Study of growth, survival rate and final harvesting of Litopenaeus vannamei impact of 25, 35 and 50 (per/m^2) stocking densities

For the first time in research program and species diversity the white legged shrimp (Litopenaeus vannamei) was introduced in Iran in 2005 by Iranian Fisheries Research Organization. In two recent years two projects was carried out successfully in Hele Research Station and according of these projects study of growth, survival rate and final harvesting of Litopenaeus vannamei impact of 25, 35 and 50 (per/m^2) stocking densities was done .Nine 0.4 ha earthen ponds were stocked from 26 June 2006 to 11 November 2006 and the rate of stocking was 100000,140000 and 200000 Pl in 25, 35 and 50 per /m^2 respectively. We also carried out daily test of pH, temperature and dissolve oxygen two times per day but transparency, depth and salinity one time per day. The mean weight in 120 days was 17/84±2/6, 16/03±1/9 and14/09±2/3 grams in 25, 35 and 50 per /m^2 respectively and highest weight was seen in 25 density. Results demonstrated that there was a significant relation (P<0.05) between mean weight. The yield were 1745, 2055 and 2425 kg/pond although 4/3, 5/1 and 6 ton/ha in 25, 35 and 50 per /m^3 respectively. The maximum and minimum survival rate 97/96 and 88/46 was seen in 25 and 50 per/m^2. The minimum FCR 1.43 was seen in 25 per /m^2. For economical calculating just by using the cost of larva and feeding indicates that the highest benefit percentage was seen in 25 density. The average of salinity, pH, temperature and dissolve oxygen were 36.1-37 ppt, 8.1, 30.2-30.6 degrees centigrade and 5.9- 6.4 mg/l

Development gene data bank of cultured shrimp species in Iran

DNA barcode is a short, standard well known sequence of cytochrome oxidase І gene. By using this DNA sequence can be realized that each animal, plant or fungus belongs to which species. in this research, samples were collected from imported cultured shrimp Litopenaeus vannamei and and 6 Persian Gulf and Oman Sea shrimp species which classified based on traditional systematically as: Penaeus semisulcatus, Fenneropenaeus merguiensis, Metapenaeus affinis, Parapenaeopsis Stylifera and Fenneropenaeus indicus. After examination of DNA barcode sequence, molecular and bioinformatics operations of each sequence in the Consortium for the Barcode of Life (CBOL) and National Center for Biotechnology Information (NCBI), phylogenetic analysis of each sample was determined and similarity of each sample with NCBI and CBOL database was checked and the closest species to each sample were specified. According to the results different samples of L. vannamei, . banded P.semisulcatus, F. merguiensis and F. indicus have more than 97% similarity to the same species of other countries. non banded P.semisulcatus had 80.07% similarity to banded P.semisulcatus, M. affinis samples had 90.3% similarity to Metapenaeus ensis and Parap. Stylifera had 93.44% similarity to Parapenaeopsis coromandelica in the CBOL. This funding confirmed the need for further investigation and possible announcement of new species

Lack of effect of adenosine on the function of rodent osteoblasts and osteoclasts in vitro

Extracellular ATP, signalling through P2 receptors, exerts well-documented effects on bone cells, inhibiting mineral deposition by osteoblasts and stimulating the formation and resorptive activity of osteoclasts. The aims of this study were to determine the potential osteotropic effects of adenosine, the hydrolysis product of ATP, on primary bone cells in vitro. We determined the effect of exogenous adenosine on (1) the growth, alkaline phosphatase (TNAP) activity and bone-forming ability of osteoblasts derived from the calvariae of neonatal rats and mice and the marrow of juvenile rats and (2) the formation and resorptive activity of osteoclasts from juvenile mouse marrow. Reverse transcription polymerase chain reaction (RT-PCR) analysis showed marked differences in the expression of P1 receptors in osteoblasts from different sources. Whilst mRNA for the A1 and A2B receptors was expressed by all primary osteoblasts, A2A receptor expression was limited to rat bone marrow and mouse calvarial osteoblasts and the A3 receptor to rat bone marrow osteoblasts. We found that adenosine had no detectable effects on cell growth, TNAP activity or bone formation by rodent osteoblasts in vitro. The analogue 2-chloroadenosine, which is hydrolysed more slowly than adenosine, had no effects on rat or mouse calvarial osteoblasts but increased TNAP activity and bone formation by rat bone marrow osteoblasts by 30–50 % at a concentration of 1 μM. Osteoclasts were found to express the A2A, A2B and A3 receptors; however, neither adenosine (≤100 μM) nor 2-chloroadenosine (≤10 μM) had any effect on the formation or resorptive activity of mouse osteoclasts in vitro. These results suggest that adenosine, unlike ATP, is not a major signalling molecule in the bone

