Equipment and methods of microbiological sampling from deep levels of ice in central Antarctica

We report on the equipment, methods and results of microbiological sampling from deep levels of ice in Central Antarctica. The equipment includes a special microbiological drill laboratory (MBU) thermodrill (TELGA 14M), unit for sterile sampring from the ice core (USL) and borehole sampling unit (PSM-152). We report the methods of sterile sampling of ice core with USL-unit and results of investigations of ice core from deep levels of ice on Vostok Station, accomplished in Institute of Microbiology Russian Academy of Science (RAN) and new borehole sterile sampling with PSM-152-unit carried out in 1991 on the Vostok Station

Abundance, viability and diversity of the indigenous microbial populations at different depths of the NEEM Greenland ice core

The 2537-m-deep North Greenland Eemian Ice Drilling (NEEM) core provided a first-time opportunity to perform extensive microbiological analyses on selected, recently drilled ice core samples representing different depths, ages, ice structures, deposition climates and ionic compositions. Here, we applied cultivation, small subunit (SSU) rRNA gene clone library construction and Illumina next-generation sequencing (NGS) targeting the V4–V5 region, to examine the microbial abundance, viability and diversity in five decontaminated NEEM samples from selected depths (101.2, 633.05, 643.5, 1729.75 and 2051.5 m) deposited 300–80 000 years ago. These comparisons of the indigenous glacial microbial populations in the ice samples detected significant spatial and temporal variations. Major findings include: (a) different phylogenetic diversity of isolates, dominated by Actinobacteria and fungi, compared to the culture-independent diversity, in which Proteobacteria and Firmicutes were more frequent; (b) cultivation of a novel alphaproteobacterium; (c) dominance of Cyanobacteria among the SSU rRNA gene clones from the 1729.75-m ice; (d) identification of Archaea by NGS that are rarely detected in glacial ice; (e) detection of one or two dominant but different genera among the NGS sequences from each sample; (f) finding dominance of Planococcaceae over Bacillaceae among Firmicutes in the brittle and the 2051.5-m ice. The overall beta diversity between the studied ice core samples examined at the phylum/class level for each approach showed that the population structure of the brittle ice was significantly different from the two deep clathrated ice samples and the shallow ice core