Human Embryonic Stem Cell Technology: Large Scale Cell Amplification and Differentiation

Embryonic stem cells (ESC) hold the promise of overcoming many diseases as potential sources of, for example, dopaminergic neural cells for Parkinson’s Disease to pancreatic islets to relieve diabetic patients of their daily insulin injections. While an embryo has the innate capacity to develop fully functional differentiated tissues; biologists are finding that it is much more complex to derive singular, pure populations of primary cells from the highly versatile ESC from this embryonic parent. Thus, a substantial investment in developing the technologies to expand and differentiate these cells is required in the next decade to move this promise into reality. In this review we document the current standard assays for characterising human ESC (hESC), the status of ‘defined’ feeder-free culture conditions for undifferentiated hESC growth, examine the quality controls that will be required to be established for monitoring their growth, review current methods for expansion and differentiation, and speculate on the possible routes of scaling up the differentiation of hESC to therapeutic quantities

A fast assay for developing serum free media for cord blood haematopoietic cells

10.1007/BF00152447Biotechnology Techniques98577-582BTEC

Hyper-stimulation of monoclonal antibody production by high osmolarity stress in eRDF medium

Journal of Biotechnology373265-275JBIT

Design, simulation, and optimization of a large-scale monoclonal antibody production plant: Upstream design

Pharmaceutical Engineering24642-60PHEN

Enhanced IgG production in eRDF media with and without serum. A comparative study

Journal of Immunological Methods1671-2109-119JIMM

Defined and serum-free media support undifferentiated human embryonic stem cell growth

10.1089/scd.2009.0210Stem Cells and Development196753-761SCDT

Activated T cells modulate immunosuppression by embryonic-and bone marrow-derived mesenchymal stromal cells through a feedback mechanism

10.3109/14653249.2011.635853Cytotherapy143274-284CYTR

Influencing the Fate of Cardiac and Neural Stem Cell Differentiation Using Small Molecule Inhibitors of ALK5

10.1002/sctm.17-0246Stem Cells Translational Medicine710709-72

Knockdown of Oct-4 or Sox-2 attenuates neurogenesis of mouse embryonic stem cells

10.1089/scd.2006.0099Stem Cells and Development163413-420SCDT