100 research outputs found
ΠΡΠ΅Π½ΠΊΠ° Π½Π΅ΡΠ°Π²Π½ΠΎΠΌΠ΅ΡΠ½ΠΎΡΡΠΈ ΡΠΏΡΡΠ³ΠΈΡ ΡΠ²ΠΎΠΉΡΡΠ² Π»ΠΈΡΡΠΎΠ² ΠΈΠ· Π·Π°ΠΊΡΡΡΠΎΡΡΠ΅ΠΈΡΡΡΡ ΠΏΠ΅Π½ΠΎΠΏΠΎΠ»ΠΈΠΎΠ»Π΅ΡΠΈΠ½ΠΎΠ² Π°ΠΊΡΡΡΠΈΡΠ΅ΡΠΊΠΈΠΌ ΠΌΠ΅ΡΠΎΠ΄ΠΎΠΌ
The widespread use of polyolefin foams in strategically important industries is due to their high thermal, sound and vibration insulation properties. The aim of the work was to evaluate the non-uniformity of elastic properties over the area of sheets of polyolefin foams of various types using the acoustic non-contact shadow amplitude method of testing and confirmation by the structural analysis method.The article presents the developed installation and a new method of non-contact acoustic testing of sheets made of closed-cell polyolefin foams based on recording the amplitude of the pulse that passed through the sheet and allowing to assess to the unevenness of its elastic properties during scanning. Studies of uneven elastic properties were carried out on sheets of closed-cell polyolefin foams of the ISOLON 500 and ISOLON 300 brands which differ in material and manufacturing technology (technique of cross-linking, method and multiplicity of foaming).It is shown that the absolute amplitude of the signal and its spread relative to the average value is affected by the structure of the foam polyolefin material and its heterogeneity over the area of the studied sheet determined by the production technology which is confirmed visually using microscopy.Studies have shown the effect on the indications unevenness of the method of obtaining and the apparent density of the material. It is shown that the most uneven elastic properties and structure belong to sheets of polyolefin foam obtained by chemical cross-linking technology (the unevenness of Ξ was 6.5 %). Among the physically cross-linked sheets of polyolefin foam the most uniform in structure and elastic properties are samples made of ethylene vinyl acetate with Ξ = 3.8 %, as well as sheets with a high foaming rate (Ξ = 3.9 %). The unevenness of structure of the studied sheets of polyolefin foams was confirmed by optical microscopy of sections in two mutually perpendicular directions.Π¨ΠΈΡΠΎΠΊΠΎΠ΅ ΠΈΡΠΏΠΎΠ»ΡΠ·ΠΎΠ²Π°Π½ΠΈΠ΅ ΠΏΠ΅Π½ΠΎΠΏΠΎΠ»ΠΈΠΎΠ»Π΅ΡΠΈΠ½ΠΎΠ² Π² ΡΡΡΠ°ΡΠ΅Π³ΠΈΡΠ΅ΡΠΊΠΈ Π²Π°ΠΆΠ½ΡΡ
ΠΎΡΡΠ°ΡΠ»ΡΡ
ΠΏΡΠΎΠΌΡΡΠ»Π΅Π½Π½ΠΎΡΡΠΈ ΠΎΠ±ΡΡΠ»ΠΎΠ²Π»Π΅Π½ΠΎ ΠΈΡ
Π²ΡΡΠΎΠΊΠΈΠΌΠΈ ΡΠ΅ΠΏΠ»ΠΎ-, Π·Π²ΡΠΊΠΎ- ΠΈ Π²ΠΈΠ±ΡΠΎΠΈΠ·ΠΎΠ»ΡΡΠΈΠΎΠ½Π½ΡΠΌΠΈ ΡΠ²ΠΎΠΉΡΡΠ²Π°ΠΌΠΈ. Π¦Π΅Π»ΡΡ ΡΠ°Π±ΠΎΡΡ ΡΠ²Π»ΡΠ»Π°ΡΡ ΠΎΡΠ΅Π½ΠΊΠ° Π½Π΅ΡΠ°Π²Π½ΠΎΠΌΠ΅ΡΠ½ΠΎΡΡΠΈ ΡΠΏΡΡΠ³ΠΈΡ
