Effects of waterborne ZnO nanoparticles and Zn2+ions on the gills of rainbow trout (Oncorhynchus mykiss): Bioaccumulation, histopathological and ultrastructural changes

The aim of this study was comparing the toxic effects of zinc oxide nanoparticles (ZnO NPs) versus zinc ions (Zn2+) at a high non-lethal (500μg/L) and a low environmental relevant (0.05μg/L) concentrations on gills of rainbow trout (Oncorhynchus mykiss) following 14 days of waterborne exposure. Structural alterations, histopathological anomalies, and zinc bioaccumulation were investigated in the gills using field emission scanning electron microscopy (FESEM), hematoxylin and eosin staining (H&E), and graphite furnace atomic absorption spectrophotometry (GFAAS) respectively. Some damages such as shortening and fusion of secondary lamellae, surface epithelium hypertrophy, and hyperplasia of the primary lamellae were observed in the gill tissue. Histopathological alterations of gills were minimum in both 659777250 exposed (control) fish and fish exposed to 0.05μg/L Zn2+. The severity of gill damages were higher in fish exposed to 500μg/L ZnO NPs compared to 500μg/L Zn2+and 0.05μg/L ZnO NPs. The Zn accumulation in the gills was concentration-dependent such that bioaccumulation order was as 500μg/L Zn2+> 500μg/L ZnO NPs � 0.05μg/L Zn2+> 0.05μg/L ZnO NPs> control. In summary, the results of present study showed that although the accumulation capability of Zn2+was higher than ZnO NPs, but NPs cause more structural damages to gills compare to ions. © 2018, Central Fisheries Research Inst. All rights reserved

Effects of waterborne ZnO nanoparticles and Zn2+ions on the gills of rainbow trout (Oncorhynchus mykiss): Bioaccumulation, histopathological and ultrastructural changes

The aim of this study was comparing the toxic effects of zinc oxide nanoparticles (ZnO NPs) versus zinc ions (Zn2+) at a high non-lethal (500μg/L) and a low environmental relevant (0.05μg/L) concentrations on gills of rainbow trout (Oncorhynchus mykiss) following 14 days of waterborne exposure. Structural alterations, histopathological anomalies, and zinc bioaccumulation were investigated in the gills using field emission scanning electron microscopy (FESEM), hematoxylin and eosin staining (H&E), and graphite furnace atomic absorption spectrophotometry (GFAAS) respectively. Some damages such as shortening and fusion of secondary lamellae, surface epithelium hypertrophy, and hyperplasia of the primary lamellae were observed in the gill tissue. Histopathological alterations of gills were minimum in both 659777250 exposed (control) fish and fish exposed to 0.05μg/L Zn2+. The severity of gill damages were higher in fish exposed to 500μg/L ZnO NPs compared to 500μg/L Zn2+and 0.05μg/L ZnO NPs. The Zn accumulation in the gills was concentration-dependent such that bioaccumulation order was as 500μg/L Zn2+> 500μg/L ZnO NPs � 0.05μg/L Zn2+> 0.05μg/L ZnO NPs> control. In summary, the results of present study showed that although the accumulation capability of Zn2+was higher than ZnO NPs, but NPs cause more structural damages to gills compare to ions. © 2018, Central Fisheries Research Inst. All rights reserved

Do we know the mass of a black hole? Mass of some cosmological black hole models

Using a cosmological black hole model proposed recently, we have calculated the quasi-local mass of a collapsing structure within a cosmological setting due to different definitions put forward in the last decades to see how similar or different they are. It has been shown that the mass within the horizon follows the familiar Brown-York behavior. It increases, however, outside the horizon again after a short decrease, in contrast to the Schwarzschild case. Further away, near the void, outside the collapsed region, and where the density reaches the background minimum, all the mass definitions roughly coincide. They differ, however, substantially far from it. Generically, we are faced with three different Brown-York mass maxima: near the horizon, around the void between the overdensity region and the background, and another at cosmological distances corresponding to the cosmological horizon. While the latter two maxima are always present, the horizon mass maxima is absent before the onset of the central singularity.Comment: 11 pages, 8 figures, revised version, accepted in General Relativity and Gravitatio

