16 research outputs found
Prospective evaluation of accuracy and clinical utility of the Dual Path Platform (DPP) assay for the point-of-care diagnosis of leptospirosis in hospitalized patients
Identification of immunodominant antigens in canine leptospirosis by Multi-Antigen Print ImmunoAssay (MAPIA)
One Health Interactions of Chagas Disease Vectors, Canid Hosts, and Human Residents along the Texas-Mexico Border
Characterization of Proteinuria in Dogue de Bordeaux Dogs, a Breed Predisposed to a Familial Glomerulonephropathy: A Retrospective Study
Severe leptospirosis in tropical Australia: Optimising intensive care unit management to reduce mortality
The salivary gland proteome of root-galling grape phylloxera (Daktulosphaira vitifoliae Fitch) feeding on Vitis spp.
The successful parasitisation of a plant by a phytophagous insect is dependent on the delivery
of effector molecules into the host. Sedentary gall forming insects, such as grape phylloxera
(Daktulosphaira vitifoliae FITCH, Phylloxeridae), secrete multiple effectors into host
plant tissues that alter or modulate the cellular and molecular environment to the benefit of
the insect. The identification and characterisation of effector proteins will provide insight into
the host-phylloxera interaction specifically the gall-induction processes and potential mechanisms
of plant resistance. Using proteomic mass spectrometry and in-silico secretory prediction,
420 putative effectors were determined from the salivary glands or the root-feeding
D. vitifoliae larvae reared on Teleki 5C (V. berlandieri x V. riparia). Among them, 170 conserved
effectors were shared between D. vitifoliae and fourteen phytophagous insect species.
Quantitative RT-PCR analysis of five conserved effector candidates (protein disulfideisomerase,
peroxidoredoxin, peroxidase and a carboxypeptidase) revealed that their gene
expression decreased, when larvae were starved for 24 h, supporting their assignment as
effector molecules. The D. vitifoliae effectors identified here represent a functionally diverse
group, comprising both conserved and unique proteins that provide new insight into the D.
vitifoliae–Vitis spp. interaction and the potential mechanisms by which D. vitifoliae establishes
the feeding site, suppresses plant defences and modulates nutrient uptake