Dietary control of the renal reabsorption and excretion of α2u-globulin

Dietary protein supply is a factor in controlling the excretion of proteins in the urine. As early as 1926, Addis, Mackay, and Mackay observed that male rats on a 69% protein diet excreted more urinary proteins than did those on a 17% diet [1]. Protein deficiency had the opposite effect, resulting in a suppression of the proteinuria [2]. Of the total urinary proteins excreted by the adult male rat, approximately 30% is a sex-dependent globulin called α2u [3,4], which is synthesized by the liver [5] and controlled synergistically by androgens and glucocorticoids [6]. Dietary protein supply also had a profound influence on the excretion of α2u [4]. On a 0% casein diet, the excretion was reduced to approximately 1 mg/24 hours compared with a normal of 10 to 15mg. On a 50% casein diet, rats excreted 30 to 50 mg/24 hours, an increase of more than 100% above the normal [4].Early studies also suggested that high protein diets exaggerated the leakage of plasma proteins caused by a spontaneous nephrotic syndrome observed in male rats [7, 8]. Rats previously castrated did not exhibit an increased excretion of urinary protein on a 50% casein diet, whereas supplementation with testosterone restored the augmented proteinuria [9]. This suggested that the elevated excretion of urinary protein was dependent on the presence of androgens. It is now known that a high-protein diet caused an increased excretion of α2u without at the same time leading to a compensatory, stimulated hepatic biosynthesis. Conceivably, the increased excretion of α2u was the consequence of an altered state of renal reabsorption [4]. The purpose of the present communication was to compare the degree of renal reabsorption under three different dietary conditions and to determine whether the kidneys controlled the urinary excretion of α2u by altering its reabsorption

Recombination dramatically speeds up evolution of finite populations

We study the role of recombination, as practiced by genetically-competent bacteria, in speeding up Darwinian evolution. This is done by adding a new process to a previously-studied Markov model of evolution on a smooth fitness landscape; this new process allows alleles to be exchanged with those in the surrounding medium. Our results, both numerical and analytic, indicate that for a wide range of intermediate population sizes, recombination dramatically speeds up the evolutionary advance

Physics of Quantum Relativity through a Linear Realization

The idea of quantum relativity as a generalized, or rather deformed, version of Einstein (special) relativity has been taking shape in recent years. Following the perspective of deformations, while staying within the framework of Lie algebra, we implement explicitly a simple linear realization of the relativity symmetry, and explore systematically the resulting physical interpretations. Some suggestions we make may sound radical, but are arguably natural within the context of our formulation. Our work may provide a new perspective on the subject matter, complementary to the previous approach(es), and may lead to a better understanding of the physics.Comment: 27 pages in Revtex, no figure; proof-edited version to appear in Phys.Rev.

Analysis of molecular diversity, population structure and linkage disequilibrium in a worldwide survey of cultivated barley germplasm (Hordeum vulgare L.)

BACKGROUND: The goal of our study was a systematic survey of the molecular diversity in barley genetic resources. To this end 953 cultivated barley accessions originating from all inhabited continents except Australia were genotyped with 48 SSR markers. Molecular diversity was evaluated with routine statistics (allelic richness, gene diversity, allele frequency, heterozygosity and unique alleles), Principal Coordinate Analysis (PCoA), and analysis of genome-wide linkage disequilibrium. RESULTS: A genotyping database for 953 cultivated barley accessions profiled with 48 SSR markers was established. The PCoA revealed structuring of the barley population with regard to (i) geographical regions and (ii) agronomic traits. Geographic origin contributed most to the observed molecular diversity. Genome-wide linkage disequilibrium (LD) was estimated as squared correlation of allele frequencies (r(2)). The values of LD for barley were comparable to other plant species (conifers, poplar, maize). The pattern of intrachromosomal LD with distances between the genomic loci ranging from 1 to 150 cM revealed that in barley LD extended up to distances as long as 50 cM with r(2 )> 0.05, or up to 10 cM with r(2 )> 0.2. Few loci mapping to different chromosomes showed significant LD with r(2 )> 0.05. The number of loci in significant LD as well as the pattern of LD were clearly dependent on the population structure. The LD in the homogenous group of 207 European 2-rowed spring barleys compared to the highly structured worldwide barley population was increased in the number of loci pairs with r(2 )> 0.05 and had higher values of r(2), although the percentage of intrachromosomal loci pairs in significant LD based on P < 0.001 was 100% in the whole set of varieties, but only 45% in the subgroup of European 2-rowed spring barleys. The value of LD also varied depending on the polymorphism of the loci selected for genotyping. The 17 most polymorphic loci (PIC > 0.80) provided higher LD values as compared to 19 low polymorphic loci (PIC < 0.73) in both structured (all accessions) and non-structured (European 2-rowed spring varieties) barley populations. CONCLUSION: A global population of cultivated barley accessions was highly structured. Clustering highlighted the accessions with the same geographic origin, as well as accessions possessing similar agronomic characters. LD in barley extended up to 50 cM, and was strongly dependent on the population structure. The data on LD were summarized as a genome-wide LD map for barley

