The tailless Ortholog nhr-67 Regulates Patterning of Gene Expression and Morphogenesis in the C. elegans Vulva

Regulation of spatio-temporal gene expression in diverse cell and tissue types is a critical aspect of development. Progression through Caenorhabditis elegans vulval development leads to the generation of seven distinct vulval cell types (vulA, vulB1, vulB2, vulC, vulD, vulE, and vulF), each with its own unique gene expression profile. The mechanisms that establish the precise spatial patterning of these mature cell types are largely unknown. Dissection of the gene regulatory networks involved in vulval patterning and differentiation would help us understand how cells generate a spatially defined pattern of cell fates during organogenesis. We disrupted the activity of 508 transcription factors via RNAi and assayed the expression of ceh-2, a marker for vulB fate during the L4 stage. From this screen, we identified the tailless ortholog nhr-67 as a novel regulator of gene expression in multiple vulval cell types. We find that one way in which nhr-67 maintains cell identity is by restricting inappropriate cell fusion events in specific vulval cells, namely vulE and vulF. nhr-67 exhibits a dynamic expression pattern in the vulval cells and interacts with three other transcriptional regulators cog-1 (Nkx6.1/6.2), lin-11 (LIM), and egl-38 (Pax2/5/8) to generate the composite expression patterns of their downstream targets. We provide evidence that egl-38 regulates gene expression in vulB1, vulC, vulD, vulE, as well as vulF cells. We demonstrate that the pairwise interactions between these regulatory genes are complex and vary among the seven cell types. We also discovered a striking regulatory circuit that affects a subset of the vulval lineages: cog-1 and nhr-67 inhibit both one another and themselves. We postulate that the differential levels and combinatorial patterns of lin-11, cog-1, and nhr-67 expression are a part of a regulatory code for the mature vulval cell types

Specific Immune Response of Mares and their Newborn Foals to Actinobacillus spp. Present in the Oral Cavity

Oral swab samples, serum and colostrum was taken from 15 mares and 14 of their foals, within 24 h of birth. The presence of antibody against Actinobacillus spp. isolated from the oral cavity was investigated using agar gel immunodiffusion. Antibodies against 48 out of the 77 Actinobacillus isolates from all horses in the study were present in the respective sera of 13 mares and 9 foals. In 11 mother-foal pairs, the antibody content of the foal serum was similar to that of the mare, and in 9 cases this was reflected in the antibody content of colostrum from the mare. The results indicate that an immune response to Actinobacillus spp. colonising the oral cavity is present in many adult horses and that this immune response can be transferred from mother to foal via colostrum

MIDAS prototype Multispectral Interactive Digital Analysis System for large area earth resources surveys. Volume 2: Charge coupled device investigation

MIDAS is a third-generation, fast, low cost, multispectral recognition system able to keep pace with the large quantity and high rates of data acquisition from large regions with present and projected sensors. MIDAS, for example, can process a complete ERTS frame in forty seconds and provide a color map of sixteen constituent categories in a few minutes. A principal objective of the MIDAS Program is to provide a system well interfaced with the human operator and thus to obtain large overall reductions in turn-around time and significant gains in throughput. The need for advanced onboard spacecraft processing of remotely sensed data is stated and approaches to this problem are described which are feasible through the use of charge coupled devices. Tentative mechanizations for the required processing operations are given in large block form. These initial designs can serve as a guide to circuit/system designers

The lin-15 locus encodes two negative regulators of Caenorhabditis elegans vulval development

During Caenorhabditis elegans vulval development, an inductive signal from the anchor cell stimulates three of the six vulval precursor cells (VPCs) to adopt vulval rather than nonvulval epidermal fates. Genes necessary for this induction include the lin-3 growth factor, the let-23 receptor tyrosine kinase, and let-60 ras. lin-15 is a negative regulator of this inductive pathway. In lin-15 mutant animals, all six VPCs adopt vulval fates, even in the absence of inductive signal. Previous genetic studies suggested that lin-15 is a complex locus with two independently mutable activities, A and B. We have cloned the lin-15 locus by germline transformation and find that it encodes two nonoverlapping transcripts that are transcribed in the same direction. The downstream transcript encodes the lin-15A function; the upstream transcript encodes the lin-15B function. The predicted lin-15A and lin- 15B proteins are novel and hydrophilic. We have identified a molecular null allele of lin-15 and have used it to analyze the role of lin-15 in the signaling pathway. We find that lin-15 acts upstream of let-23 and in parallel to the inductive signal

On pseudo-hyperk\"ahler prepotentials

An explicit surjection from a set of (locally defined) unconstrained holomorphic functions on a certain submanifold of (Sp_1(C) \times C^{4n}) onto the set HK_{p,q} of local isometry classes of real analytic pseudo-hyperk\"ahler metrics of signature (4p,4q) in dimension 4n is constructed. The holomorphic functions, called prepotentials, are analogues of K\"ahler potentials for K\"ahler metrics and provide a complete parameterisation of HK_{p,q}. In particular, there exists a bijection between HK_{p,q} and the set of equivalence classes of prepotentials. This affords the explicit construction of pseudo-hyperk\"ahler metrics from specified prepotentials. The construction generalises one due to Galperin, Ivanov, Ogievetsky and Sokatchev. Their work is given a coordinate-free formulation and complete, self-contained proofs are provided. An appendix provides a vital tool for this construction: a reformulation of real analytic G-structures in terms of holomorphic frame fields on complex manifolds.Comment: 53 pages; v2: minor amendments to Def.4.1 and Theorem 4.5; a paragraph inserted in the proof of the latter; V3: minor changes; V4: minor changes/ typos corrected for journal versio

On the Composition of Gauge Structures

A formulation for a non-trivial composition of two classical gauge structures is given: Two parent gauge structures of a common base space are synthesized so as to obtain a daughter structure which is fundamental by itself. The model is based on a pair of related connections that take their values in the product space of the corresponding Lie algebras. The curvature, the covariant exterior derivatives and the associated structural identities, all get contributions from both gauge groups. The various induced structures are classified into those whose composition is given just by trivial means, and those which possess an irreducible nature. The pure irreducible piece, in particular, generates a complete super-space of ghosts with an attendant set of super-BRST variation laws, both of which are purely of a geometrical origin.Comment: Few elaborations are added to section 4 and section 5. To be published in Journal of Physics A: Mathematical and General. 21 page