ΡΠ²ΠΎΠΉΡΡΠ² ΠΏΠΎ ΠΏΠ»ΠΎΡΠ°Π΄ΠΈ Π»ΠΈΡΡΠΎΠ² ΠΏΠ΅Π½ΠΎΠΏΠΎΠ»ΠΈΠΎΠ»Π΅ΡΠΈΠ½ΠΎΠ² ΡΠ°Π·Π»ΠΈΡΠ½ΡΡ
ΡΠΈΠΏΠΎΠ² Ρ ΠΈΡΠΏΠΎΠ»ΡΠ·ΠΎΠ²Π°Π½ΠΈΠ΅ΠΌ Π°ΠΊΡΡΡΠΈΡΠ΅ΡΠΊΠΎΠ³ΠΎ Π±Π΅ΡΠΊΠΎΠ½ΡΠ°ΠΊΡΠ½ΠΎΠ³ΠΎ ΡΠ΅Π½Π΅Π²ΠΎΠ³ΠΎ Π°ΠΌΠΏΠ»ΠΈΡΡΠ΄Π½ΠΎΠ³ΠΎ ΠΌΠ΅ΡΠΎΠ΄Π° ΠΊΠΎΠ½ΡΡΠΎΠ»Ρ ΠΈ ΠΏΠΎΠ΄ΡΠ²Π΅ΡΠΆΠ΄Π΅Π½ΠΈΠ΅ΠΌ ΠΌΠ΅ΡΠΎΠ΄ΠΎΠΌ ΡΡΡΡΠΊΡΡΡΠ½ΠΎΠ³ΠΎ Π°Π½Π°Π»ΠΈΠ·Π°.Π Π°Π·ΡΠ°Π±ΠΎΡΠ°Π½Ρ ΡΡΡΠ°Π½ΠΎΠ²ΠΊΠ° ΠΈ Π½ΠΎΠ²Π°Ρ ΠΌΠ΅ΡΠΎΠ΄ΠΈΠΊΠ° Π±Π΅ΡΠΊΠΎΠ½ΡΠ°ΠΊΡΠ½ΠΎΠ³ΠΎ Π°ΠΊΡΡΡΠΈΡΠ΅ΡΠΊΠΎΠ³ΠΎ ΠΊΠΎΠ½ΡΡΠΎΠ»Ρ Π»ΠΈΡΡΠΎΠ² ΠΈΠ· Π·Π°ΠΊΡΡΡΠΎΡΡΠ΅ΠΈΡΡΡΡ
ΠΏΠ΅Π½ΠΎΠΏΠΎΠ»ΠΈΠΎΠ»Π΅ΡΠΈΠ½ΠΎΠ², ΠΎΡΠ½ΠΎΠ²Π°Π½Π½Π°Ρ Π½Π° ΡΠ΅Π³ΠΈΡΡΡΠ°ΡΠΈΠΈ Π°ΠΌΠΏΠ»ΠΈΡΡΠ΄Ρ ΠΈΠΌΠΏΡΠ»ΡΡΠ°, ΠΏΡΠΎΡΠ΅Π΄ΡΠ΅Π³ΠΎ ΡΠΊΠ²ΠΎΠ·Ρ Π»ΠΈΡΡ, ΠΈ ΠΏΠΎΠ·Π²ΠΎΠ»ΡΡΡΠ°Ρ ΠΎΡΠ΅Π½ΠΈΡΡ Π½Π΅ΡΠ°Π²Π½ΠΎΠΌΠ΅ΡΠ½ΠΎΡΡΡ Π΅Π³ΠΎ ΡΠΏΡΡΠ³ΠΈΡ
ΡΠ²ΠΎΠΉΡΡΠ² Π² ΠΏΡΠΎΡΠ΅ΡΡΠ΅ ΡΠΊΠ°Π½ΠΈΡΠΎΠ²Π°Π½ΠΈΡ. ΠΡΡΠ»Π΅Π΄ΠΎΠ²Π°Π½ΠΈΡ Π½Π΅ΡΠ°Π²Π½ΠΎΠΌΠ΅ΡΠ½ΠΎΡΡΠΈ ΡΠΏΡΡΠ³ΠΈΡ
ΡΠ²ΠΎΠΉΡΡΠ² ΠΏΡΠΎΠ²Π΅Π΄Π΅Π½Ρ Π½Π° Π»ΠΈΡΡΠ°Ρ
ΠΈΠ· Π·Π°ΠΊΡΡΡΠΎΡΡΠ΅ΠΈΡΡΡΡ
ΠΏΠ΅Π½ΠΎΠΏΠΎΠ»ΠΈΠΎΠ»Π΅ΡΠΈΠ½ΠΎΠ² ΠΌΠ°ΡΠΊΠΈ ISOLON 500 ΠΈ ISOLON 300, ΡΠ°Π·Π»ΠΈΡΠ°ΡΡΠΈΠ΅ΡΡ ΠΌΠ°ΡΠ΅ΡΠΈΠ°Π»ΠΎΠΌ ΠΈ ΡΠ΅Ρ
Π½ΠΎΠ»ΠΎΠ³ΠΈΠ΅ΠΉ ΠΈΠ·Π³ΠΎΡΠΎΠ²Π»Π΅Π½ΠΈΡ (ΡΠΏΠΎΡΠΎΠ± ΡΡΠΈΠ²ΠΊΠΈ, ΠΌΠ΅ΡΠΎΠ΄ ΠΈ ΠΊΡΠ°ΡΠ½ΠΎΡΡΡ Π²ΡΠΏΠ΅Π½ΠΈΠ²Π°Π½ΠΈΡ).ΠΠΎΠΊΠ°Π·Π°Π½ΠΎ, ΡΡΠΎ Π½Π° Π°Π±ΡΠΎΠ»ΡΡΠ½ΡΡ Π°ΠΌΠΏΠ»ΠΈΡΡΠ΄Ρ ΡΠΈΠ³Π½Π°Π»Π° ΠΈ Π΅Ρ ΡΠ°Π·Π±ΡΠΎΡ ΠΎΡΠ½ΠΎΡΠΈΡΠ΅Π»ΡΠ½ΠΎ ΡΡΠ΅Π΄Π½Π΅Π³ΠΎ Π·Π½Π°ΡΠ΅Π½ΠΈΡ Π²Π»ΠΈΡΠ΅Ρ ΡΡΡΡΠΊΡΡΡΠ° ΠΌΠ°ΡΠ΅ΡΠΈΠ°Π»Π° ΠΏΠ΅Π½ΠΎΠΏΠΎΠ»ΠΈΠΎΠ»Π΅ΡΠΈΠ½Π° ΠΈ Π΅Ρ Π½Π΅ΠΎΠ΄Π½ΠΎΡΠΎΠ΄Π½ΠΎΡΡΡ ΠΏΠΎ ΠΏΠ»ΠΎΡΠ°Π΄ΠΈ ΠΈΡΡΠ»Π΅Π΄ΡΠ΅ΠΌΠΎΠ³ΠΎ Π»ΠΈΡΡΠ°, ΠΎΠΏΡΠ΅Π΄Π΅Π»ΡΠ΅ΠΌΠ°Ρ ΡΠ΅Ρ
Π½ΠΎΠ»ΠΎΠ³ΠΈΠ΅ΠΉ ΠΏΡΠΎΠΈΠ·Π²ΠΎΠ΄ΡΡΠ²Π°, ΡΡΠΎ ΠΏΠΎΠ΄ΡΠ²Π΅ΡΠΆΠ΄Π΅Π½ΠΎ Π²ΠΈΠ·ΡΠ°Π»ΡΠ½ΠΎ Ρ ΠΈΡΠΏΠΎΠ»ΡΠ·ΠΎΠ²Π°Π½ΠΈΠ΅ΠΌ ΠΌΠΈΠΊΡΠΎΡΠΊΠΎΠΏΠΈΠΈ. ΠΡΡΠ»Π΅Π΄ΠΎΠ²Π°Π½ΠΈΡ ΠΏΠΎΠΊΠ°Π·Π°Π»ΠΈ Π²Π»ΠΈΡΠ½ΠΈΠ΅ Π½Π° Π½Π΅ΡΠ°Π²Π½ΠΎΠΌΠ΅ΡΠ½ΠΎΡΡΡ ΠΏΠΎΠΊΠ°Π·Π°Π½ΠΈΠΉ ΡΠΏΠΎΡΠΎΠ±Π° ΠΏΠΎΠ»ΡΡΠ΅Π½ΠΈΡ ΠΈ ΠΊΠ°ΠΆΡΡΠ΅ΠΉΡΡ ΠΏΠ»ΠΎΡΠ½ΠΎΡΡΠΈ ΠΌΠ°ΡΠ΅ΡΠΈΠ°Π»Π°. ΠΠΎΠΊΠ°Π·Π°Π½ΠΎ, ΡΡΠΎ Π½Π°ΠΈΠ±ΠΎΠ»Π΅Π΅ Π½Π΅ΡΠ°Π²Π½ΠΎΠΌΠ΅ΡΠ½ΡΠ΅ ΡΠΏΡΡΠ³ΠΈΠ΅ ΡΠ²ΠΎΠΉΡΡΠ²Π° ΠΈ ΡΡΡΡΠΊΡΡΡΡ ΠΈΠΌΠ΅ΡΡ Π»ΠΈΡΡΡ ΠΈΠ· ΠΏΠ΅Π½ΠΎΠΏΠΎΠ»ΠΈΠΎΠ»Π΅ΡΠΈΠ½ΠΎΠ², ΠΏΠΎΠ»ΡΡΠ΅Π½Π½ΡΡ
ΠΏΠΎ ΡΠ΅Ρ
Π½ΠΎΠ»ΠΎΠ³ΠΈΠΈ Ρ
ΠΈΠΌΠΈΡΠ΅ΡΠΊΠΎΠΉ ΡΡΠΈΠ²ΠΊΠΈ (Π½Π΅ΡΠ°Π²Π½ΠΎΠΌΠ΅ΡΠ½ΠΎΡΡΡ Ξ ΡΠΎΡΡΠ°Π²ΠΈΠ»Π° 6,5 %). ΠΠ· ΡΠΈΠ·ΠΈΡΠ΅ΡΠΊΠΈ ΡΡΠΈΡΡΡ
Π»ΠΈΡΡΠΎΠ² ΠΏΠ΅Π½ΠΎΠΏΠΎΠ»ΠΈΠΎΠ»Π΅ΡΠΈΠ½ΠΎΠ² Π½Π°ΠΈΠ±ΠΎΠ»Π΅Π΅ ΡΠ°Π²Π½ΠΎΠΌΠ΅ΡΠ½ΡΠΌΠΈ ΠΏΠΎ ΡΡΡΡΠΊΡΡΡΠ΅ ΠΈ ΡΠΏΡΡΠ³ΠΈΠΌ ΡΠ²ΠΎΠΉΡΡΠ²Π°ΠΌ ΡΠ²Π»ΡΡΡΡΡ ΠΎΠ±ΡΠ°Π·ΡΡ, ΠΈΠ·Π³ΠΎΡΠΎΠ²Π»Π΅Π½Π½ΡΠ΅ ΠΈΠ· ΡΡΠΈΠ»Π΅Π½Π²ΠΈΠ½ΠΈΠ»Π°ΡΠ΅ΡΠ°ΡΠ° Ρ Ξ = 3,8 %, Π° ΡΠ°ΠΊΠΆΠ΅ Π»ΠΈΡΡΡ Ρ Π²ΡΡΠΎΠΊΠΎΠΉ ΠΊΡΠ°ΡΠ½ΠΎΡΡΡΡ Π²ΡΠΏΠ΅Π½ΠΈΠ²Π°Π½ΠΈΡ (Ξ = 3,9 %). ΠΠ΅ΡΠ°Π²Π½ΠΎΠΌΠ΅ΡΠ½ΠΎΡΡΡ ΡΡΡΡΠΊΡΡΡΡ ΠΈΡΡΠ»Π΅Π΄ΠΎΠ²Π°Π½Π½ΡΡ
