Effect of Alcoholic Leaf Extract of Hellotropium Earopiam on the Levels of Testosterone , Prolactin ,TSH ,T3 and T4 Hormones in Blood of Male Albino Rats Exposed to Oxidative Stress by H2O2

The current study aimed to compare the effects of alcoholic leaf extract of Heliotropium europiam and Vit. C as antioxidants on levels of Testosterone (testo) , Prolactin(PRL) ,TSH ,T3,T4 hormones in blood of adult albino male rats exposed to oxidativ  stress by hydrogen peroxide (0.5%) in the drinking water throughout (30) days. For the purpose of the study preliminary experiments were carred out yo find the most effective dose of plant extracts and it was found that (300mg /  kg).. Rats (3-4 months)age and (250±285g) weights were distributed randomly into five groups each group included five rats as follows: control group, H2O2 group, H2O2+ Heliotropium europiam alcoholic leaf extract group, H2O2+ Vit. C group, H2O2+ Heliotropium europiam alcoholic leaf extract+Vit.C group. The plant extract of leaves as (300) mg / kg and Vit.C (250) mgkg of body weight in a single daily dose.The results of the statistical analysis shwed that the animals exposed to stress oxidative mediated by hydrogen peroxide led to a significant decrease (p ?0.05) in the levels of testo. and RRLand T3 and non significant difference in the  TSH and T4 levels when compared with control.  The treatment with H2O2+ plant extract shwed  asignificant increase in the testo. level and a significant decrease in T3 andT4 levels, and lack of significant difference in the levels of PRL and TSH, while the treatmen of group H2O2+Vit.C led to asignificant increase in testo., PRLand TSH and a significant decrease inT3 andT4.While the group exposed to stress and treated with plant extract and Vit.C showed asignificant increase in testo. And PRL levels and significant decrease in TSH and T4 with non significant difference in the T3 level when  comparedwith  hydrogen peroxide group. Keywords : Hellotropium earopiam, H2O2, Free racicals, oxidative stress Testosterone ,    Prolactin ,TSH

ANTIOXIDATIVE EFFECT OF HELIOTROPIUM EUROPIUM ALCOHOLIC LEAVES EXTRACT IN ALBINO MALE RATS EXPOSED TO OXIDATIVE STRESS.

The present study aimed to investigate someAntioxidant effects of alcoholic leaves extract of Heliotropium by measurement some antioxidant levels such as (glutathione and superoxide dismutase activity ),and the levels of Malondialdehyde (MDA), and peroxy nitrate radical( ONOO._ )and somebiochemical parameter such as total protein, albumin and urea in male rats exposed to oxidative stress by hydrogen peroxide (0.5% )in drinking water throughout the experimental period (30) days, and comparison these effects with the effects of the vitamin C as well known anti-oxidant . For purpose of this study preliminary experiments were carried out to find the most effective dose of Heliotropiumextract and was found (300) mg / kg of body weight. (35) male albino rats(4-5) months and( 245-280gm weight) were divided to (7) groups both Group included (5) rats as follows: The control group, hydrogen peroxide (H2O2 group,, Heliotropium extract group. H2O2+ Heliotropium extract group. (Heliotropium) Extract + Vitamin C group, .H2O2 + Vitamin C group. H2O2+ extract of Heliotropium + vitamin C group, The results of study showed that the oxidative stress by hydrogen peroxide led to a significant decrease in the concentration of glutathione activity of the enzyme SOD, total protein and albumin when compared with the control group. And a significant increase in the concentration of MDA, peroxynitrate radical and urea. Where the treatment of animals with Heliotropium + H2O2 led to a significant increase in the concentration of glutathione, SOD activity, total protein and albumin compared with a group of hydrogen peroxide. And a significant decrease in the concentration of MDA peroxy nitrite radical and urea compared with a group of hydrogen peroxide. The treatment of animals exposed to oxidative stress with vitamin C (250 mg / kg of body weight) for 30 days showed a significant increase in the concentration of glutathione enzyme SOD activity, total protein and albumin when compared with hydrogen peroxide. And a significant decrease in the concentration of MDA and peroxynitrite radical. And non-significant differences in the concentration of urea compared with a group of hydrogen peroxide. Well the treatment of animals with vitamin C and extract of Heliotropium has led to a significant increase in the concentration of glutathione SOD activity total protein and albumin when compared with a hydrogen peroxide group, and a significant decrease in the concentration of MDA, peroxy nitrite radicals and urea compared with hydrogen peroxide