Genetic variation of RFXANK gene in Stavropol sheep breed

Using of NimbleGen sequencing technology for detection of polymorphisms RFXANK gene in Stavropolsheep breed, we found 20 SNPs. That is, two SNP in exons - c.63C>A (non synonymous) in exon II and c.498G>A in exon VI (synonymous). Others SNP is in introns: c.-343T>C, c.- 111T>G, c.187+328G>A, c.338-94G>A, c.632–124G>A, c.713-82G>A, c.187+62G>A, c.188-588G>C, c.188- 127C>T, c.565-44A>G, c.712+309C>T, c.712+354C>T, c.712+309C>T, c.187+394C>T, c.187+469A>G, c.187+71G>C, c.188-670A>G, c.713-107G>T. Some of them are presented together

НЕОПЕРАТИВНОЕ РАСПРАВЛЕНИЕ ИНВАГИНАЦИИ КИШЕЧНИКА У ДЕТЕЙ МЕТОДОМ ГИДРОЭХОКОЛОНОСКОПИИ

ABSTRACT. The possibilities of ultrasonography in the diagnosis of intussusception in children and disinvagination by hydroechocolonoscopy have been studied. The study was conducted in 2014–2015 at the Children’s Regional hospital of Tver. The age of children varied from 3 months up to 12 years. The disease duration before admission was 3–63 hours. The X-ray study confirmed the intussusception in all patients. In all cases, mesenteric lymphadenitis was revealed. Of 28 children, disinvagination was sucsessfuly performed with an aid of hydroechocolonoscopy in 26 (92.8%) patients. In 2 cases (7.2%), we failed to eliminate intussusception and children underwent the surgery. Three patients had recurrence; re-elimination of intussusception with hydroechocolonoscopy was performed. No complications were observed after disinvagination. Conclusion: The use of ultrasound in the diagnosis and treatment of intussusception eliminates radiation exposure, and allows the cause of intussusceptions to be determined as well. Disinvagination with hydroechocolonoscopy is technically simple, highly effective and safe. РЕЗЮМЕ. Изучены возможности УЗИ в диагностике инвагинации кишечника (ИК) у детей и дезинвагинации методом гидроэхоколоноскопии (ГЭС). Исследование проведено с 2014 по 2015 г. на базе Детской областной больницы Твери. Возраст детей от 3 мес до 12 лет. Длительность заболевания до поступления в стационар — 3–63 ч. При УЗИ диагноз инвагинации кишечника подтвержден у всех пациентов. Во всех случаях выявлен мезентериальный лимфаденит. Из 28 детей дезинвагинация методом ГЭС успешно выполнена 26 пациентам (92,8%). У 2 (7,2%) инвагинат расправить не удалось — дети были оперированы. У 3 пациентов возник рецидив заболевания, выполнено повторное расправление ИК методом ГЭС. Осложнений после дезинвагинаций не было. Заключение: использование УЗИ в диагностике и лечении инвагинации кишечника у детей полностью исключает лучевую нагрузку, а также позволяет установить причину инвагинации. Дезинвагинация методом ГЭС технически проста, высокоэффективна и безопасна.

DISTRIBUTION AND ECOLOGICAL CHARACTERISTICS OF HYALOMMA IXODID TICKS IN THE ECOSYSTEMS OF THE STAVROPOL REGION

Aim. To determine the characteristics of the modern dissemination, distribution and seasonal activity of Hyalomma ixodid ticks in the Stavropol region.Methods. The study of the spread of Ixodes Hyalomma ticks was conducted in all administrative districts of the Stavropol Territory in the period of 2000-2015. Collection of ixodid ticks in natural habitats, home to wild mammals and birds, was carried out according to conventional techniques.Results. Hyalomma marginatum is a two-host tick. In the region, H. marginatum of an adult stage becomes active in early spring (late March - early April); appearance of the larvae is observed in early July; the nymphs in the third decade of July. The peculiarity of biological development of H. scupense is the activation of adult species in the cold season (winter); development is only of one-host cycle. The peak number of ticks of an adult stage in cattle falls on the last days of January and February.Conclusion. Hyalomma ixodid ticks in the Stavropol region are distributed mosaicly, with the dominance of some species depending on climatic and landscape-geographical features of the territories they inhabit. The dominant species are H. marginatum and H. scupense, but H. anatolicum tick species occur sporadically in the east region

NON-SURGICAL ELIMINATION OF INTUSSUSCEPTION IN CHILDREN WITH HYDROECHOСOLONOSCOPY

ABSTRACT. The possibilities of ultrasonography in the diagnosis of intussusception in children and disinvagination by hydroechocolonoscopy have been studied. The study was conducted in 2014–2015 at the Children’s Regional hospital of Tver. The age of children varied from 3 months up to 12 years. The disease duration before admission was 3–63 hours. The X-ray study confirmed the intussusception in all patients. In all cases, mesenteric lymphadenitis was revealed. Of 28 children, disinvagination was sucsessfuly performed with an aid of hydroechocolonoscopy in 26 (92.8%) patients. In 2 cases (7.2%), we failed to eliminate intussusception and children underwent the surgery. Three patients had recurrence; re-elimination of intussusception with hydroechocolonoscopy was performed. No complications were observed after disinvagination. Conclusion: The use of ultrasound in the diagnosis and treatment of intussusception eliminates radiation exposure, and allows the cause of intussusceptions to be determined as well. Disinvagination with hydroechocolonoscopy is technically simple, highly effective and safe

The use of selenium for protective and stimulating effects on plants when soil is contaminated with cadmium

Abstract At the present time one of the tasks of modern agricultural industry consists in obtaining the ecologically safe and clean products. Contamination of soils with heavy metals due to an anthropogenic impact drives up their content in the composition of plant products. This shapes not only a reduction in crop yields, but also a deterioration in products quality. Within the terms of vegetation research in soil culture, there has been studied the protective and stimulating effect of sodium selenite upon the adaptive capacity of spring wheat plants of the variety Zlata under conditions of oxidative stress due to the soil contamination with cadmium. There has been studied the effect of different methods of sodium selenite application on the yield of spring wheat and the plants photosynthetic activity, depending on the level of soil contamination with cadmium. The object of research was a spring wheat variety Zlata. Plants have been cultivated in a greenhouse trial under soil culture conditions in Mitscherlich-vessels with a capacity of 6 kg of soil. Sod-podzolic soil has been used for research. Sodium selenite was introduced in three ways: pre-sowing seed treatment, foliar treatment of vegetative plants at the beginning of stage VI of organogenesis - the end of the tillering phase - the beginning of the stem-extension phase and the application of a salt solution into the soil when packing the vessels. The control samples represented variants without sodium selenite. To assess the plants photosynthetic productivity, there has been determined the chlorophyll content in plant leaves. The research results made it possible to determine the protective effect of sodium selenite on the adaptive capacity of plants under conditions of oxidative stress due to the soil contamination with cadmium. The increase in the adaptive capacity of plants manifested itself through the decrease in yield diminishing due to the improvement of conditions for fertile florets and ear initiations on the vegetative apex, as well as the development of flowers into grains, which contributed to increase in the grain content of the spike. The stimulating effect of selenium on the intensity of photosynthetic processes has been revealed, which showed not only the increase of chlorophylls content, but also the ratio changes of chlorophylls a and b