28 research outputs found
A systematic review and diagnostic test accuracy meta-analysis of the validity of anion gap as a screening tool for hyperlactatemia
Point-of-care umbilical arterial lactate and newborn outcomes in a low resource setting: cohort study
The methionine synthase polymorphism D919G alters susceptibility to primary nervous system lymphoma
What do we know about the variability in survival of patients with amyotrophic lateral sclerosis?
The methionine synthase polymorphism D919G alters susceptibility to primary central nervous system lymphoma
Development and Characterization of a Porcine Mitral Valve Scaffold for Tissue Engineering
This paper is in closed access.Decellularized scaffolds represent a promising alternative for mitral valve (MV) replacement. This work developed and characterized a protocol for the decellularization of
whole MVs. Porcine MVs were decellularized with 0.5% (w/
v) SDS and 0.5% (w/v) SD and sterilized with 0.1% (v/v) PAA.
Decellularized samples were seeded with human foreskin fibroblasts and human adipose-derived stem cells to investigate
cellular repopulation and infiltration, and with human colonyforming endothelial cells to investigate collagen IV formation.
Histology revealed an acellular scaffold with a generally conserved histoarchitecture, but collagen IV loss. Following
decellularization, no significant changes were observed in
the hydroxyproline content, but there was a significant reduction in the glycosaminoglycan content. SEM/TEM analysis
confirmed cellular removal and loss of some extracellular matrix components. Collagen and elastin were generally
preserved. The endothelial cells produced newly formed collagen IVon the non-cytotoxic scaffold. The protocol produced
acellular scaffolds with generally preserved histoarchitecture,
biochemistry, and biomechanics