A new concept of automated manufacturing process for wire rope terminals

30th International Conference on Flexible Automation and Intelligent Manufacturing (FAIM2021) 15-18 June 2021, Athens, Greece.The products related to automotive industry need to be extremely competitive. The metallic wire ropes used to open doors in the vehicles are based on a braided wire rope provided with accessories. The more expensive is the vehicle, the more complex is the required final wire rope. In order to prevent the corrosion and reduce noise, the internal metallic cable can be coated with a polymer via extrusion. However, this coating needs to be removed from the extremities of the metallic cable, allowing the zamak injection of the mechanical terminals, preventing die casting defects. Nevertheless, the standard machines able to produce the mechanical terminals by die casting process were always prepared to work only with non-coated metallic cable. Customers demanding coated metallic cables impose the need to adapt the standard machines to this new reality. Thus, new systems needed to be added to the standard machine, allowing the metallic cable strip operation. Moreover, this operation needs to be added to the process but without repercussions on the cycle time. Different approaches were studied and tested in order to find the best solution. The new device was designed and produced, allowing its test in real conditions. The system based on just one blade did not show satisfactory results, being necessary the use of a system based on multiple blades. The new device, when connected to the standard machine, allows to produce wire ropes up to 2.5 meters long, striped in both sides, letting the die casting injection of the zamak terminal in one extremity, with a cycle time of 7 seconds (1000 wire ropes per hour, as they are produced in couples).The authors would like to thank Mr. Mário Cardoso for his support and commitment with this work, providing all data necessary to carry out this development. The authors would like to acknowledge financial support from Fundação para a Ciência e Tecnologia (grants: UID/EMS/00667/2019 and UID/UIDB/CPO/04058/2020info:eu-repo/semantics/publishedVersio

Implementing TPM supported by 5S to improve the availability of an automotive production line

The maintenance management is a topic of strategic importance for automotive manufacturers. In fact, an effective maintenance process and a preventive maintenance (PM) procedure can significantly reduce the risk of equipment failures that can lead to downtime on the production lines. However, due to the complexity of an automotive production system, the risk of failure on a crucial piece of the production equipment cannot be entirely avoided. The study made in this paper aims at improving the availability of a critical production line through the total productive maintenance (TPM) methodology and supported by Lean Maintenance tools. An analysis is made of the initial condition of the line where the main problems are identified by employing several tools for this purpose, such as Mean Time between Failures (MTBF), Mean Time to Repair (MTTR), Overall Equipment Efficiency (OEE) and Availability (A). In response to the identified problems, an action plan is developed and implemented in order to find the root cause of the high number of malfunctions and faults in one of the line’s equipment with the use of 5S tools, visual management, and maintenance progress, as well as the development of a training program to increase operators’ skills. The results of such actions were positive as the line became more organized, the value of the MTBF increased, the MTTR value decreased, and consequently the overall availability increased.info:eu-repo/semantics/publishedVersio

Anadenanthera colubrina vell brenan : anti-candida and antibiofilm activities, toxicity and therapeutical action

We evaluated the antifungal and antibiofilm potential of the hydroalcoholic extract of bark from Anadenanthera colubrina (vell.) Brenan, known as Angico, against Candida spp. Antifungal activity was evaluated using the microdilution technique through the Minimum Inhibitory and Fungicide Concentrations (MIC and MFC). The antibiofilm potential was tested in mature biofilms formed by Candida species and analyzed through the counting of CFU/mL and scanning electron micrograph (SEM). In vivo toxicity and therapeutic action was evaluated in the Galleria mellonella model. The treatment with the extract, in low doses, was able to reduce the growth of planktonic cells of Candida species. MIC values range between 19.5 and 39 µg/mL and MFC values range between 79 and 625 µg/mL. In addition was able to reduce the number of CFU/mL in biofilms and to cause structural alteration and cellular destruction, observed via SEM. A. colubrina showed low toxicity in the in vivo assay, having not affected the viability of the larvae at doses below 100mg/kg and high potential in the treatment of C. albicans infection. Considering its high antifungal potential, its low toxicity and potential to treatment of infections in in vivo model, A. colubrina extract is a strong candidate for development of a new agent for the treatment of oral candidiasis33CONSELHO NACIONAL DE DESENVOLVIMENTO CIENTÍFICO E TECNOLÓGICO - CNPQ552562/2011-

Características do sêmen a fresco e descongelado de garanhões da raça Nordestina

RESUMO: Este estudo descreveu as características seminais, da membrana plasmática e do acrossoma de espermatozoide congelado/descongelado de 19 ejaculados de garanhões da raça Nordestina. Os aspectos analisados incluíram os parâmetros físicos do sêmen fresco; a motilidade e a longevidade do sêmen diluído e descongelado; a morfologia espermática, integridade funcional e estrutural da membrana plasmática do espermatozoide e a habilidade de ligação do espermatozoide à membrana perivitelina da gema do ovo de galinha do sêmen descongelado. As variáveis foram avaliadas pela ANOVA com post hoc teste de Student Newman-Keuls (P<0,05). A MT e a MP foram maiores (P<0,05) no sêmen diluído do que no descongelado. A percentagem média de defeitos maiores, menores e totais foi muito inferior ao limite recomendado pelo CBRA. A porcentagem de reativos ao HOST foi de 14,21±1,12% e a porcentagem média de membranas íntegras detectadas pelo teste supravital de 62,22±9,06% e pela sonda SYBR-14 de 81,47±26,90. O número médio de espermatozoides ligados à MPV após a descongelação do sêmen foi de 230,39±57,09. A MT e MP no tempo 0 min do TTR foi superior (P<0,05) em relação a 150 min, não diferindo nos tempos 10 min e 30 min. Os resultados demonstram que a utilização dos testes laboratoriais adicionais ajudam no processo de avaliação das amostras, possibilitando a obtenção de informações mais confiáveis e precisas. Embora a criopreservação tenha provocado queda na motilidade seminal, o uso de diluidor contendo amidas minimizou os danos osmóticos nas células espermáticas e manteve a integridade morfológica, funcional e estrutural da membrana plasmática do espermatozoide. Estes resultados são um referencial em estudos futuros uma vez que, inexistem dados comparativos nesta raça