Analytical study of the effect of recombination on evolution via DNA shuffling

We investigate a multi-locus evolutionary model which is based on the DNA shuffling protocol widely applied in \textit{in vitro} directed evolution. This model incorporates selection, recombination and point mutations. The simplicity of the model allows us to obtain a full analytical treatment of both its dynamical and equilibrium properties, for the case of an infinite population. We also briefly discuss finite population size corrections

NMFS / Interagency Working Group Evaluation of CITES Criteria and Guidelines.

EXECUTIVE SUMMARY: At present, the Convention on International Trade in Endangered Species of Wild Fauna and Flora (CITES) criteria used to assess whether a population qualifies for inclusion in the CITES Appendices relate to (A) size of the population, (B) area of distribution of the population, and (C) declines in the size of the population. Numeric guidelines are provided as indicators of a small population (less than 5,000 individuals), a small subpopulation (less than 500 individuals), a restricted area of distribution for a population (less than 10,000 km2), a restricted area of distribution for a subpopula-tion (less than 500 km2), a high rate of decline (a decrease of 50% or more in total within 5 years or two generations whichever is longer or, for a small wild population, a decline of 20% or more in total within ten years or three generations whichever is longer), large fluctuations (population size or area of distribution varies widely, rapidly and frequently, with a variation greater than one order of magnitude), and a short-term fluctuation (one of two years or less). The Working Group discussed several broad issues of relevance to the CITES criteria and guidelines. These included the importance of the historical extent of decline versus the recent rate of decline; the utility and validity of incorporating relative population productivity into decline criteria; the utility of absolute numbers for defining small populations or small areas; the appropriateness of generation times as time frames for examining declines; the importance of the magnitude and frequency of fluctuations as factors affecting risk of extinction; and the overall utility of numeric thresh-olds or guidelines

Excitation of guided waves in layered structures with negative refraction

We study the electromagnetic beam reflection from layered structures that include the so-called double-negative materials, also called left-handed metamaterials. We predict that such structures can demonstrate a giant lateral Goos-Hanchen shift of the scattered beam accompanied by splitting of the reflected and transmitted beams due to the resonant excitation of surface waves at the interfaces between the conventional and double-negative materials as well as due to excitation of leaky modes in the layered structures. The beam shift can be either positive or negative, depending on the type of the guided waves excited by the incoming beam. We also perform finite-difference time-domain simulations and confirm the major effects predicted analytically.Comment: 13 pqages, 10 figures. Also available at http://www.opticsexpress.org/abstract.cfm?URI=OPEX-13-2-48

Self-Reported Physical Activity and Myocardial Flow Reserve in Postmenopausal Women at Risk for Cardiovascular Disease

Background: Regular exercise protects against coronary heart disease (CHD) events and improves vascular reactivity. Exercise effects on myocardial flow reserve (MFR) are not well studied. Methods: We performed dynamic N-13 ammonia positron emission tomography (PET) in 16 postmenopausal women (60 ± 6 years) to measure myocardial blood flow (MBF) and MFR. We also obtained information from each woman on her self-reported physical activity. Results: Of the 16 patients, 6 reported moderate regular physical activity, and 10 did not. Women who reported regular, at least moderate physical activity had a higher percentage increase in adenosine MBF from rest compared with women who did not exercise (268% vs. 129%, p = 0.04) and had a significantly higher mean maximal MFR (3.68 vs. 2.29, p = 0.04). Conclusions: These findings provide further mechanistic support for the beneficial cardiovascular effects of exercise.Peer Reviewedhttp://deepblue.lib.umich.edu/bitstream/2027.42/63137/1/jwh.2006.15.45.pd