Gelechiidae Moths Are Capable of Chemically Dissolving the Pollen of Their Host Plants

Background: Many insects feed on pollen surface lipids and contents accessible through the germination pores. Pollen walls, however, are not broken down because they consist of sporopollenin and are highly resistant to physical and enzymatic damage. Here we report that certain Microlepidoptera chemically dissolve pollen grains with exudates from their mouthparts. Methodology/Principal Findings: Field observations and experiments in tropical China revealed that two species of Deltophora (Gelechioidea) are the exclusive pollinators of two species of Phyllanthus (Phyllanthaceae) on which their larvae develop and from which the adults take pollen and nectar. DNA sequences placed the moths and plants phylogenetically and confirmed that larvae were those of the pollinating moths; molecular clock dating suggests that the moth clade is younger than the plant clade. Captive moths with pollen on their mouthparts after 2-3 days of starvation no longer carried intact grains, and SEM photographs showed exine fragments on their proboscises. GC-MS revealed cis-b-ocimene as the dominant volatile in leaves and flowers, but GC-MS analyses of proboscis extracts failed to reveal an obvious sporopollenindissolving compound. A candidate is ethanolamine, which occurs in insect hemolymphs and is used to dissolve sporopollenin by palynologists. Conclusions/Significance: This is the first report of any insect and indeed any animal chemically dissolving pollen

X-ray Flares from Markarian 501

Motivated by the recent finding of hierarchical X-ray flaring phenomenon in Mrk 421, we conducted a systematic search for X-ray flares from Mrk 501, another well-known TeV blazar, by making use of the rich {\em RXTE} archival database. We detected flares over a wide range of timescales, from months down to minutes, as in the case of Mrk 421. However, the flares do not seem to occur nearly as frequently in Mrk 501 as in Mrk 421 on any of the timescales. The flaring hierarchy also seems apparent in Mrk 501, suggesting that it might be common among TeV blazars. The results seem to imply a scale-invariant physical origin of the flares (large or small). The X-ray spectrum of the source shows a general trend of hardening toward the peak of long-duration flares, with indication of spectral hysteresis, which is often seen in TeV blazars. However, the data are not of sufficient quality to allow us to draw definitive conclusions about spectral variability associated with more rapid but weaker flares. We critically examine a reported sub-hour X-ray flare from Mrk 501, in light of intense background flaring activity at the time of the observation, and concluded that the flare is likely an artifact. On the other hand, we did identify a rapid X-ray flare that appears to be real. It lasted only for about 15 minutes, during which the flux of the source varied by about 30%. Sub-structures are apparent in its profile, implying variabilities on even shorter timescales. Such rapid variabilities of Mrk 501 place severe constraints on the physical properties of the flaring region in the jet, which have serious implications on the emission models proposed for TeV blazars.Comment: 23 pages, 11 figures, accepted for publication in Ap

Modeling the Broadband Spectral Energy Distribution of the Microquasars XTE J1550-564 and H 1743-322

We report results from a systematic study of the spectral energy distribution (SED) and spectral evolution of XTE J1550--564 and H 1743--322 in outburst. The jets of both sources have been directly imaged at both radio and X-ray frequencies, which makes it possible to constrain the spectrum of the radiating electrons in the jets. We modelled the observed SEDs of the jet `blobs' with synchrotron emission alone and with synchrotron emission plus inverse Compton scattering. The results favor a pure synchrotron origin of the observed jet emission. Moreover, we found evidence that the shape of the electron spectral distribution is similar for all jet `blobs' seen. Assuming that this is the case for the jet as a whole, we then applied the synchrotron model to the radio spectrum of the total emission and extrapolated the results to higher frequencies. In spite of significant degeneracy in the fits, it seems clear that, while the synchrotron radiation from the jets can account for nearly 100% of the measured radio fluxes, it contributes little to the observed X-ray emission, when the source is relatively bright. In this case, the X-ray emission is most likely dominated by emission from the accretion flows. When the source becomes fainter, however, the jet emission becomes more important, even dominant, at X-ray energies. We also examined the spectral properties of the sources during outbursts and the correlation between the observed radio and X-ray variabilities. The implication of the results is discussed.Comment: 9 pages, 11 figures, MNRAS, accepted; the paper has been much expanded (e.g., arguments strengthened, another source H 1743-322 added) and rewritten (e.g., title changed, abstract revised); the main conclusions remain unchange

