Characterization of the myosin light-chain-2 gene of Drosophila melanogaster.

Recombinant DNA clones encoding the Drosophila melanogaster homolog of the vertebrate myosin light-chain-2 (MLC-2) gene have been isolated. This single-copy gene maps to the chromosomal locus 99E. The nucleotide sequence was determined for a 3.4-kilobase genomic fragment containing the gene and for two MLC-2 cDNA clones generated from late pupal mRNA. Comparison of these sequences shows that the gene contains two introns, the positions of which are conserved in the corresponding rat sequence. Extension of a primer homologous to the mRNA reveals two start sites for transcription 12 nucleotides apart. The sequence TATA is not present ahead of the mRNA cap site. There are two major sites of poly(A) addition separated by 356 nucleotides. The protein sequence derived from translation of the cDNA sequence shows a high degree of homology with that for the DTNB myosin light chain (MLC-2) of chicken. A lower degree of sequence homology was seen in comparisons with other evolutionarily related calcium-binding proteins. RNA blots show high levels of expression of several transcripts during the developmental time stages when muscle is being produced. In vitro translation of hybrid-selected RNA produces two polypeptides which comigrate on two-dimensional gels with proteins from Drosophila actomyosin, although the cDNA sequence reveals only one 26-kilodalton primary translation product

Drosophila melanogaster has only one myosin alkali light-chain gene which encodes a protein with considerable amino acid sequence homology to chicken myosin alkali light chains.

A chimeric lambda DNA molecule containing the myosin alkali light-chain gene of Drosophila melanogaster was isolated. The encoded amino acid sequence was determined from the nucleic acid sequence of a cDNA homologous to the genomic clone. The identity of the encoded protein was established by two criteria: (i) sequence homology with the chicken alkali light-chain proteins and (ii) comparison of the two-dimensional gel electrophoretic pattern of the peptides synthesized by in vitro translation of hybrid-selected RNA to that of myosin alkali light-chain peptides extracted from Drosophila myofibrils. There is only one myosin alkali light-chain in D. melanogaster; its chromosomal location is region 98B . This gene is abundantly expressed during the development of larval as well as adult muscles. The Drosophila protein appears to contain one putative divalent cation-binding domain (an EF hand) as compared with the three EF hands present in chicken alkali light chains

Darstellung des Konzeptes - DMA Decentralised Market Agent - zur Bewältigung zukünftiger Herausforderungen in Verteilnetzen

In der vorliegenden Veröffentlichung wird ein Konzept für einen neuen Marktakteur im Strommarkt vorgestellt, der im zukünftigen Smart Grid als Aggregator und Planer fungieren kann. Dieser Decentralised Market Agent - DMA - soll die Informationen aller vorhandenen Erzeugungsund Speicheranlagen, Lasten und Netzinformationen auf Verteilnetzebene aggregieren sowie mit lokalen Akteuren und an den zentralen Märkten agieren um einen kostenoptimalen Betrieb und Ausbau des Systems Verteilnetzes zu realisieren. Zur Handlungsfähigkeit dieser neuen Marktrolle bedarf es hochauflösender Messungen im Verteilnetz und einer \?real-time“ Aufbereitung der Messdaten. Im vorliegenden Paper sollen das Konzept sowie die notwendigen Bausteine zur Erreichung der Handlungsfähigkeit des DMA vorgestellt sowie die zukünftig geplanten Untersuchungen erläutert werden. Die detaillierte Entwicklung des Konzepts sowie weiterführende Analysen sind Teil des Projektes NEMAR - Netzbewirtschaftung als neue Marktrolle, gefördert durch BMWi im Rahmen der Forschungsinitiative Zukunftsfähige Stromnetze