A unique olfactory bulb microcircuit driven by neurons expressing the precursor to glucagon-like peptide 1

The presence of large numbers of local interneurons in the olfactory bulb has demonstrated an extensive local signaling process, yet the identification and purpose of olfactory microcircuits is poorly explored. Because the discrimination of odors in a complex environment is highly dependent on the tuning of information by local interneurons, we studied for the first time the role of preproglucagon (PPG) neurons in the granule cell layer of the olfactory bulb. Combining electrophysiological recordings and confocal microscopy, we discovered that the PPG neurons are a population of cells expressing the precursor of glucagon-like peptide 1 and are glutamatergic; able to modulate the firing pattern of the mitral cells (M/TCs). Optogenetic activation of PPG neurons resulted in a mixed excitation and inhibition that created a multiphasic response shaping the M/TCs firing pattern. This suggests that PPG neurons could drive neuromodulation of the olfactory output and change the synaptic map regulating olfactory coding

Associations between pQCT-based fat and muscle area and density and DXA-based total and leg soft tissue mass in healthy women and men

Peripheral Quantitative Computed Tomography (pQCT) can be used for muscle and fat area and density assessments. These may independently influence muscle and fat mass measurements from Dual Energy X-ray Absorptiometry (DXA). Objective: To determine associations between pQCT-derived soft tissue density and area measures and DXA-derived soft tissue mass. Methods: Linear regression models were developed based on BMI and calf fat and muscle cross-sectional area (FCSA and MCSA) and density measured by pQCT in healthy women (n=76) and men (n=82) aged 20-59 years. Independent variables for these models were leg and total bone-free lean mass (BFLM) and fat mass (FM) measured by DXA. Results:Sex differences (p Conclusion: Calf muscle and fat area and density independently predict lean and fat tissue mass

Validity of Wrist-worn Physical Activity Monitors to Measure Heart Rate

Numerous physical activity monitors exist and are used to track and improve fitness levels. Due to the increasing popularity of these devices, newer products have been developed that measure heart rate (HR) at the wrist. Little is known about how accurate these devices are at measuring HR at the wrist and how they compare to each other. PURPOSE: To determine how accurately HR was measured by three different wrist-worn physical activity monitors. METHODS: Recreationally active men (n=9) and women (n=3) participated in this study. The average age and weight of participants was 22 ± 3 years and 73.9 ± 12 kg. TomTom Cardio (TT), Fitbit Surge (FB) and Microsoft Band (MB) physical activity monitors were used. The TT, FB, and MB were randomly assigned to the right or left wrist for each participant. The testing procedure included speeds of 2, 3, 4, 5, and 6 mph with each speed lasting three minutes. HR was measured by electrocardiography (ECG) using standard limb lead II and by the three different physical activity monitors. HR was recorded from each device every minute throughout the duration of the procedure. Pearson product moment correlations and bias between electrocardiography (ECG) and physical activity monitors with 95% limits of agreement (Bland-Altman analysis) were calculated. Repeated measures ANOVA [Speed x Device] were also calculated. Statistical significance was set at pRESULTS: At 2 mph and 3 mph, only TT HR was significantly correlated with ECG heart rate (r=0.693, p=0.012 and r=0.592, p=0.043). At 4 mph and 6 mph TT was significantly correlated with ECG (r=0.911, pCONCLUSION: With increasing speeds, physical activity monitors more accurately measure HR but individuals should be aware that these devices may overestimate HR during slower walking speeds

Validity of wrist-worn consumer products to measure heart rate and energy expenditure

Introduction: The ability to monitor physical activity throughout the day and during various activities continues to improve with the development of wrist-worn monitors. However, the accuracy of wrist-worn monitors to measure both heart rate and energy expenditure during physical activity is still unclear. The purpose of this study was to determine the accuracy of several popular wrist-worn monitors at measuring heart rate and energy expenditure. Methods: Participants wore the TomTom Cardio, Microsoft Band and Fitbit Surge on randomly assigned locations on each wrist. The maximum number of monitors per wrist was two. The criteria used for heart rate and energy expenditure were a three-lead electrocardiogram and indirect calorimetry using a metabolic cart. Participants exercised on a treadmill at 3.2, 4.8, 6.4, 8 and 9.7 km/h for 3 minutes at each speed, with no rest between speeds. Heart rate and energy expenditure were manually recorded every minute throughout the protocol. Results: Mean absolute percentage error for heart rate varied from 2.17 to 8.06% for the Fitbit Surge, from 1.01 to 7.49% for the TomTom Cardio and from 1.31 to 7.37% for the Microsoft Band. The mean absolute percentage error for energy expenditure varied from 25.4 to 61.8% for the Fitbit Surge, from 0.4 to 26.6% for the TomTom Cardio and from 1.8 to 9.4% for the Microsoft Band. Conclusion: Data from these devices may be useful in obtaining an estimate of heart rate for everyday activities and general exercise, but energy expenditure from these devices may be significantly over- or underestimated