Π»ΠΈΡΡΠΎΠ² ΠΏΠ΅Π½ΠΎΠΏΠΎΠ»ΠΈΠΎΠ»Π΅ΡΠΈΠ½ΠΎΠ² ΠΏΠΎΠ΄ΡΠ²Π΅ΡΠΆΠ΄Π΅Π½Π° ΠΎΠΏΡΠΈΡΠ΅ΡΠΊΠΎΠΉ ΠΌΠΈΠΊΡΠΎΡΠΊΠΎΠΏΠΈΠ΅ΠΉ ΡΡΠ΅Π·ΠΎΠ² Π² Π΄Π²ΡΡ
Π²Π·Π°ΠΈΠΌΠ½ΠΎ ΠΏΠ΅ΡΠΏΠ΅Π½Π΄ΠΈΠΊΡΠ»ΡΡΠ½ΡΡ
Π½Π°ΠΏΡΠ°Π²Π»Π΅Π½ΠΈΡΡ
QM/MM description of newly selected catalytic bioscavengers against organophosphorus compounds revealed reactivation stimulus mediated by histidine residue in the acyl-binding loop
Β© 2018 Zlobin, Mokrushina, Terekhov, Zalevsky, Bobik, Stepanova, Aliseychik, Kartseva, Panteleev, Golovin, Belogurov, Gabibov and Smirnov. Butyrylcholinesterase (BChE) is considered as an efficient stoichiometric antidote against organophosphorus (OP) poisons. Recently we utilized combination of calculations and ultrahigh-throughput screening (uHTS) to select BChE variants capable of catalytic destruction of OP pesticide paraoxon. The purpose of this study was to elucidate the molecular mechanism underlying enzymatic hydrolysis of paraoxon by BChE variants using hybrid quantum mechanical/molecular mechanical (QM/MM) calculations. Detailed analysis of accomplished QM/MM runs revealed that histidine residues introduced into the acyl-binding loop are always located in close proximity with aspartate residue at position 70. Histidine residue acts as general base thus leading to attacking water molecule activation and subsequent SN2 inline hydrolysis resulting in BChE reactivation. This combination resembles canonical catalytic triad found in active centers of various proteases. Carboxyl group activates histidine residue by altering its pKa, which in turn promotes the activation of water molecule in terms of its nucleophilicity. Observed re-protonation of catalytic serine residue at position 198 from histidine residue at position 438 recovers initial configuration of the enzyme's active center, facilitating next catalytic cycle. We therefore suggest that utilization of uHTS platform in combination with deciphering of molecular mechanisms by QM/MM calculations may significantly improve our knowledge of enzyme function, propose new strategies for enzyme design and open new horizons in generation of catalytic bioscavengers against OP poisons
Reconstruction of recombination sites in genomic structures of the strains of genotype 6 of hepatitis C virus
