15 research outputs found
Localization of Glycosyl Enzymes and Nucleotide-Sugar Transporters in the Endoplasmic Reticulum and the Golgi Apparatus
Epitope mapping of human herpesvirus-7 gp65 using monoclonal antibodies.
Human herpesvirus (HHV)-7 encodes a unique 65-kDa heparin-binding glycoprotein,
designated gp65. This molecule is thought to play a role in virus attachment and
entry. To obtain reagents to map the structure and function of HHV-7 gp65, we
produced monoclonal antibodies to this molecule. Ten monoclonal antibodies
reacting with gp65 on ELISA were subdivided in four groups on the basis of their
isotype and differential reactivity with (i) native versus denatured forms of
gp65, and (ii) mature (virion-associated) versus immature (cell-associated) forms
of the molecule. We were able to map the binding epitopes for eight of these ten
antibodies, and these were found to cluster to one site on gp65 (amino acids
239-278); within this region, the antibodies reacted with at least three distinct
domains (244-251, 255-262, 263-278). The reasons for the apparent immunodominance
of this region are uncertain. Taken together, this panel of antibodies
constitutes an extensive and well-characterized set of HHV-7 specific antibodies
that may have utility for future analyses of the structure/function of gp65, and
for studies on the virus life cycl
The Genomic Structure of the Human Charcot–Leyden Crystal Protein Gene Is Analogous to Those of the Galectin Genes
Effect of recombinant galectin-1 on the growth of immortal rat chondrocyte on chitosan-coated PLGA scaffold
Synthesis of an endogeneous lectin, galectin-1, by human endothelial cells is up-regulated by endothelial cell activation
Kinetic Modeling Confirms the Biosynthesis of Mucin Core 1 (Beta-Gal(1-3) Alpha-GalNAc-O-Ser/Thr) O-glycan Structures are Modulated by Neighboring Glycosylation Effects
Molecular mimicry in pauci-immune focal necrotizing glomerulonephritis
Pauci-immune focal necrotizing glomerulonephritis (FNGN) is a severe inflammatory disease associated with autoantibodies to neutrophil cytoplasmic antigens (ANCA). Here we characterize autoantibodies to lysosomal membrane protein-2 (LAMP-2) and show that they are a new ANCA subtype present in almost all individuals with FNGN. Consequently, its prevalence is nearly twice that of the classical ANCAs that recognize myeloperoxidase or proteinase-3. Furthermore, antibodies to LAMP-2 cause pauci-immune FNGN when injected into rats, and a monoclonal antibody to human LAMP-2 (H4B4) induces apoptosis of human microvascular endothelium in vitro. The autoantibodies in individuals with pauci-immune FNGN commonly recognize a human LAMP-2 epitope (designated P(41–49)) with 100% homology to the bacterial adhesin FimH, with which they cross-react. Rats immunized with FimH develop pauci-immune FNGN and also develop antibodies to rat and human LAMP-2. Finally, we show that infections with fimbriated pathogens are common before the onset of FNGN. Thus, FimH-triggered autoimmunity to LAMP-2 provides a previously undescribed clinically relevant molecular mechanism for the development of pauci-immune FNGN