Fatty Acid Methyl Esters as Biosolvents of Epoxy Resins: A Physicochemical Study

The C8 to C18 fatty acid methyl esters (FAME) have been compared as solvents for two epoxy resin pre-polymers, bisphenol A diglycidyl ether (DGEBA) and triglycidyl paminophenol ether (TGPA). It was found that the solubilization limits vary according to the ester and that methyl caprylate is the best solvent of both resins. To explain these solubility performances, physical and chemical properties of FAME were studied, such as the Hansen parameters, viscosity, binary diffusion coefficient and vaporization enthalpy. Determination of the physicochemical parameters of FAME was carried out by laboratory experimentations and by calculation from bibliographic data. The Hansen parameters of FAME and epoxy resins pre-polymers were theoretically and experimentally determined. The FAME chain length showed a long dependence on the binary diffusion parameters and kinematic viscosity, which are mass and momentum transport properties. Moreover, the vaporization enthalpy of these compounds was directly correlated with the solubilization limits

To play or not to play: Can an instrument really impact lip and tongue performance?

© 2020 by the authors. Licensee MDPI, Basel, Switzerland. (1) Background: Increasing tongue and lip strength may help improve various speech and swallowing disorders, but it is unclear if instrumentalists who use these muscle groups for long periods of time have greater strength and endurance compared to controls. It is also unclear if instrumentalists can more accurately estimate various exercise intensities. The purpose of this study was to determine differences in lip and tongue strength and endurance between instrumentalists and non-instrumentalists (controls). A secondary purpose was to assess differences in ability to estimate various exercise intensities between the two groups. (2) Methods: Instrumentalists and controls’ maximum strength and endurance were measured using the IOPI Pro medical device. In addition, 40%, 60% and 80% of maximum strength were estimated in a randomized order. (3) Results: No significant differences were found between instrumentalists and controls in strength or endurance or the ability to estimate various intensities. Overall, participants were better at estimating tongue strength at moderate intensities and lip strength at higher intensities. (4) Conclusion: Tongue and lip strength and endurance and the ability to estimate exercise intensities are not impacted by years of instrumentalist training compared to healthy controls

Impact of Disease Management on Utilization and Adherence With Drugs and Tests: The case of diabetes treatment in the Florida: A Healthy State (FAHS) program

OBJECTIVE—The purpose of this study was to evaluate the effect of telephonic care management within a diabetes disease management program on adherence to treatment with hypoglycemic agents, ACE inhibitors (ACEIs), angiotensin receptor blockers (ARBs), statins, and recommended laboratory tests in a Medicaid population

Lipase-catalysed acylation of starch and determination of the degree of substitution by methanolysis and GC

Background: Natural polysaccharides such as starch are becoming increasingly interesting as renewable starting materials for the synthesis of biodegradable polymers using chemical or enzymatic methods. Given the complexity of polysaccharides, the analysis of reaction products is challenging. Results: Esterification of starch with fatty acids has traditionally been monitored by saponification and back-titration, but in our experience this method is unreliable. Here we report a novel GC-based method for the fast and reliable quantitative determination of esterification. The method was used to monitor the enzymatic esterification of different starches with decanoic acid, using lipase from Thermomyces lanuginosus. The reaction showed a pronounced optimal water content of 1.25 mL per g starch, where a degree of substitution (DS) of 0.018 was obtained. Incomplete gelatinization probably accounts for lower conversion with less water. Conclusions: Lipase-catalysed esterification of starch is feasible in aqueous gel systems, but attention to analytical methods is important to obtain correct DS values

Compliance and persistence with osteoporosis medications: A critical review of the literature

It is widely acknowledged that compliance and persistence with oral osteoporosis medications, particularly with bisphosphonates, is poor. Several excellent reviews have been written on compliance and persistence with osteoporosis medications and have discussed improvements seen with extended dosing intervals. This review begins with studies on extended dosing intervals to examine the limitations of administrative claims data. It also looks at compliance and persistence across multiple medical conditions, examining the importance of prescription fulfillment, intentional choice, causation and possible interventions