Particularităţile morfo-culturale ale tulpinii Aspergillus niger 33 CNMN FD 06A - producător de amilaze

Scopul cercetărilor a constat în studierea particularităţilor morfologice – creşterea, sporularea, dimensiunile, forma, aspectul şi culoarea coloniilor ale tulpinii în studiu la cultivarea pe medii nutritive agarizate

The influence of some metal nanooxides on the exocellular amylase activity of Aspergillus niger CNMN FD 06 mycelial fungal strain

In the research were included the nanoparticles (NPs) as follow: nanodioxide of Ti (TiO2) with dimensions of 21 nm and <100 nm, titanium silicon oxide nanoparticles TiSiO4<50 nm, nanooxides Fe3O4 50-100 nm, ZnO ≤50 nm and CuO <50 nm, as well as Cu metal (99,5%) with sizes of < 60-80 nm. The amylolytic activity of the strain Aspergillus niger CNMN FD 06 was monitored during the 5-7th days of cultivation – a period that corresponds to the maximum manifestation of amylase activity in the producer under submerged cultivation in classic conditions (control, without NPs). The nanocomposites were included in the culture medium in concentrations of 5 mg/L, 10 mg/L and 15 mg/L

Aspects of directed synthesis of proteases in mycelial fungi using metal nano-oxides

Data on the effect of some metal nano-oxides on the biosynthesis of individual components of proteolytic enzymes complex of micromycetes Trichoderma koningii CNMN FD 15 and Fusarium gibbosum CNMN FD 12 have been described. For each strain, optimum conditions of cultivation in the presence of the studied nanoparticles, which provide an increased level of proteolytic activity, have been determined. It has been emphasized that the stimulating effect depends on the composition, size, and concentration of the nanomaterials and on the initial pH value of the medium