Unravelling technology-acceptance factors influencing farmer use of banana tissue culture planting materials in Central Uganda

Effective management of plant health is fundamental for food and income security to meet the growing demands of local and global markets. This however requires farmers’ adequate access to quality planting materials under the prevailing contextual and psycho-social factors. This study, anchored in the Unified Theory of Acceptance and Use of Technologies, unravels technology-acceptance factors that influence farmers’ intentions to use banana tissue culture planting materials in the control of Banana Xanthomonas Wilt. Data were collected from 248 randomly sampled banana farmers using a structured questionnaire and analyzed using structural equation modelling to examine hypothesized paths in the uptake of banana tissue culture planting materials. Results show that farmer intentions to use tissue culture planting materials are dependent on two constructs: social influence and farmer innovativeness. However, social influence is the main predictor of intentions to use tissue culture planting materials. In particular, farmer innovativeness mediates facilitating conditions and social influence in predicting intentions to use tissue culture planting materials. Thus, this study reveals two factors that influence farmer intentions to use tissue culture planting materials: social influence and farmer innovativeness. The findings imply that social influence and farmer innovativeness are critical in disseminating novel agricultural technologies in Uganda and elsewhere

First reported case of fatal tuberculosis in a wild African elephant with past human-wildlife contact

Tuberculosis is emerging/re-emerging in captive elephant populations, where it causes morbidity and deaths, although no case of TB in wild African elephants has been reported. In this paper we report the first case of fatal TB in an African elephant in the wild. The infection with Mycobacterium tuberculosis was confirmed by post-mortem and histological examinations of a female sub-adult elephant aged >12 years that died in Tsavo East National Park, Kenya, while under treatment. This case is unique in that during its lifetime the elephant had contact with both humans and wild elephants. The source of the infection was unclear because the elephant could have acquired the infection in the orphanage or in the wild. However, our results show that wild elephants can maintain human TB in the wild and that the infection can be fata

Corrigendum: Three spatially separate records confirm the presence of and provide a range extension for the giant pangolin Smutsia gigantea in Kenya (Oryx (2022) DOI: 10.1017/S0030605322000126)

In the original publication of this article, the list of authors was incomplete, with only the following authors listed: Tommy Sandri, Claire Okell, Stuart Nixon, Naomi Matthews, Fred Omengo, James Mathenge, Stephen Ndambuki, Daniel W.S. Challender and Bradley Cain. The correct author list is: Tommy Sandri, Claire Okell, Stuart Nixon, Naomi Matthews, Fred Omengo, James Mathenge, Stephen Ndambuki, Daniel W.S. Challender, Richard Chepkwony, Patrick Omondi, Shadrack Ngene and Bradley Cain The article has been updated to include the correct author details and add the ORCID iD of Shadrack Ngene

User guide to the toolbox for working with root, tuber and banana seed systems. RTB User guide

This user guide to the Toolbox for working with root, tuber and banana seed systems introduces tools to diagnose, evaluate, and improve seed systems of banana, cassava, potato, sweetpotato, and yam. As a whole, these crops are called roots, tubers and bananas, and they are crucial for food security and income generation, especially in developing countries. All of these crops are reproduced vegetatively, from roots, tubers, stems, suckers or vines. This bulky planting material is expensive to transport. Vegetative seed is perishable and (except for potatoes, yams and a few other exceptions) must be planted as fresh as possible, and it is more likely to carry pests and diseases than true seed. Besides these unique challenges, improved seed systems of root, tuber and banana crops give farmers the opportunity to boost their livelihoods by accessing better quality planting material from landraces or improved varieties that are high yielding, resistant to stresses, more nutritious or more responsive to consumer demand

Rapid and high throughput molecular identification of diverse mosquito species by igh resolution melting analysis

Mosquitoes are a diverse group of invertebrates, with members that are among the most important vectors of diseases. The correct identification of mosquitoes is paramount to the control of the diseases that they transmit. However, morphological techniques depend on the quality of the specimen and often unavailable taxonomic expertise, which may still not be able to distinguish mosquitoes among species complexes (sibling and cryptic species). High resolution melting (HRM) analyses, a closed-tube, post-polymerase chain reaction (PCR) method used to identify variations in nucleic acid sequences, has been used to differentiate species within the Anopheles gambiae and Culex pipiens complexes. We validated the use of PCR-HRM analyses to differentiate species within Anopheles and within each of six genera of culicine mosquitoes, comparing primers targeting cytochrome b (cyt b), NADH dehydrogenase subunit 1 (ND1), intergenic spacer region (IGS) and cytochrome c oxidase subunit 1 (COI) gene regions. HRM analyses of amplicons from all the six primer pairs successfully differentiated two or more mosquito species within one or more genera (Aedes (Ae. vittatus from Ae. metallicus), Culex (Cx. tenagius from Cx. antennatus, Cx. neavei from Cx. duttoni, cryptic Cx. pipiens species), Anopheles (An. gambiae s.s. from An. arabiensis) and Mansonia (Ma. africana from Ma. uniformis)) based on their HRM profiles. However, PCR-HRM could not distinguish between species within Aedeomyia (Ad. africana and Ad. furfurea), Mimomyia (Mi. hispida and Mi. splendens) and Coquillettidia (Cq. aurites, Cq. chrysosoma, Cq. fuscopennata, Cq. metallica, Cq. microannulatus, Cq. pseudoconopas and Cq. versicolor) genera using any of the primers. The IGS and COI barcode region primers gave the best and most definitive separation of mosquito species among anopheline and culicine mosquito genera, respectively, while the other markers may serve to confirm identifications of closely related sub-species. This approach can be employed for rapid identification of mosquitoes

Linking soil microbial community structure to potential carbon mineralization: A continental scale assessment of reduced tillage

Potential carbon mineralization (Cmin) is a commonly used indicator of soil health, with greater Cmin values interpreted as healthier soil. While Cmin values are typically greater in agricultural soils managed with minimal physical disturbance, the mechanisms driving the increases remain poorly understood. This study assessed bacterial and archaeal community structure and potential microbial drivers of Cmin in soils maintained under various degrees of physical disturbance. Potential carbon mineralization, 16S rRNA sequences, and soil characterization data were collected as part of the North American Project to Evaluate Soil Health Measurements (NAPESHM). Results showed that type of cropping system, intensity of physical disturbance, and soil pH influenced microbial sensitivity to physical disturbance. Furthermore, 28% of amplicon sequence variants (ASVs), which were important in modeling Cmin, were enriched under soils managed with minimal physical disturbance. Sequences identified as enriched under minimal disturbance and important for modeling Cmin, were linked to organisms which could produce extracellular polymeric substances and contained metabolic strategies suited for tolerating environmental stressors. Understanding how physical disturbance shapes microbial communities across climates and inherent soil properties and drives changes in Cmin provides the context necessary to evaluate management impacts on standardized measures of soil microbial activity