X-ray Spectral Variability of TeV Blazars during Rapid Flares

The spectral energy distribution (SED) of TeV blazars peaks both at keV and TeV energies. The X-ray emission is generally believed to originate in the synchrotron emission from relativistic electrons (and positrons) in the jet of these sources, while the origin of the gamma-ray emission is still being debated. We report results from a systematic study of X-ray spectral variability of Mrk 421 and Mrk 501 during individual flares that last for several days, making use of some of the high-quality data that have recently become available. The X-ray spectra of the two sources fall on the opposite sides of the synchrotron peak of their respective SEDs, so they together may offer additional insights into the physical origin of X-ray variability. We modeled each of the time-resolved X-ray spectra over a {\em single} flare by adopting a homogeneous spatial distribution and an instantaneous power-law spectral distribution for the emitting particles. We focused on the variation of four key parameters: particle spectral index, maximum Lorentz factor, energy density, and magnetic field. While there is considerable degeneracy in the fits, we show that, in order to account for the X-ray spectral variability observed in Mrk 421, at least three of the parameters are required to vary in most cases, with the spectral index being one of them. The observations of Mrk 501 support the conclusion, although the quality of the data is not as good. We discuss the implications of the results.Comment: 17 pages, 4 figures, 4 tables, accepted by ApJ; one typo (unit of E_tot) correcte

Src activates Abl to augment Robo1 expression in order to promote tumor cell migration

Cell migration is an essential step in cancer invasion and metastasis. A number of orchestrated cellular events involving tyrosine kinases and signaling receptors enable cancer cells to dislodge from primary tumors and colonize elsewhere in the body. For example, activation of the Src and Abl kinases can mediate events that promote tumor cell migration. Also, activation of the Robo1 receptor can induce tumor cell migration. However, while the importance of Src, Abl, and Robo1 in cell migration have been demonstrated, molecular mechanisms by which they collectively influence cell migration have not been clearly elucidated. In addition, little is known about mechanisms that control Robo1 expression. We report here that Src activates Abl to stabilize Robo1 in order to promote cell migration. Inhibition of Abl kinase activity by siRNA or kinase blockers decreased Robo1 protein levels and suppressed the migration of transformed cells. We also provide evidence that Robo1 utilizes Cdc42 and Rac1 GTPases to induce cell migration. In addition, inhibition of Robo1 signaling can suppress transformed cell migration in the face of robust Src and Abl kinase activity. Therefore, inhibitors of Src, Abl, Robo1 and small GTPases may target a coordinated pathway required for tumor cell migration

Rotational velocities of low-mass stars in the Pleiades and Hyades

We have obtained high-resolution spectra of 89 M dwarf members of the Pleiades and Hyades and have derived radial velocities, H-alpha equivalent widths, and spectroscopic rotational velocities for these stars. Typical masses of the newly-observed Pleiades and Hyades stars are ~ 0.4 M_{\sun} and ~ 0.2 M_{\sun}, respectively. We combine our new observations with previously published data to explore the rotational evolution of young stars with M < 0.4 M_\sun. The average rotation rate in the Hyades (age 600 Myr) is about 0.4 that of the Pleiades (110 Myr), and the mean equivalent widths of H-alpha are also lower. As found in previous studies, the correlation between rotation and chromospheric activity is identical in both clusters, implying that the lower activity in the Hyades is a result of the lower rotation rates. We show that a simple scaling of the Pleiades rotational distribution for M \leq 0.4 M_{\sun}, corrected for the effects of structural evolution, matches that of the Hyades if the average angular momentum loss from the Pleiades to the Hyades age is factor of \approx 6. This suggests that the distribution of initial angular momenta and disk-locking lifetimes for the lowest mass stars was similar in both clusters. We argue that this result provides further evidence for a saturation of the angular momentum loss rate at high rotational velocities.Comment: 22 pages, 11 figures, accepted for publication in The Astronomical Journal, tentatively scheduled for March 200

The association between protease inhibitors and anal cancer outcomes in veterans living with HIV treated with definitive chemoradiation: a retrospective study

