209 research outputs found
Recommended from our members
Low energy electron attachment to condensed formic acid
Dissociative electron attachment to formic acid in the condensed phase is studied using improved mass spectrometric detection of the negative ion fragments. The desorbed yields are measured as a function of incident electron energy in the range between 3 to 20 eV. Unlike previous work, the formation of the dehydrogenated anion HCOO? is observed and the signal to noise ratio is much higher for all other ions detected, i.e. OH?, O? and H?. Resonant structure seen in all anion yield functions, is attributed to dissociative electron attachment (DEA), whereas above 14 eV nonresonant dipolar dissociation (DD) dominates the desorption yields
RUNX1/ETO blocks selectin-mediated adhesion via epigenetic silencing of PSGL-1
RUNX1/ETO (RE),the t(8;21)-derived leukemic transcription factor associated with acute myeloid leukemia (AML) development, deregulates genes involved in differentiation, self-renewal and proliferation. In addition, these cells show differences in cellular adhesion behavior whose molecular basis is not well understood. Here, we demonstrate that RE epigenetically silences the gene encoding P-Selectin Glycoprotein Ligand-1 (PSGL-1) and downregulates PSGL-1 expression in human CD34+ and murine lin-hematopoietic progenitor cells. Levels of PSGL-1 inversely and dose-dependently correlate with RE oncogene levels. However, a DNA-binding defective mutant fails to downregulate PSGL-1. We show by ChIP experiments that the PSGL-1 promoter is a direct target of RE and binding is accompanied by high levels of the repressive chromatin mark histone H3K27me3. In t(8;21)+ Kasumi-1 cells, PSGL-1 expression is completely restored at both the mRNA and cell surface protein levels following RE downregulation with short hairpin RNA (shRNA) or RE inhibition with tetramerization-blocking peptides, and at the promoter H3K27me3 is replaced by the activating chromatin mark H3K9ac as well as by RNA polymerase II. Upregulation of PSGL-1 restores the binding of cells to P- and E-selectin and re-establishes myeloid-specific cellular adhesion while it fails to bind to lymphocyte-specific L-selectin. Overall, our data suggest that the RE oncoprotein epigenetically represses PSGL-1 via binding to its promoter region and thus affects the adhesive behavior of t(8;21)+ AML cells
Electron scattering from molecules and molecular aggregates of biological relevance
In this Topical Review we survey the current state of the art in the study of low energy electron collisions with biologically relevant molecules and molecular clusters. We briefly describe the methods and techniques used in the investigation of these processes and summarise the results obtained so far for DNA constituents and their model compounds, amino acids, peptides and other biomolecules. The applications of the data obtained is briefly described as well as future required developments
A FOXO1-induced oncogenic network defines the AML1-ETO preleukemic program
Key Points
Increased FOXO1 is oncogenic in human CD34+ cells and promotes preleukemia transition. FOXO1 is required by AE preleukemia cells for the activation of a stem cell molecular program.</jats:p
Recommended from our members
Depletion of RUNX1/ETO in t(8;21) AML cells leads to genome-wide changes in chromatin structure and transcription factor binding
The t(8;21) translocation fuses the DNA-binding domain of the hematopoietic master regulator RUNX1 to the ETO protein. The resultant RUNX1/ETO fusion protein is a leukemia-initiating transcription factor that interferes with RUNX1 function. The result of this interference is a block in differentiation and, finally, the development of acute myeloid leukemia (AML). To obtain insights into RUNX1/ETO-dependant alterations of the epigenetic landscape, we measured genome-wide RUNX1- and RUNX1/ETO-bound regions in t(8;21) cells and assessed to what extent the effects of RUNX1/ETO on the epigenome depend on its continued expression in established leukemic cells. To this end, we determined dynamic alterations of histone acetylation, RNA Polymerase II binding and RUNX1 occupancy in the presence or absence of RUNX1/ETO using a knockdown approach. Combined global assessments of chromatin accessibility and kinetic gene expression data show that RUNX1/ETO controls the expression of important regulators of hematopoietic differentiation and self-renewal. We show that selective removal of RUNX1/ETO leads to a widespread reversal of epigenetic reprogramming and a genome-wide redistribution of RUNX1 binding, resulting in the inhibition of leukemic proliferation and self-renewal, and the induction of differentiation. This demonstrates that RUNX1/ETO represents a pivotal therapeutic target in AML
Electronic structure of silver-bismuth iodide rudorffite nanomaterials studied by synchrotron radiation soft X-ray photoemission spectroscopy
Silver-bismuth iodide (Ag-Bi-I) rudorffites are chemically stable and non-toxic materials that can act as a possible replacement for methylammonium lead halide perovskites in optoelectronic devices. In this report we will present innovative routes for fabrication of AgBi-I nanomaterials, as well as the results of the investigation of the electronic structure of isolated Ag-Bi-I nanoparticles by soft X-ray aerosol photoemission spectroscopy [1, 2]. Aerosol photoemission spectroscopy allows studies of the electronic structure of submicrometer particles that are free from the influence of a substrate or solvent [1-5]. In this approach the aerosol particles can be produced directly from a solution or a colloidal dispersion, which opens a possibility for investigation of a variety of nanosystems that can be produced by wet chemistry methods. This technique relies on the interaction of focused beam of isolated particles with ionizing radiation under high vacuum conditions. In addition, by using tunable synchrotron radiation as an excitation source it is possible to obtain highresolution photoelectron spectra in the investigated photoelectron energy range.X Serbian Ceramic Society Conference - Advanced Ceramics and Application : new frontiers in multifunctional material science and processing : program and the book of abstracts; September 26-27, 2022; Belgrad
RUNX1-ETO Depletion in t(8;21) AML Leads to C/EBP alpha- and AP-1-Mediated Alterations in Enhancer-Promoter Interaction
Acute myeloid leukemia (AML) is associated with mutations in transcriptional and epigenetic regulator
genes impairing myeloid differentiation. The t(8;21)
(q22;q22) translocation generates the RUNX1-ETO
fusion protein, which interferes with the hematopoietic master regulator RUNX1. We previously showed
that the maintenance of t(8;21) AML is dependent on
RUNX1-ETO expression. Its depletion causes extensive changes in transcription factor binding, as well
as gene expression, and initiates myeloid differentiation. However, how these processes are connected
within a gene regulatory network is unclear. To
address this question, we performed Promoter-Capture Hi-C assays, with or without RUNX1-ETO depletion and assigned interacting cis-regulatory elements
to their respective genes. To construct a RUNX1-
ETO-dependent gene regulatory network maintaining
AML, we integrated cis-regulatory element interactions with gene expression and transcription factor
binding data. This analysis shows that RUNX1-ETO
participates in cis-regulatory element interactions.
However, differential interactions following RUNX1-
ETO depletion are driven by alterations in the binding
of RUNX1-ETO-regulated transcription factors
- …