12 research outputs found
Leucine-glycine and carnosine dipeptides prevent diabetes induced by multiple low-dose streptozotocin in experimental model of adult mice
Aims/Introduction
Peptides are considered as quasiâhormones and effective molecules for regulation of the cells function and metabolic disorders prevention. Diâ and tripeptides with the ability to gastrointestinal absorption have been proposed to prevent diabetes progression.
Materials and Methods
Small peptides with different sequences of specific amino acids were synthesized based on a solid phase peptide synthesis (SPPS) protocol as well as carnosine (A) and glutathione (B) were examined for the prevention of diabetes induced by multiple lowâdose of streptozotocin (MLDS) in mice.
Results
The peptides A, LeuâGly (D) and ProâPro (F) exhibited a preventive effects on blood glucose elevation and impairment of the signaling and performance of beta cells. The beta cells function assessed by immunofluorescence and blood glucose level in mice exposed to diabetes treated by the peptides A and D was similar to the normal mice. The peptide D prevented from body weight loss caused by diabetes induction. The use of D and A peptides dramatically prevents the incidence of disruption in beta cells signaling by maintaining the natural balance of intracellular Aktâ2 and cAMP.
Conclusions
The results proved that peptide D (LeuâGly) named Hannaneh inhibits the body weight loss caused by diabetes induction. The Hannaneh and carnosine dipeptides with preservation of normal beta cell signalling and anti DPPâ4 activity were prevented from increasing the blood glucose in mice at risk of diabetes. These dipeptides may be regarded as the pharmaceutical agents for the prevention of diabetes