THE FORMATION OF EFFECTIVE MANAGEMENT OF TECHNICAL-GEOLOGICAL SYSTEMS IN GEOLOGICAL SUBSOIL: REALITY AND PERSPECTIVES

A technical-geological system (TGS) consists of geological environment, man-made objects (techno-sphere) and management system, which are divided into a number of individual components. This all can be considered as natural - anthropogenic complex. The effective functioning of such complex set as TGS is provided by perfect theory, management methodology and by the degree of information fullness and its reliability. We study the “mining deposit-geological environment” systems as technical-geological system (TGS) with the following constituents: geological object rated as deposit facility with defined economic assessment, technical equipment for the conduction of extraction works and management system of all processes in TGS. The investigation of mineral deposits as TGS has three practical aspects: 1) it refers to the study of the geological environment as the environment of human living; 2) it is associated with the study of geological bodies as natural resources; 3) it is associated with retrospective research based on the study of dynamical systems, as well as with determination of the forecast of mineral resources exploration. Mastering of mining deposit should be provided with such information capabilities that match the complexity of the TGS, in order to be able to control its condition, relationships, and flows of matter, energy and information. Accordingly, this situation will provide TGS management the capacity to bring economic development in the fields of deposit development to order. The basis of management is the development of the impact of the managing institution on the object of management, which is revealed sin formation process that provides acquisition, processing and analysis of obtained data to build a model of TGS state, using the estimated parameters, develop­ment of recommendations for the implementation of managing in flounce. This approach can be considered as theoretical basis of rational subsoil use. The effective management of the TGS resource potential has to be a flexible system that will change, adapt to market conditions, thus ensuring maximum utilization of mineral resources while preserving the environment. Based on this, the systematic approach to effectively manage resource potential TGS is proposed. The economic evaluation of reserves and mineral resources should be conducted on each stage of the development of the mineral resources complex for the purposes of increasing the validity of decisions and formulation of a comprehensive development strategy based on the standards of international level of the system CRIRSCO (Committee for Mineral Reserves International Reporting Standards). CRIRSCO implementation is held starting with the assessment of the efficiency of the industry as a whole and should serve as a basis for the selection of priority directions of its development at all stages of geological prospecting and operation of mineral resources

Label-free measuring and mapping of binding kinetics of membrane proteins in single living cells

Membrane proteins (MPs) mediate a variety of cellular responses to extracellular signals. While MPs are intensely studied for their values as disease biomarkers and therapeutic targets, in situ investigation of binding kinetics of MPs with their ligands has been a challenge. Traditional approaches isolate MPs and then study them ex situ, which does not accurately reflect their native structures and functions. We present here a label-free plasmonic microscopy method to map the local binding kinetics of MPs in their native environment. This new analytical method can perform simultaneous plasmonic and fluorescence imaging, thus making it possible to combine the strengths of both label-based and label-free techniques in one system. Using this method, we have determined the distribution of MPs on the surface of single cells, and the local binding kinetic constants of different MPs. Furthermore, we have studied the polarization of the MPs on the cell surface during chemotaxis