The encoded portion of the complete genomes of 46 strains of the genotype 6 of hepatitis C virus through bioinformatics RDP programs complex group of 6 recombinants strains was identified, in which 7 recombination sites were fixed. Strains correspond to the three-recombinant HCV subtypes: 6a, 6b and 61. For each of the identified recombinant we defined parent strains from which they can be obtained. Three recombinants were obtained from parent strains of the same subtype (homologous inside subgenotypic recombination). For the remaining three recombinants parent strains were members of three different subtypes (between subgenotypic recombination).In one strain we identified a unique recombination site in a highly conservative NS3 gene. Most of the recombination sites occurred in the region of the structural genes C, E1 and E2, and in the area of non-structural genes NS5a and NS5b.In the recombinant strain DQ480518-6a two recombination site were identified. One site is located in the structural and nonstructural genes (E2 + NS1 + NS2), and a second one in non-structural region. Dimensions of recombination sites can vary from 86 to 1072 nucleotide bases. The study identified "hot spots" of recombination in the strains of genotype 6 of hepatitis C virus. The recombinants were found in the population of the three countries: the United States (from the serum of an immigrant), Hong Kong and China
The groups of patients by genetic research for sensibility to Dalcetrapib therapy
The article deals with the search for phenotypic differences between patients with genotypically determined susceptibility to the drug Dalcetrapib and without it.Π ΡΡΠ°ΡΡΠ΅ ΡΠ°ΡΡΠΌΠΎΡΡΠ΅Π½Ρ Π²ΠΎΠΏΡΠΎΡΡ ΠΏΠΎΠΈΡΠΊΠ° ΡΠ΅Π½ΠΎΡΠΈΠΏΠΈΡΠ΅ΡΠΊΠΈΡ
ΠΎΡΠ»ΠΈΡΠΈΠΉ ΠΌΠ΅ΠΆΠ΄Ρ ΠΏΠ°ΡΠΈΠ΅Π½ΡΠ°ΠΌΠΈ Ρ Π³Π΅Π½ΠΎΡΠΈΠΏΠΈΡΠ΅ΡΠΊΠΈ ΠΎΠ±ΡΡΠ»ΠΎΠ²Π»Π΅Π½Π½ΠΎΠΉ Π²ΠΎΡΠΏΡΠΈΠΈΠΌΡΠΈΠ²ΠΎΡΡΡΡ ΠΊ ΠΏΡΠ΅ΠΏΠ°ΡΠ°ΡΡ ΠΠ°Π»ΡΠ΅ΡΡΠ°ΠΏΠΈΠ± ΠΈ Π±Π΅Π· Π½Π΅Π΅
CHARACTERISTICS OF THE BAIKAL SUBTYPE OF TICK-BORNE ENCEPHALITIS VIRUS CIRCULATING IN EASTERN SIBERIA
Background. During the study of the genetic variability of the tick-borne encephalitis virus (TBEV) in Eastern Siberia, aΒ group of 22Β strains with a unique genetic structure significantly different from allΒ known TBEV subtypes was identified.