Principal Components Analysis of Main Factors Affecting Noise Pollution in Main Road of Golestan National Park

Traffic noise pollution is a serious threat to wildlife species, resulting in isolating and reducing the population. The Asian Highway of Tehran-Mashhad, from which a 26.51 km long part is located inside Golestan National Park, is the main source of noise disturbance inside the area. The purpose of this study was to determine the most important criteria for noise pollution of this road; in order to achieve this goal, the Principle Components Analysis method was used. To assess the sound emissions and to study the parameters affecting it, desk studies and field visits were used in a 250-meter buffer zone. A total of 16 variables in three different categories of traffic parameters, geometry parameters and environmental parameters were extracted, andthe circular plots were measured at a radius of 25 m &nbsp;for 15 minutes. The results of PCA showed that six main factors including the speed of light vehicles, the humidity,&nbsp; the altitude above sea level, the number of light vehicles, the distance from the road and the plot slope could justify a total of 72.396 % of the variance of the data. Based on the results, management measures could&nbsp; be taken to reduce the noise pollution based on the extracted criteria, and the noise pollution could be managed more efficiently by spending less time and money. &nbsp; &nbsp

Evaluation of Genetic Variation of Yellow and White Astragals (Astragalus sp.) in Protected Regions of Isfahan Province Using ISSR Marker

Genetic variation of 16 white and yellow astragal accessions collected from three protected regions of Isfahan province (Mooteh, Kolah-Ghazi and Ghamishloo) were evaluated using ISSR marker. Nine ISSR primers produced 221 bands in which 204 were polymorphic among astragal accessions. ISSR banding patterns were transformed into binary data of presence–absence and matrices were processed with NTSYS Ver. 2 software. Mantel correspondence tests were conducted to select the best similarity coefficient. Dendrogram was constructed using the simple matching coefficient with unweighted pair group mathematical average (UPGMA). Cluster analysis showed the presence of two large and five small groups and the PCoA data confirmed the results of clustering. Results showed that white astragals have more genetic similarity but yellow astragals have more genetic diversity and it is necessary to improve their systematic identification. The highest gene diversity was observed in Kolah-Ghazi (0.366) and the least gene diversity belonged to Moote (0.129). So, the regions with low gene diversity (e. g. Mooteh) should be more protected in relation to astragal accessions and it is necessary to apply genetic conservation strategies in these regions

An Epidemiological Study of Accidents in a Construction Industry: A Case-Control Study

Background and Objectives: Despite science and technology development and their application in various industries, accidents continue to occur in many workplaces. This study was designed and carried out with the aim of epidemiological survey of accident among workers of a tunneling industry. Methods: This study was conducted on 144 out of a total of 440 employees of a tunneling project who were at risk of occupational accidents. 48 workers with a history of occupational accident over the past two years were selected as case group and 96 workers of the same project were selected as control group. The data were collected through a designed checklist and analyzed using logistic regression, chi-square and independent t tests. Results: A significant association was observed between accident rate and different age groups (p<0.05). There was a significant relationship (p=0.016) in the case of sport activities between case and control groups, and significant relationships were observed between two groups regarding education (p=0.057) and smoking (p=0.06), but there was no significant relationships between accident occurrence with marital status, residence in workplace, job related education, job experience, chronic diseases and obesity. Conclusion: The results of this study clarify the necessity of the use of epidemiological data in preventive and control measures in workplaces. Therefore, Developing programs for determination of physical and mental capacity of workers are essential to employ them in jobs commensurate with their abilities, especially in older workers

Mesenchymal Stem Cells and Periodontal Regeneration

The use of mesenchymal stem cell (MSC) therapy offers the potential to develop a completely novel and improved method of periodontal regeneration compared to existing methods. Since the initial demonstration of the presence of MSCs in the periodontal ligament, many recent studies have now demonstrated the potential for the transplantation of MSCs from PDL and other sources to enhance periodontal regenerative outcomes in a variety of animal models. In addition, the recent demonstration of the possible utility of allogeneic MSCs and MSCs derived from inducible pluripotent stem cells may offer new methods of delivering such therapies. Determination of the specific roles that MSCs may play in enhancing regenerative outcomes requires further investigation. The principle of MSC-directed periodontal regenerative therapy is accepted in the field, but extensive investigation is necessary to establish viable, efficacious, and practically applicable human therapies.</p