Expression of neuropsin in oligodendrocytes after injury to the CNS

Elsevier, Xiao-Ping He, Sadao Shiosaka and Shigetaka Yoshida, Neuroscience Research, 39(4), 2001, 455-462. authorProteases are involved in a variety of processes including demyelination after injury to the central nervous systyem. Neuropsin is a serine protease, which is constitutively expressed in the neurons of the limbic system. In the present study, intrahippocampal kainate injection and enucleation were performed on adult mice. Neuropsin mRNA and protein expression was detected by in situ hybridization and immunohistochemistry. Double in situ hybridization confirmed that the mRNA expression was induced in oligodendrocytes. One day after kainate injection to the hippocampus, neuropsin mRNA was expressed, peaking 4–8 days postoperatively and disappearing at 14 days. Immunohistochemistry and immunoelectron microscopy revealed that neuropsin was expressed in the cell body of oligodendrocytes and myelin. To see if neuropsin degrades myelin protein, purified myelin was incubated with recombinant neuropsin. A decrease in the intensity of the bands of myelin basic protein was observed. These results indicate that neuropsin is involved in demyelination

Alternative Design Comparative System in Collaborative Design

The evaluation of alternative design sets is important phase for quick design decision and new design conception, and it is repeatedly processed. To proceed design process in standard, the repetition is reduced to minimum. And design must be optimized in limited cost and time. For smooth and fast process lots of alternative design sets are provided within the limit of possibility and it must be evaluated appropriately. It's possible to evaluate alternative design sets using various media. This paper describes the characteristics of various media which have been used for evaluation of alternative design sets, and propose alternative design comparative system based on the findings of case studies