Histopathological alterations in the gill of zebrafish (Danio rerio) exposed to Cr and Ba doped TiO2 nanoparticles

In order to improve the photocatalytic efficiency of titanium dioxide, doping it with metals has been suggested; however, toxicity studies of metal-doped nanoparticles on aquatic organisms are not completely known. Therefore, the purpose of this study was to evaluate the short term effect of exposure to Cr and Ba doped TiO2 nanoparticles (NPs) using gill histopathology of zebrafish (Danio rerio) under laboratory condition. In this study, four concentration levels, 1, 10, 32, and 100 mg L-1 of each Cr and Ba doped TiO2 NPs as well as a control group were used. After 4 days of exposure, from each experimental group, three samples of fish gill prepared to study the classic histology (haematoxylin - eosin). The results of this study showed that the Cr and Ba doped TiO2 NPs can cause gill alterations including aneurism, dilated and clubbed tips, hyperplasia, oedema, curvature, fusion of lamellae, increase of mucous secretion, and proliferation in the erythrocytes of cartilaginous core. The extent of tissue damages increased by concentration levels of NPs; however, the severity of injouries caused by Cr was more notable than that of observed by Ba. © 2016 BIOFLUX SRL. All rights reserved

The effect of sub-epineural platelet-rich plasma (PRP) on regeneration of the sciatic nerve in a rat model

Background: Peripheral nerve injury is one of the most challenging of modern surgical problem. Recent advances in understanding the physiological and molecular pathways demonstrated the important role of growth factors in peripheral nerve regeneration. Platelet-rich plasma (PRP) is a biological product that has many growth factors. The aim of this study was to investigate the effect of PRP in the regeneration of sciatic nerve crush in the rat model. Methods: In this experimental study that established in the animal lab of the Hazrat Fatemeh Hospital in Tehran from September to October 2013, Twenty-four healthy male Sprague-Dawley rats (200-250 g) were randomly divided into two groups. In all rats the sciatic nerve was cut and then carefully repaired by the tension free method under a light microscope. In group 1, after the repair, 0.05 μL of PRP was injected below the epineurium to the proximal and distal parts of the repaired area. In group 2 the same amount of normal saline was injected to the proximal and distal of the repaired area. After six weeks footprint analysis, neurophysiologic and histopathology evaluations were performed. Results: Significant differences existed between the two groups footprint analysis (P= 0.001). Also the nerve conduction latency test was significantly shorter in PRP group. (1.0233 ms in PRP group and 1.7375 ms in control) (P< 0.001). The average amplitude in the first group and the second group was 7.6250 mv (control) 6.3667 mv that does not show a statistically significant difference (P= 0.093). Significant differences between the two groups in the number of axons of the proximal portion of the study was not seen (P= 0.29). The parameters included number of axons of the proximal and the distal part of axons, the diameter of the distal and proximal axons in the two groups were compared. In the two groups there was statistically significant difference between the above parameters. (P= 0.298). Conclusion: It seems that PRP may have an important role in peripheral nerve regeneration and functional recovery after nerve laceration and repair. Further clinical evaluation recommended. © 2016, Tehran University of Medical Sciences. All rights reserved

Environmental assessment of the Digche region in order to the development of warm-water fish culture