Background: The incidence of anal squamous cell carcinoma has been increasing, particularly in people living with HIV (PLWH). There is concern that radiosensitizing drugs, such as protease inhibitors, commonly used in the management of HIV, may increase toxicities in patients undergoing chemoradiation. This study examines treatment outcomes and toxicities in PLWH managed with and without protease inhibitors who are receiving chemoradiation for anal cancer. Methods: Patient demographic, HIV management, and cancer treatment information were extracted from multiple Veterans Affairs databases. Patients were also manually chart reviewed. Among PLWH undergoing chemoradiation for anal carcinoma, therapy outcomes and toxicities were compared between those treated with and without protease inhibitors at time of cancer treatment. Statistical analysis was performed using chi-square, Cox regression analysis, and logistic regression. Results: A total of 219 PLWH taking anti-retroviral therapy undergoing chemoradiation for anal cancer were identified and included in the final analysis. The use of protease inhibitors was not associated with any survival outcome including colostomy-free survival, progression-free survival, or overall survival (all adjusted hazard ratio p-values\u3e 0.05). Regarding toxicity, protease inhibitor use was not associated with an increased odds of hospitalizations or non-hematologic toxicities; however, protease inhibitor use was associated with increased hospitalizations for hematologic toxicities, including febrile neutropenia (p \u3c 0.01). Conclusion: The use of protease inhibitors during chemoradiation for anal carcinoma was not associated with any clinical outcome or increase in non-hematologic toxicity. Their use was associated with increased hospitalizations for hematologic toxicities. Further prospective research is needed to evaluate the safety and efficacy of protease inhibitors for patients undergoing chemoradiation

Improved Siderotic Nodule Detection in Cirrhosis with Susceptibility-Weighted Magnetic Resonance Imaging: A Prospective Study

BACKGROUND: Hepatic cirrhosis is a common pathway of progressive liver destruction from multiple causes. Iron uptake can occur within the hepatic parenchyma or within the various nodules that form in a cirrhotic liver, termed siderotic nodules. Siderotic nodule formation has been shown to correlate with inflammatory activity, and while the relationship between siderotic nodule formation and malignancy remains unclear, iron distribution within hepatic nodules has known implications for the detection of hepatocellular carcinoma. We aimed to evaluate the role of abdominal susceptibility-weighted imaging in the detection of siderotic nodules in cirrhotic patients. METHODOLOGY/PRINCIPAL FINDINGS: Forty-six (46) cirrhotic patients with at least one siderotic nodule detected on previous imaging underwent both computed tomography and magnetic resonance imaging (T1-, T2-, T2*-, and susceptibility-weighted imaging) at 3.0 Tesla. Imaging data was independently analyzed by two radiologists. Siderotic nodule count was determined for each modality and imaging sequence. For each magnetic resonance imaging technique, siderotic nodule conspicuity was assessed on a 3 point scale (1 = weak, 2 = moderate, 3 = strong). More nodules were detected by susceptibility weighted imaging (n = 2935) than any other technique, and significantly more than by T2* weighted imaging (n = 1696, p<0.0001). Lesion conspicuity was also highest with susceptibility-weighted imaging, with all nodules found to be moderate (n = 6) or strong (n = 40); a statistically significant difference (p<0.001). CONCLUSIONS: Susceptibility-weighted imaging had the greatest lesion conspicuity and detected the highest number of siderotic nodules suggesting it is the most sensitive imaging technique to detect siderotic nodules in cirrhotic patients

A Mutation in Intracellular Loop 4 Affects the Drug-Efflux Activity of the Yeast Multidrug Resistance ABC Transporter Pdr5p

Multidrug resistance protein Pdr5p is a yeast ATP-binding cassette (ABC) transporter in the plasma membrane. It confers multidrug resistance by active efflux of intracellular drugs. However, the highly polymorphic Pdr5p from clinical strain YJM789 loses its ability to expel azole and cyclohexmide. To investigate the role of amino acid changes in this functional change, PDR5 chimeras were constructed by segmental replacement of homologous BY4741 PDR5 fragments. Functions of PDR5 chimeras were evaluated by fluconazole and cycloheximide resistance assays. Their expression, ATPase activity, and efflux efficiency for other substrates were also analyzed. Using multiple lines of evidence, we show that an alanine-to-methionine mutation at position 1352 located in the predicted short intracellular loop 4 significantly contributes to the observed transport deficiency. The degree of impairment is likely correlated to the size of the mutant residue