Monitoring of yeast metabolism with calorimetry

A system for calorimetric measurements is established in a 1500 L pilot bioreactor. Saccharomyces cerevisiae, baker’s yeast has three metabolic pathways: 1) purely oxidative, glucose consuming; 2) purely reductive, glucose consuming and ethanol producing and 3) purely oxidative, ethanol consuming. Designing experiments to lead the yeast to a single pathway, the accuracy of the calorimetric measurements are verified. Using on-line data of microbial heat production and substrate consumption, the combustion enthalpies of glucose and ethanol in purely oxidative (aerobic) fed batch fermentations are determined as 15900 and 29000 kJ/kg respectively, Combustion enthalpy of glucose in purely reductive (anaerobic) environment is determined as 511 kJ/kg. These values are in good corresponding with literature data. It is now possible to determine the fraction of substrate uptake utilized for energy (catabolism, k) and biosynthesis (anabolism, a) metabolisms on-line. In oxidative (aerobic) fermentations we determined kSox=0.45 for glucose, kEox=0.63 for ethanol and in reductive (anaerobic) fermentations kSred=0.96 for glucose; the anabolism factors are aSox=0.55, aEox=0.37 and aSred=0.04 respectively. The single pathways can occur together so that an experiment is designed with changing environmental conditions to prove the overall calorimetric model. The result is that measured and the calculated microbial heat energy rates are in good accordance. Calorimetric measurements can be used to monitor yeast metabolism on-line, for advanced control strategies or, to predict fermentations or for designing heat exchanger or bioreactor systems

Evaluation by Respiration Measurements (OTR, CTR and RQ) of the Biological Activity in Sludge DigestorsOperatedUnder Microaerobic Conditions

Microbial activity measurements (oxygen transfer rate, OTR, carbon dioxide transfer rate CTR and respiratory quotient, RQ) are used to monitor and control biological processes. In this study, the digestion of activated sludge in microaerobic conditions was evaluated. The changes of biological activity during digestion were monitored by respiration activity. The sludge mixtures used were primary plus activated sludge, and baker’s yeast plus activated sludge. The biological activity was triggered during the experiments by feeding sugar to the mixtures. The results show that increased activity by adding sugar to digested sludge can be monitored by OTR and CTR, whereas RQ can not beused as an indicator

Marktdaten zur Biotechnologie: Produkte und Reaktoren

Copy held by FIZ Karlsruhe; available from UB/TIB Hannover / FIZ - Fachinformationszzentrum Karlsruhe / TIB - Technische InformationsbibliothekSIGLEDEGerman

Bioreaktoren: Ein Leitfaden fuer Anwender

SIGLEAvailable from Gesellschaft fuer Biotechnologische Forschung m.b.H., Braunschweig (DE) / FIZ - Fachinformationszzentrum Karlsruhe / TIB - Technische InformationsbibliothekDEGerman