Proposed land area within the Environmental impact assessment study boundary is designated for development of warm-water fish culture and located at "49 '55 ° 54 to" 14 '56 ° 54 east longitude, and "7 '15 37 to" 48 '19 37 north latitude, in the Gonbad Kavous county of Golestan province. Currently in Digcheh there are 47 earthen ponds with different dimensions comprising some 380 ha project's overal footprint with an estimated 324ha net area of production ponds, which involves common carp and chinese carp fish farming. Fresh water supply is provided from Gorgan-Roud to the culture ponds by pumping via some mobile pump through a common earthen channel which is shared with agriculture lands of the rural community. Environmental impact assessment studies for the project which in terms of design features, subject to article 2, paragraph (e) of Environmental impact assessment regulation is necessary in order to resolve problems related to water supply , optimization of fish farm's hydraulic systems through the construction of concrete main intake channel and optimization and renewal of pond's weir and outlet structure, intake and drainage channels of production ponds, and also renovation of water pumping systems wich will be resulting in increased farmed fish production and decreased production costs. For optimization of EIA study and achievements to update information by using Geographic Information System(GIS) software, the maps of the study area were provided with scrutiny. Thus, in first step digital map with a scale of 1:500 to1:2000 in AUTO CAD format that shows the project location, prepared with land surveying. Layers of required maps based on Iran Army Geographical Organization base maps for site with conceptual and spatial information of location were provided. General location map of the region, residential areas and villages, roads and other maps needed in studies us ing these layers were produced. Next, the paper output for Cartography maps were prepared using ARC GIS software. The spatial layers with the help of GIS spatial analysis studies by different expert teams of EIA studies (soil and water pollution , social, economic and cultural studies, biological environment, climate and hydrology) , were performed. The output of digital spatial data, spatial analysis and Cartography as various maps, tables or diagrams and graphical analysis were used in various discipline reports. Since implementing the project can be create different and various changes or effects on environment resources (physical, biological, social, economic and cultural) ,thus knowing how these impacts occur will be assist to prevent or reduce their pursuant negative effects the environment impact assessment studies carried out. In order to conduct decision-making process between action and no action alternatives for perform Digcheh Aquaculture project the Leopold matrix and comparison checklist method has been used. The results of the Leopold matrix shows that the action of Project with environmental mitigation measures (+412) is superior to no action Project (-79). Obviously, the modernization and Optimization of Digcheh warm-water fish farming development project have some adverse effects on the environment like other development projects but it seems with considering mitigation measures and performing Environmental Management Plans with regard to positive outcomes for local area, implementation of this project will be provide a lot of positive or favorable outcome for indigenous peoples and fish farmers. As well as with implemention of the Digcheh project problems such as the loss of water in earthen channels, the overall status of fish farming practices, migration from rural to urban areas , lack of employment and consequently low level of income and welfare of the people will be improved and acquire positive trend in the region Consequently in the EIA study final findings, the implementation of Dighcheh fish culture development project is recommended and emphasized

Susceptibility to mycobacterial disease due to mutations in IL-12R beta 1 in three Iranian patients

Immunogenetics and cellular immunology of bacterial infectious disease

Economical study of the grey mullet (Mugil cephalus) culture in the Golestan province

Striped grey mullet (Mugil cephalus) is a marine fish with high commercial value and widespread distribution. The grey mullet and some other mullets can be found in saltwater, brackish and fershwater habitats at tropical, subtropical and also temporal climates.This species is suitable for aquaculture worldwide due to living in wide ranges of water temperatures and salinities, acceptable growth rate, suitable food conversion ratio, marketability, suitable species for polyculture with shrimps, marine fish and even with freshwater carps. The grey mullet is farming in different countries such as Italy, Northern Africa countries ( Egypt), occupied Palestine, India, Pakistan, Japan, Hong Kong, Taiwan, Vietnam, Indonesia, coastal countries of southern pacific ocean and Hawaii. Ten thousands of fries of this species (average weight and length of 280 mg and 28.3 mm respectively) imported in March 2008 from Egypt. The wild fries colleted from surrounding natural waters of Alexandria. After finishing the quarantine steps, they stocked in four earthern ponds (each with 2500 m2) located at the Gomishan shrimp educational centre at late march in 2008. At the first year of culture the densities were 5000 and 10000 individuals per hectar and each treatment with two replicates. At second years densities changed with 2000 and 2500 ind./hec. and each of traetments divided fishes with average primary weights of 80 and 115 grames. The fries and fish feeding begins in April when the water temperature rise above 18 °C and it cut in fall when the temperature goes under 20°C. They fed twice a day at 8 AM and 2 PM and the amount was 5-7 percents of the fish existent biomass in each ponds. They fed with dry formulated feed made of Mahdaneh Karadj Company (Danso dry feed) both the first and second years of farming. The feed prepared for carps feeding and containded 23±2% crude protein, 10±2% crude lipid, about 7% crude fiber, 15 % ash, 40±2% carbohydrates and with energy content of 4000±200 kcal/kg. After seven months culture period the average weight and length of fish in treatment 5000 ind/hec. were 113.7gr and 21.1cm respectively and in treatment 10000 ind./hec. the average weight and length were 86.6 gr and 19.6 cm respectively. At first year the average survival rate in different treatments was 77 percent. The average FCR in treatments 5000 and 10000 ind./hec. were 3.4 and 3.9 respectively. At second years after six months culture the average weights in pond no. 9 (with primary weight 115 gr and density 2000 ind./hec), pond no. 10 (with primary weight 80 gr and density 2000 ind./hec), pond no. 11 (with primary weight 115 gr and density 2500 ind./hec) and pond no. 12 (with primary weight 80 gr and density 2500 ind./hec) calculated as 476.6, 338.1, 366.5 and 440 gr respectively. The average survival rate and FCR were 93 % and 3.1 respectively. Ponds no. 9 and 11 (fish stocked with primary weight 115 gr in densities both 2000 and 2500 ind./hec) selected as the best economic efficient treatments

