Estudio etnobotánico de áreas del noroeste de la provincia de Isfahan, Iran

Iran is one of the countries which enormously used medicinal plants from the ancient times. In this study, we documented the ethnobotanical data of Golpayegan, and Shahin Shahr and Meymeh counties (Isfahan province) for the first time in Iran. Field study and plant collecting were done in different seasons in 2016–2017. Ethnobotanical data were analyzed using information of questionnaires gained from 27 inhabitants. Totally, 48 plants of 24 families were recorded from studied areas. Asteraceae (36%) and Lamiaceae (23%) are those containing the biggest numbers of cited plants. Moreover, leaves (45.83%) and seeds (18.75%) were the most used parts. This study indicated the importance of useful plants of studied areas and the necessity of doing this kind of research on other parts of country in order to preserve this valuable information of local inhabitants.Irán es un país que ha utilizado de manera destacada las plantas medicinales desde la antigüedad. En este estudio, documentamos los datos etnobotánicos de los condados de Golpayegan, Shahin Shahr y Meymeh (provincia de Isfahán) por primera vez en Irán. El estudio de campo y la recolección de plantas se realizaron en diferentes temporadas en 2016 y 2017. Los datos etnobotánicos se analizaron utilizando información proveniente de cuestionarios realizados a 27 informantes. En total, se registraron 48 plantas de 24 familias de las áreas estudiadas. Las familias Asteraceae (36%) y Lamiaceae (23%) son las que contienen un mayor número de plantas citadas. Las hojas (45,83%) y las semillas (18,75%) son las partes de planta más utilizadas. Este estudio indica la importancia de las plantas útiles de las áreas estudiadas y la necesidad de realizar este tipo de investigación en otras partes del país con el fin de preservar esta valiosa información de los habitantes locales

Species differentiation in annual Persicaria based on different markers

Persicaria with 70–100 species in the world is distributed in temperate regions of both hemispheres. It has 11 species in Iran growing in moist areas and margins of rivers. Through hybridisation, plasticity and existence of overlapping habitats, species identification shows difficulty. In this study, we aimed to investigate karyotype characters and morphological features, evaluate genetic variability within and among species studied and examine species relationship using ISSR data. Nine annual taxa of Persicaria were gathered from different localities in Iran and used for studies. Our studies showed that combination of karyological, morphological and molecular data can delimit species studied. Based on karyological results, three chromosome counts (P. hydropiper (2n = 2x = 20), P. maculosa (2n = 2x = 22), P. orientalis (2n = 2x = 22)) were the first reports for the Flora of Iran. Analyses of morphological characters showed diagnostic features among taxa. STRUCTURE and AMOVA analyses showed high intraspecific genetic diversity. Our results suggested that phenotypic plasticity and hybridisation may cause genetic diversity within Persicaria species

Avaliação de dois métodos para condicionamento e coleta de sêmen em quatro espécies do gênero Mazama

O desenvolvimento de técnicas não invasivas para a obtenção de sêmen de cervídeos facilita a criação de bancos genômicos, que são importantes instrumentos para a conservação ex situ e in situ. Este trabalho teve como objetivo criar uma metodologia não-invasiva de coleta de sêmen e comparar duas técnicas de coleta em quatro espécies do gênero Mazama: M. americana, M. gouazoubira, M. nana e M. nemorivaga. Para tanto, foram utilizados seis machos (M) e duas fêmeas (F) da espécie M. americana, 3M e 2F de M. gouazoubira, 1M e 1F de M. nana e 2M e 1F de M. nemorivaga. Para cada técnica testada, foi realizado um período de habituação dos animais ao manejo. Em seguida, duas técnicas de condicionamento e coleta foram avaliadas. Na primeira delas foi utilizada uma fêmea em estro com desvio lateral do pênis para vagina artificial (FEDL), obtendo-se a coleta de 50% dos indivíduos (100% dos machos de M. gouazoubira e 50% dos machos de M. americana), não obtendo ejaculados das demais espécies. Na segunda técnica, utilizando um manequim taxidermizado com urina de fêmea em estro (MUFE) não foi possível a coleta de nenhum ejaculado. Em todas as fases foi observado o comportamento do macho quanto ao tempo de interesse e aproximação, reflexo de "Flehmen", ato de cheirar ou lamber, exposição do pênis, ereção, número de falsas montas, tentativas de cópula e ocorrência de agressividade entre os animais

The physiological impact of high?intensity interval training in octogenarians with comorbidities

