43 research outputs found
Hippocampal Proteomic and Metabonomic Abnormalities in Neurotransmission, Oxidative Stress, and Apoptotic Pathways in a Chronic Phencyclidine Rat Model
Full text link
Calculation of the adsorption isotherm from solution on a nonporous adsorbent according to the adsorption isotherms of the components from the gas phase
No full textAdsorption of mixtures of benzene and trimethylpentane vapors on graphitized carbon black
No full text
A new method of calculation of the adsorption of binary mixtures of vapors on microporous adsorbents
No full textAdsorption of a gas mixture Communication 7. Joint adsorption of a binary mixture of vapors on activated charcoal
No full textNascent RNA antagonizes the interaction of a set of regulatory proteins with chromatin.
No full textA number of regulatory factors are recruited to chromatin by specialized RNAs. Whether RNA has a more general role in regulating the interaction of proteins with chromatin has not been determined. We used proteomics methods to measure the global impact of nascent RNA on chromatin in embryonic stem cells. Surprisingly, we found that nascent RNA primarily antagonized the interaction of chromatin modifiers and transcriptional regulators with chromatin. Transcriptional inhibition and RNA degradation induced recruitment of a set of transcriptional regulators, chromatin modifiers, nucleosome remodelers, and regulators of higher-order structure. RNA directly bound to factors, including BAF, NuRD, EHMT1, and INO80 and inhibited their interaction with nucleosomes. The transcriptional elongation factor P-TEFb directly bound pre-mRNA, and its recruitment to chromatin upon Pol II inhibition was regulated by the 7SK ribonucleoprotein complex. We postulate that by antagonizing the interaction of regulatory proteins with chromatin, nascent RNA links transcriptional output with chromatin composition