Β This TBEV variant wasΒ tentatively called βgroup 886β. Therefore, for this original TBEVΒ variant it was necessary to study theΒ genetic, biological properties of the βgroup 886β strains, clarify its TBEV taxonomic status, its range, evolutionary history, etc.Aim. The generalization of the currently available data on genetic and biological properties of TBEV β886β group.Materials and methods. The genetic structure of βgroup 886β strains was studied by the complex of molecular-geneticΒ methods (MHNA, sequencing of fragments or the complete genome).Results. It was shown that βgroup 886β strains form a separate cluster on phylogenetic tree, and the level of geneticΒ differencesΒ from other genotypes is more than 12 %. It was defined that thisΒ TBEV variant has its own area (IrkutskΒ region, Republic of Buryatia,Β Trans-Baikal region, Northern Mongolia). Its ecological connectionΒ with all links of theΒ transmissive chain (ixodid ticks, small mammals,Β human), participation in human pathology, stability and duration ofΒ Β circulation in the Baikal region, individual evolutionary historyΒ were proved. Some phenotypic characteristics of theΒ βgroup 886β strains were considered.Conclusion. The presented data testify to the validity of the β886 groupβ isolation as an independent genetic type. Taking intoΒ accountΒ the geographical distribution of this TBEV genotype, we propose to assign it the name βBaikal genotype/subtypeβ
The groups of patients by genetic research for sensibility to Dalcetrapib therapy
This review article defines several aspects of phenotypic variations of patients with increased sensitivity to Dalcetrapib therapy and people who doesn't have any genetically-based predisposition to it.Π ΡΡΠ°ΡΡΠ΅ ΡΠ°ΡΡΠΌΠΎΡΡΠ΅Π½Ρ Π²ΠΎΠΏΡΠΎΡΡ ΠΎΠΏΡΠ΅Π΄Π΅Π»Π΅Π½ΠΈΡ ΡΠ΅Π½ΠΎΡΠΈΠΏΠΈΡΠ΅ΡΠΊΠΈΡ
ΠΎΡΠ»ΠΈΡΠΈΠΉ ΠΏΠ°ΡΠΈΠ΅Π½ΡΠΎΠ² Ρ ΠΏΠΎΠ²ΡΡΠ΅Π½Π½ΠΎΠΉ Π²ΠΎΡΠΏΡΠΈΠΈΠΌΡΠΈΠ²ΠΎΡΡΡΡ ΠΊ ΠΏΡΠ΅ΠΏΠ°ΡΠ°ΡΡ ΠΠ°Π»ΡΠ΅ΡΡΠ°ΠΏΠΈΠ± ΠΈ Π»ΡΠ΄Π΅ΠΉ, Π½Π΅ ΠΈΠΌΠ΅ΡΡΠΈΡ
Π³Π΅Π½ΠΎΡΠΈΠΏΠΈΡΠ΅ΡΠΊΡΡ ΠΏΡΠ΅Π΄ΡΠ°ΡΠΏΠΎΠ»ΠΎΠΆΠ΅Π½Π½ΠΎΡΡΡ ΠΊ Π΄Π°Π½Π½ΠΎΠΌΡ ΠΏΡΠ΅ΠΏΠ°ΡΠ°ΡΡ
Characteristics of genetic and phenotypic properties of tick-borne encephalitis virus strains isolated from various source on the territory of Eastern Siberia
Since ixodid ticks and their feeders (vertebrates) form the basis of the tick-borne encephalitis virus (TBEV) population, it was interesting to obtain a genetic characteristic of strains isolated from various sources in the territory of Eastern Siberia. In our study, it was found that the circulation of TBEV of genotypes 1, 2, 3, 5 and "polytypic" strains in the territory of Eastern Siberia is maintained both by the main vectors (I. persulcatus ticks) and by their feeders (small