35 research outputs found

    Incidence and possible sources of Tomato spotted wilt virus in tobacco grown in Denizli Province, Turkey

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    Tomato spotted wilt virus (TSWV) is economically prominent disease for its impact on tobacco {Nicotiana tabacum L.) production worldwide. An increase of the incidence of symptoms typical ofTSWV has been observed in tobacco production areas in Denizli province of Turkey where tobacco is significantly grown. Surveys were conducted to determine the prevalence status of TSWV in tobacco cultivars and its possible sources of infections in four tobacco growing districts of Denizli province. A total of 501 plant samples from field-grown tobaccos, weeds, potential intermediate hosts, seedlings and seeds were collected during 2019 and tested by DAS-ELISA. Of these plants, 243 belong to 55 dififerent weed species from 26 dififerent families with intermediate host potential. Throughout the study, 40 crop plant samples which could be intermediate hosts and 39 tobacco seed samples were also taken for testing. Adult thrips specimens were picked up from the fields and brought to the laboratory for preparations. Four vector virus species were detected when adult thrips individuals were diagnosed: Thrips tabaci Lindeman (Thysanoptera: Thripidae), Franklimella occidentals (Pergande) (Thysanoptera: Thripidae), Aeolothrips intermedius Bagnall (Thysanoptera: Aeolothripidae) and Thrips major Uzel (Thysanoptera: Thripidae). Of the 179 tobaccos sampled, 31.2% was positive; besides, of 243 weeds tested 10 were found to be infected. Echinochloa crus-galli and Tordylium apulum were determined to be new host recordings for TSWV infection. Only one tomato plant from the crop plants as intermediate hosts was infected. Cucumber mosaic virus (CMV), Alfalfa mosaic virus (AMV) and Potato virus Y (PVY) was also confirmed in tobacco fields © Articles by the authors; UASVM, Cluj-Napoca, Romania. The journal allows the author(s) to hold the copyright/to retain publishing rights without restrictionThis work was supported by Aegean Exporter's Association

    Determination of Physiological and Harvest Antioxidant Activities of Sunflower Plants Growing in Alkaline Soils with DPPH Method

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    Various methods are employed to evaluate the antioxidant capacities of herbal products and foods. In this study, sunflower widely cultivated in Tekirdag region, Tunca MR(5580) sunflower variety grown in the alkaline soils of the Banarli Village in the Hayrabolu County and antioxidant activities of sunflower plant seeds during physiological and harvest periods were researched. It was compared with Butylated Hydroxy Toluene (BHT), a synthetic antioxidant, which is widely used in the food industry. Antioxidant activity was determined by DPPH method. Free radical scavenging activity of methanol extracts of sunflower was measured by 1,1-diphenyl-2-picryl-hydrazyl using the method. All analysis was run in triplicate and averaged. Physiological period of the examples in the antioxidant activity of 28 %, while in the period of harvest activity as 25 %.Namik Kemal University Scientific Research UnitNamik Kemal University [NKUBAP.00.M6.AR.11.01]The project with the number NKUBAP.00.M6.AR.11.01 titled as Research of Antioxidant Capacity of Sunflower (Helianthus Annuus) Seeds Cultivated in Tekirdag Region has been funded by Namik Kemal University Scientific Research Unit

    Response of the cherry rootstock to water stress induced in vitro

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    The in vitro response of sweet cherry (Prunus cerasus × P. canescens) rootstock Gisela 5 to increasing water deficit in the culture medium was studied. Water stress induced by the incorporation of 1, 2 and 4 % polyethylene glycol (PEG-8000) into the Murashige and Skoog medium was applied for 6 weeks. PEG-induced water stress reduced shoot dry mass, length, water content and relative chlorophyll content. Water stress also induced leaf necrosis without causing loss of viability in the explants. The increase in malondialdehyde content indicated oxidative stress. The activities of antioxidant enzymes, such as superoxide dismutase (SOD), catalase (CAT), ascorbate peroxidase (APX), peroxidase (POX) and glutathione reductase (GR) were also significantly elevated. The concentrations of K, Ca, Fe and Mn of shoots were decreased. © 2008 Springer Science+Business Media B.V

    Responses of the cherry rootstock to salinity in vitro

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    WOS: 000247412400037The in vitro response of sweet cherry rootstock Gisela 5 (Prunus cerasus x Prunus canescens) to increasing concentrations of NaCl (0, 50, 100 and 150 mM) in the Murashige and Skoog culture medium was studied. Induced salinity reduced growth and chlorophyll content in shoots but had no effect on water content. The increase in malondialdehyde content indicated that salinity induced oxidative stress which was accompanied with the visible symptoms of salt injury in the shoots. Antioxidant enzymes, such as superoxide dismutase, ascorbate peroxidase, peroxidase, catalase, and glutathione reductase were also significantly elevated. Although no change was observed in the Cl concentration, Na concentration of shoots significantly rose and NaCl treatments impaired K, Ca and Mg nutrition and induced imbalance in K:Na and Na:Ca ratios
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