Molecular cloning and characterization of ABCG/PDR-type ABC transporter in grape berry skin

Grape (Vitis vinifera L.) skin contains the phenolic compound resveratrol which is important not only for resistance to biotic and abiotic stresses but also for human health. However, little is known about resveratrol transport in plant cells. ABC (ATP binding cassette) transporters are well-known transporters responsible for secondary metabolite accumulation in plants. Previous reports speculated that the full-size ABCG transporter pleiotropic drug-resistant (PDR) is involved in resveratrol transport in fungi and plants. In this paper, all full-size ABCG transporters found in the grape genome database are listed and focus is placed on VvABCG44/VvPDR14 as a candidate resveratrol transporter. The full-length cDNA of VvABCG44 was cloned by RT-PCR using mRNAs extracted from grape berry skin. VvABCG44 expression was induced by UV irradiation, and the expression pattern of VvABCG44 in various grape organs was similar to that of stilbene synthase (STS), a key enzyme in resveratrol synthesis. Resveratrol content in grape berry skin increased after UV irradiation. These results suggest that VvABCG44 functions as a resveratrol transporter in grape

Genotype and Growing Environment Interaction Shows a Positive Correlation between Substrates of Raffinose Family Oligosaccharides (RFO) Biosynthesis and Their Accumulation in Chickpea (Cicer arietinum L.) Seeds

To develop genetic improvement strategies to modulate raffinose family oligosaccharides (RFO) concentration in chickpea (Cicer arietinum L.) seeds, RFO and their precursor concentrations were analyzed in 171 chickpea genotypes from diverse geographical origins. The genotypes were grown in replicated trials over two years in the field (Patancheru, India) and in the greenhouse (Saskatoon, Canada). Analysis of variance revealed a significant impact of genotype, environment, and their interaction on RFO concentration in chickpea seeds. Total RFO concentration ranged from 1.58 to 5.31 mmol/100 g and from 2.11 to 5.83 mmol/100 g in desi and kabuli genotypes, respectively. Sucrose (0.60−3.59 g/100 g) and stachyose (0.18−2.38 g/ 100 g) were distinguished as the major soluble sugar and RFO, respectively. Correlation analysis revealed a significant positive correlation between substrate and product concentration in RFO biosynthesis. In chickpea seeds, raffinose, stachyose, and verbascose showed a moderate broad sense heritability (0.25−0.56), suggesting the use of a multilocation trials based approach in chickpea seed quality improvement programs