and large mammals, birds). The distribution of genotypes in strains groups from ixodid ticks and vertebrates varied. TBEV strains of genotypes 1 and 2 were significantly more often isolated from rodents, and genotype 3 from ixodid ticks (p < 0.05). Isolates of genotype 3 (85.5 %) prevailed in the group of strains isolated from ixodid ticks, of which 29.6 % belonged to "Vasilchenko", and 25.6 % to "Zausaev"subgenotypes. Genotypes 1 and 2 were represented by single isolates (3.3 % and 1.3 %, respectively). Among strains isolated from warm-blooded hosts, the proportion of genotype 1 was 35.3 %, genotype 2 -11.8 %, genotype 3 - 35.3 %. More than half of genotype 3 strains (58.3 %) were related to "Vasilchenko" subgenotype, and the strains of "Zausaev" subgenotype were not detected. The obtained data suggest that ixodid ticks and warm-blooded animals are amplifiers of TBEV of genotypes 3 and 1, respectively. The certain differences in the phenotypic characteristics of strains isolated from ticks and warm-blooded animals have been revealed. It has been found that strains isolated from ticks are more homogeneous in their antigenic characteristics, less heterogeneous in S-feature than strains isolated from warm-blooded sources. The strains isolated from warm-blooded animals demonstrate somewhat greater resistance to warming but reducing the reproduction of the virus at 42 Β°C, and have greater virulence compared to strains isolated from ticks
The distribution of tick-borne encephalitis virus genotypes in different types of landscapes of Eastern Siberia
To find out what is the role of some environmental factors in the formation of a heterogeneous viral population, the analysis of the distribution of tick-borne encephalitis virus (TBEV) genotypes in different types of landscapes of Eastern Siberia has been carried out. In our study, it was shown that TBEV area in all investigated landscapes of Eastern Siberia is represented not only by TBEV of genotype 3, absolute dominance of which is noted in the studied regions, regardless the landscapes where the foci are located, but also by strains of other genotypes. The relationship between genotypes 1,3 and "polytypic" strains with a certain type of landscape and the degree of their development by man has not been established. The prevailing number of strains of TBEV genotype 5 is isolated from the material collected in the Transbaikalia, in the