BackgroundDeclines in cardiorespiratory fitness (CRF) and fat-free mass (FFM) with age are linked to mortality, morbidity and poor quality of life. High-intensity interval training (HIIT) has been shown to improve CRF and FFM in many groups, but its efficacy in the very old, in whom comorbidities are present is undefined. We aimed to assess the efficacy of and physiological/metabolic responses to HIIT, in a cohort of octogenarians with comorbidities (e.g. hypertension and osteoarthritis).MethodsTwenty-eight volunteers (18 men, 10 women, 81.2 ± 0.6 years, 27.1 ± 0.6 kg·m−2) with American Society of Anaesthesiology (ASA) Grade 2–3 status each completed 4 weeks (12 sessions) HIIT after a control period of equal duration. Before and after each 4 week period, subjects underwent body composition assessments and cardiopulmonary exercise testing. Quadriceps muscle biopsies (m. vastus lateralis) were taken to quantify anabolic signalling, mitochondrial oxidative phosphorylation, and cumulative muscle protein synthesis (MPS) over 4-weeks.ResultsIn comorbid octogenarians, HIIT elicited improvements in CRF (anaerobic threshold: +1.2 ± 0.4 ml·kg−1·min−1, P = 0.001). HIIT also augmented total FFM (47.2 ± 1.4 to 47.6 ± 1.3 kg, P = 0.04), while decreasing total fat mass (24.8 ± 1.3 to 24 ± 1.2 kg, P = 0.0002) and body fat percentage (33.1 ± 1.5 to 32.1 ± 1.4%, P = 0.0008). Mechanistically, mitochondrial oxidative phosphorylation capacity increased after HIIT (i.e. citrate synthase activity: 52.4 ± 4 to 67.9 ± 5.1 nmol·min−1·mg−1, P = 0.005; membrane protein complexes (C): C-II, 1.4-fold increase, P = 0.002; C-III, 1.2-fold increase, P = 0.03), as did rates of MPS (1.3 ± 0.1 to 1.5 ± 0.1%·day−1, P = 0.03). The increase in MPS was supported by up-regulated phosphorylation of anabolic signalling proteins (e.g. AKT, p70S6K, and 4E-BP1; all P < 0.05). There were no changes in any of these parameters during the control period. No adverse events were reported throughout the study.ConclusionsThe HIIT enhances skeletal muscle mass and CRF in octogenarians with disease, with up-regulation of MPS and mitochondrial capacity likely underlying these improvements. HIIT can be safely delivered to octogenarians with disease and is an effective, time-efficient intervention to improve muscle mass and physical function in a short time frame

A survey on the prevalence of teat end hyperkeratosis in dairy farms of Tabriz

One of the most important defense mechanisms of the bovine mammary gland is teat sphincter and it’s keratinized and stratified squamous epithelium, which extends through the teat sphincter and after every milking secrets keratin and closes the capillary canal of the teat sphincter by keratin plaque. Over secretion of keratin due to repeated stimulations of teat end such as inappropriate performance of milking machine results in hyperkeratosis and finally leads to mastitis, black spot and increase in somatic cell counts. In this study 4 herds from dairy farms around Tabriz including 860 dairy cows were studied. Two herds were milked twice a day and the other herds were milked three times a day. At the time of milking the teat ends were investigated the presence of hyperkeratosis and graded into grades one to four. The results of this study indicated that the prevalence of teat end hyperkeratosis in herds which were milked three times a day (14/1%) was significantly more than the herds which were milked twice a day(%12/3) (

Study on the relationship between milk urea nitrogen (MUN) and fertility in dairy cattle houses in Tabriz

The aim of this study was to assess the relationship between milk urea nitrogen (MUN) level and reproductive performance in high yielding dairy cattle houses in Tabriz, Iran. Among 213 selected dairy cattle, 76 heads (35.7%) have MUN 16 mg/dl (mean = 17.46 mg/dl). Our results indicated that MUN level in 81 heads of dairy cattle (total 124 heads) with mastitis, dystocia, laminitis, uterine infections or placenta replacement was higher than 16 mg/dl. We only observed a significantly positive association between MUN levels and dystocia (p= 0.032), while the association between MUN levels and incidence of other diseases was not statistically significant. The results of this study indicated that MUN level significantly influences the reproductive parameters including days open, calving to first service, first service conception risk, and number of services per conception (

Prevalence of antibodies to BVD virus in milk tanks, dairy farms in Tabriz with history of abortions in 90-1389

Bovine viral diarrhea (BVD) is the most important bovine viral disease across the world. The virus belongs to Flavi viridae and of Pesti virus genus which causes to various syndromes. The virus affects the reproduction, respiratory, digestive, cardiovascular, immunity, lymphatic, muscular  and central nervous systems .Today, different tests (virus isolation, RT-PCR, capturing ELISA, direct and indirect ELISA  and immune fluorescence test) have be designed in order to diagnosis viral infection of cattle’s viral diarrhea. This study aimed at demonstrating the importance of the disease in cattle’s abortion of Tabriz suburb’s cattle pens in 89-90 by measurement antibody titre of produced milk. ELISA test was used in the present study since ELISA test of bulk milk is one of the diagnostic methods in cattle pens according to the regulations of counyry veterinarian organization for controlling BVD-MD disease. The results demonstrated that there were 9 positive and 11 negative samples of 20 milk samples. The results suggest that of the rate of milk tanks contamination of Tabriz suburb is about 45% in 89-90 which shows relatively 2% resistant contamination in cattle pens of the region