Study of penculture of the common carp (Cyprinus carpio L.) fingerlings at the Gorgan Bay to the maturity stage (broodstock management)

The aim of present project is study on the possibilities of brood stock production from fingerlings in the penculture condition and the effect of density on it .this project was done during three years with two different treatment and three repetitions in the Gorgan Bay. First treatment with two fingerlings per square meter and the 2nd with four fingerlings per square meter in each pen.To carry out the project at first fingerlings were brought from breeding and recruitment centre –Sijeval (Kolmeh) and the stocked at the earthen pound with concentrated food .After that they were adaptated with brakish water between 7-10 days and transferred to Gorgan Bay for cultivation with average weight of 93.78±32.66 grams and 20.53±2.37 cm in length. It should be noted that 6 pens (which surrounded by nets in the water) were fixed using mast with 15-20 cm in diameter and 5.5 meter in length , Net mesh size was 16 mm with string number 33 of kapron.Feeding was done according to the program two times by giving concentrated food at the period of cultivation Physic -o -chemical factors were measured. biometery was done monthly at each pen and other fishes (unwanted) were observed at the same time. results indicate that in the 1st and 2nd treatments average survival rate was 52.3 and 41 percent respectively with average weight of 410±64.98 grams and 390±52.8 and 28.3±1.79 cm and 27.66±1.57in length. SPSS used to analysethe data such as average length and weight of fish in the treatments and in different months with ANOVA method (p≤ 0.05) . results show that there is no significant difference between treatments. Autopsy observation also showed that fishes (female and male) are mature and histological studies showed that most of observed eggs are entirely mature with 800-1200 micron diameter gonad ( male) were mature at the pen in the Gorgan Bay condition .The male and female hormones were evaluated. 17-B- Estradiol and 11-keto testosterone hormones for adult fish (female and male) parents respectively are similar in dense with those adult fish before injection to use at the artificial breeding. Maturation were verified by autopsy, histology and hormone studies

Autoantibodies against type I IFNs in patients with life-threatening COVID-19

Interindividual clinical variability in the course of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) infection is vast. We report that at least 101 of 987 patients with life-threatening coronavirus disease 2019 (COVID-19) pneumonia had neutralizing immunoglobulin G (IgG) autoantibodies (auto-Abs) against interferon-w (IFN-w) (13 patients), against the 13 types of IFN-a (36), or against both (52) at the onset of critical disease; a few also had auto-Abs against the other three type I IFNs. The auto-Abs neutralize the ability of the corresponding type I IFNs to block SARS-CoV-2 infection in vitro. These auto-Abs were not found in 663 individuals with asymptomatic or mild SARS-CoV-2 infection and were present in only 4 of 1227 healthy individuals. Patients with auto-Abs were aged 25 to 87 years and 95 of the 101 were men. A B cell autoimmune phenocopy of inborn errors of type I IFN immunity accounts for life-threatening COVID-19 pneumonia in at least 2.6% of women and 12.5% of men