landscapes of the mountain taiga. They, as well as the strain of genotype 4, are found in the foci, where joint circulation of several TBEV genotypes is noted. The strains of TBEV genotype 2 were revealed in the landscapes of plains and plateaus taiga and insular steppes and forest steppes of the southern part of the Baikal region. Moreover, the detection of RNA of TBEV genotype 2 in the "polytipic" 763-87 and 765-87 strains isolated in the floodplain and forest-steppe landscapes of the Barguzin hollow does not exclude the possibility of circulation of TBEV genotype 2 in the Transbaikalian foci. The greatest genetic heterogeneity of TBEV is observed in natural foci located along Lake Baikal and in the southeastern part of Transbaikalia
APPLICATION OF MATHEMATICAL METHOD PREDICTIONS FOR IDENTIFICATION OF PATTERNS RELATIONS MUTATIONS IN PROTEINS ENCEPHALITIS VIRUS AND A MANIFESTATION OF ITS PHENOTYPIC TRAITS
We studied the natural connections between the amino acid sequences of proteins C, prM, E and NS1 virus strains of tick-borne encephalitis (TBE) and their three phenotypic traits -neuroinvasiveness, thermal stability and thermoresistance. Coupling strength is assessed using measures of competitive sequence similarity of each strain with reference strains. For such purposes subsets of strain sections are chosen amino acid composition specifics of which can predict the value of a phenotypic trait of interest. The possibility to predict missing elements in data both in amino acid composition, and in target properties is demonstrated. The relationships between pairs of phenotypic traits of strains were evaluated
SPECIES AND GENETIC VARIETY OF TICK INFECTIONS PATHOGENS ON THE TERRITORY OF THE EASTERN SIBERIA
The article sums up long-term, researches of studying of species and genetic variety of tick infections pathogens in combined, natural focuses of the Eastern Siberia. The results of the research testify to the high genetic variety of regional population, of tick-borne encephalitis virus that is represented, by the strains of Far-Eastern, West, Ural-Siberian genotypes, the strains of the group 886 and. 178-79. Infection of Ixodidae by the next pathogens: B. garinii, B. afzelii, R. sibirica, R. raoultii (Π³Π΅Π½ΠΎΡΠΈΠΏΡ. R. sp. DnS14, R. sp. DnS28), E. muris, A. phagocytophilum ΠΈ Candidatus Β«Neoehrlichia mikurensisΒ» was determined
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