10 research outputs found
Expression of ?-glutamyl transpeptidase in IAR 2 cells cultured on adhesive and nonadhesive substrates
On the possible effect of a magnetic field on the breaking of mechanically loaded covalent chemical bonds
Reduction in egg hatch ofArgas (Argas) reflexus (Fab.) (Acari, Argasidae) due to colchicine
Hepatitis a virus: Structure-functional features of genome, molecular diagnostics, and cultivation
Nucleic Acids-Based Therapeutics in the Battle Against Pathogenic Viruses
For almost three decades, researchers have studied the possibility to use nucleic acids as antiviral therapeutics. In theory, compounds such as antisense oligonucleotides, ribozymes, DNAzymes, and aptamers can be designed to trigger the sequence-specific inhibition of particular mRNA transcripts, including viral genomes. However, difficulties with their efficiency, off-target effects, toxicity, delivery, and stability halted the development of nucleic acid-based therapeutics that can be used in the clinic. So far, only a single antisense drug, Vitravene for the treatment of CMV-induced retinitis in AIDS patients, has made it to the clinic. Since the discovery of RNA interference (RNAi), there is a renewed interest in the development of nucleic acid-based therapeutics. Antiviral RNAi approaches are highly effective in vitro and in animal models and are currently being tested in clinical trials. Here we give an overview of antiviral nucleic acid-based therapeutics. We focus on antisense and RNAi-based compounds that have been shown to be effective in animal model system