296 research outputs found
Simultaneous measurement of MMP9 and TIMP1 mRNA in human non small cell lung cancers by multiplex real time RT-PCR.
Influence of 17q gain and promoter polymorphisms on mRNA expression of somatostatin receptor type 2 in neuroblastoma.
Genetic variants in miR-146a, miR-149, miR-196a2, miR-499 and their influence on relative expression in lung cancers
Genetic variants in miR-146a, miR-149, miR-196a2, miR-499 and their influence on relative expression in lung cancers.
High-resolution melting analysis for rapid detection of KRAS, BRAF, and PIK3CA gene mutations in colorectal cancer.
Idatidosi Splenica. Descrizione di un caso clinico e revisione della letteratura
A rare case of primary hydatid cyst of the spleen is described. The hypotheses of this possible primary localization, diagnostic techniques and the therapeutic management are discussed
SPIDIA-DNA: an External Quality Assessment for the pre-analytical phase of blood samples used for DNA-based analyses.
Mutational analysis of single circulating tumor cells by next generation sequencing in metastatic breast cancer
High-resolution melting analysis : a new molecular approach for the early detection of Diplodia pinea in Austrian pine
The differentiation of Diplodia pinea from closely related species, such as Diplodia scrobiculata
and Diplodia seriata, and its detection in plant tissue, represented a critical issue for
a long time. Molecular screening tools have recently been developed to address this topic.
In this study we applied one of the most sensitive and rapid diagnostic screening method
so far developed, called High-Resolution Melting Analysis (HRMA), to detect D. pinea in Austrian
pine (Pinus nigra). HRMA exploits differences in the melting behaviour of PCR products
to rapidly identify DNA sequence variants without the need for cumbersome post-PCR
methods. We developed a HRMA method to detect specific fungal sequences in the mitochondrial
small subunit ribosome gene (mt SSU rDNA). The reliability of this technique
was firstly assessed on DNA extracted from pure cultures of D. pinea and closely related
species. Amplicon differences were screened by HRMA and the results confirmed by direct
DNA sequencing. Subsequently, HRMA was tested on DNA from symptomatic and symptomless
pine shoots, and the presence of the fungus was also confirmed by both conventional
and molecular quantitative approaches. The HRMA allowed the distinction of
D. pinea from closely related species, showing specific melting profiles for the each pathogen.
This new molecular technique, here tested in a plantefungus pathosystem for the first
time, was very reliable in both symptomatic and symptomless shoots. HRMA is therefore
a highly effective and accurate technique that permits the rapid screening of pathogens
in the host.This work was
supported by grant to Nicola Luchi from the Consorzio Interuniversitario Biotecnologie (CIB, Italy).http://www.elsevier.com/locate/funbionf201
SPIDIA-RNA: second external quality assessment for the pre-analytical phase of blood samples used for RNA based analyses.
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