Evaluation of Probiotic Potential of Some Native Lactobacillus Strains on the Growth Performance and Serum Biochemical Parameters of Japanese Quails (Coturnix Coturnix Japonica) during Rearing Period

ABSTRACT The objective of the present study was to evaluate the probiotic effects of different concentrations of four selected native Lactobacillus strains on the growth performance and serum biochemical parameters of Japanese quails. A completely randomized design (CRD) was applied, including seven probiotic treatments with four replicates of 20 quails each, totaling 560 quails. Treatments were applied for five weeks. Four native Lactobacillus strains were anaerobically grown in a 10-L batch fermenter and lyophilized (1010 CFU/g). Treatments were as follows: T1: control (basal diet); T2: commercial probiotic CP1; T3: commercial probiotic CP2; and T4, T5, T6, and T7: four native strains added at levels of 50, 100, 150, and 200 g/ton diet, respectively. The native probiotics significantly improved body weight gain (BWG) and feed conversion ratio (FCR) during the starter, finisher, and overall periods (35 days) (p0.05) on blood cholesterol, calcium, high-density lipoprotein (HDL), or hemoglobin (HB) levels or on red blood cell counts (RBC). The cecal and small intestine samples of the quails fed the native Lactobacillus strains contained significantly higher Lactobacillus spp. and lower E. coli populations compared with the control diet and those supplemented with commercial probiotics. It was concluded that the use of the native Lactobacillus strains (150 g/ton diet) promoted the best performance of Japanese quails

Determination Of Appropriate Antibiotic In Bacterial Meningitis Of Children Based On MIC

Background: Bacterial meningitis is one of the most serious infections in infants and children. Three organisms include S.Pneumo;N.mening;H.Influ are the most common cause of meningitis in children between 2M-14y age.Etest is a new method for determination the MIC of some antimicrobial drugs in agarose .This method is useful for some organisms like as S .Pneumo; N.mening; H.Influ;sensitive Streptococcus and anaerobic ;aerobic gram negative. Materials and Methods: In this descriptive cross sectional study; In 57 suspected meningitis children ; organisms isolated from blood; CSF or other sterile boy fluid after culturing and antibiogram;. .MIC of someorganisms detected by Etest method. Results: Streptococcuswas the most prevalent ( 70%) and S.pneumon( 90% of all Streptococcus); H.infl 2%;N.mening 4%and L.monocyt 6%(more than expected);Gram negative (Ecoli; Klebsiella; entrobacter and psudomona) 18%. There was significant difference (P =0.01)in type of organisms between age groups. S.pneumonia was more frequent in children > 2 year ;N.meningitis in>4yr old .Site of isolation :blood; CSF (35.8*;28.3%)other sterile site 18.4%;concomitant positive culture in two site:17%.Mean age in Streptococcus was significantly different with Listeria (p=0.05); N.meningitis (p=0.04);H.influ (p=0.04);.but no difference with Staphylococcus;Klebsiella and E.coli Two type of H.inf were sensitive to Ampici or chloram ;both of them were sensitive to ceftiaxon. GBS were sensitive to PNC or Ampici Strep.nonAnonBnon- Cotrimoxazol>32mic/ml /PNC >256mic/ml/ Vanco>256mic/ml Strep.D: Cotrimoxazol>0.062mg/ml/ /PNC >0.016mic/ml/Imipenem>0.032mic/ml. Strep Pneumonia: All fo them were sensitive except 3 cases /Cotrimoxazol>2ic/ml /PNC =0.01mic/ml/Vanco>0.125mic/ m Vanco>0. 25mic/ ml/.Cotrimoxazol>2ic/ml / PNC =0.01mg/ml Vanco>0.125mic/ ml / Cotrimoxazol>2mic/ml /MIC-PNC >0.016mic/ml Therefore high dose of PNC is adequate for S.pneu ;because of Interm resistance to PNC All 3 N.menin were sensitive to PNC;Chlora;Ceftria and vanco Resistant to all drugs and high MIC for cefotaxime;CIPRO>32mic/ml. E coli: Pseudomona Aerogenosa:: Ceftriaxon>256mic/ml/ /Genta>0.038mg/ml Imipenem>32mic/ml. Klebsiella; only Sensitive to Cipro Staph .Aureous:Sensitive to Clinda;Cipro;Chlora;Resistant toCeft;PNCand Cotri Conclusion: Most type of N.meningitidis;H.inf and S.pneumonia were sensitive to many drugs. Only minority of them were resistant to Ampicillin but sensitive to chloramphenicol and vice versa. limited number of pneumococcal resistance to penicillin is medium resistance( MIC:0.1-1) .we can treat this resistant type by increasing of penicillin dosage .The others were sensitive to all drugs. Therefore ampicillin and chloramphenicol are the drug of choice in empiric treatment of bacterial meningitis after neonatal period