidity rates were 2.5, and the mortality rate was 0.7 per 100 thousand population. It has been proved that morbidity and mortality rates by region varied in a significant range of values, namely, morbidity – from 1.8 (Transcarpathian region) to 3.0 (Kyiv), and mortality – from 0.4 (Cherkasy region) to 0.9 (Ivano-Frankivsk, Kyiv, Chernivtsi regions) per 100 thousand population. According to the results of the grouping, most regions of the country (14 regions and Kyiv city) were assigned to the group of extremely unfavourable (group D) development of the epidemiological situation in terms of lymphogranulomatosis. Ivano-Frankivsk region was attributed to the group of unfavourable (group C) development of this process. Four (Transcarpathian, Vinnytsia, Zhytomyr and Kherson) regions were included in the group of favourable (group A) development of the epidemiological situation. The group of relatively favourable (group B) – three areas. These are the Volyn, Chernigov and Cherkasy regions.Conclusions. The presence of an extremely unfavourable (14 regions and Kyiv city) and unfavourable (Ivano-Frankivsk region) onco-epidemiological situation necessitates the development and implementation of regional programs for the early detection of lymphogranulomatosis and the provision of patients with affordable and effective anticancer drugs in accordance with the existing resource provision at the level of regions and local communitie

Assessment of the State of the Epidemiological Situation of Lymphogranulomatosis in Different Regions of Ukraine as an Important Component in the Development of Directions for Improvement of Pharmaceutical Support of Oncohematological Patients

The aim: to conduct epidemiological studies of the morbidity and mortality of the population from lymphogranulomatosis in the regions of the country in dynamics over the years and to identify regions of the country that require special attention in the process of early detection of this pathology and effective pharmaceutical provision of patients.Materials and methods. The object of the research was the official data of the National Cancer Registry from 2012-2019. The research used both general theoretical (historical, analytical and comparative, systemic, graphic, logical, hypothetical-deductive) and applied (mathematical-statistical, epidemiological) methods of research.Results. It has been established that the average morbidity and mortality rates of the population from lymphogranulomatosis in Ukraine exceed similar world data (morbidity/mortality – 2.3/0.4 for men and 1.9/0.3 for women per 100 thousand population). Thus, the calculated morbidity rates were 2.5, and the mortality rate was 0.7 per 100 thousand population. It has been proved that morbidity and mortality rates by region varied in a significant range of values, namely, morbidity – from 1.8 (Transcarpathian region) to 3.0 (Kyiv), and mortality – from 0.4 (Cherkasy region) to 0.9 (Ivano-Frankivsk, Kyiv, Chernivtsi regions) per 100 thousand population. According to the results of the grouping, most regions of the country (14 regions and Kyiv city) were assigned to the group of extremely unfavourable (group D) development of the epidemiological situation in terms of lymphogranulomatosis. Ivano-Frankivsk region was attributed to the group of unfavourable (group C) development of this process. Four (Transcarpathian, Vinnytsia, Zhytomyr and Kherson) regions were included in the group of favourable (group A) development of the epidemiological situation. The group of relatively favourable (group B) – three areas. These are the Volyn, Chernigov and Cherkasy regions.Conclusions. The presence of an extremely unfavourable (14 regions and Kyiv city) and unfavourable (Ivano-Frankivsk region) onco-epidemiological situation necessitates the development and implementation of regional programs for the early detection of lymphogranulomatosis and the provision of patients with affordable and effective anticancer drugs in accordance with the existing resource provision at the level of regions and local communitie