Combined functional and immunochemical analysis ofnormal and abnormal human factor

A B S T R A C T Human Factor X was isolated from Cohn fraction III and characterized by polyacrylamide gel electrophoresis, amino acid composition, and isoelectric focusing. Two molecular forms with biological activity were observed at isoelectric points of 4.8 and 5.0. Antisera generated to Factor X was monospecific and used to establish an equilibrium competitive inhibition radioimmunoassay. This assay was specific for human Factor X and did not cross-react with human prothrombin or bovine Factor X within the sensitivity range of 6-300 ng Factor X antigen/ml. The mean concentration of Factor X based on the antigen was 11.9 ,ug0ml, whereas concentration values based on coagulant activity was 7.8 ,ug/ml. This 30% difference in measurement appears to result from the presence of a subpopulation of Factor X molecules devoid of coagulant activity. The radioimmunoassay was used to qualitatively and quantitatively compare purified Factor X to plasmic Factor X obtained from normal, warfarintreated, acquired Factor X-deficient, and congenitaldeficient patients. In all but one case, the Factor X present in these plasmas was immunochemically identical to the purified Factor X and permitted precise quantitation of these abnormal Factor X molecules. Factor X procoagulant activity was analyzed relative to Factor X antigen and the specific activities were used to characterize normal and abnormal Factor X molecules. Reduced Factor X activity in plasmas from warfarin-treated and acquired Factor Xdeficient patients was attributed to both decreases in Factor X antigen and decreased function of the Factor X molecules. Congenitally deficient patients, in general, showed a reduction in Factor X antigen in parallel with Factor X procoagulant activities This is publicatio

A Search for Cosmic Microwave Background Anisotropies on Arcminute Scales with Bolocam

We have surveyed two science fields totaling one square degree with Bolocam at 2.1 mm to search for secondary CMB anisotropies caused by the Sunyaev- Zel'dovich effect (SZE). The fields are in the Lynx and Subaru/XMM SDS1 fields. Our survey is sensitive to angular scales with an effective angular multipole of l_eff = 5700 with FWHM_l = 2800 and has an angular resolution of 60 arcseconds FWHM. Our data provide no evidence for anisotropy. We are able to constrain the level of total astronomical anisotropy, modeled as a flat bandpower in C_l, with frequentist 68%, 90%, and 95% CL upper limits of 590, 760, and 830 uKCMB^2. We statistically subtract the known contribution from primary CMB anisotropy, including cosmic variance, to obtain constraints on the SZE anisotropy contribution. Now including flux calibration uncertainty, our frequentist 68%, 90% and 95% CL upper limits on a flat bandpower in C_l are 690, 960, and 1000 uKCMB^2. When we instead employ the analytic spectrum suggested by Komatsu and Seljak (2002), and account for the non-Gaussianity of the SZE anisotropy signal, we obtain upper limits on the average amplitude of their spectrum weighted by our transfer function of 790, 1060, and 1080 uKCMB^2. We obtain a 90% CL upper limit on sigma8, which normalizes the power spectrum of density fluctuations, of 1.57. These are the first constraints on anisotropy and sigma8 from survey data at these angular scales at frequencies near 150 GHz.Comment: 68 pages, 17 figures, 2 tables, accepted for publication in Ap

A Fluctuation Analysis of the Bolocam 1.1mm Lockman Hole Survey

We perform a fluctuation analysis of the 1.1mm Bolocam Lockman Hole Survey, which covers 324 square arcmin to a very uniform point source-filtered RMS noise level of 1.4 mJy/beam. The fluctuation analysis has the significant advantage of utilizing all of the available data. We constrain the number counts in the 1-10 mJy range, and derive significantly tighter constraints than in previous work: the power-law index is 2.7 (+0.18, -0.15), while the amplitude is equal to 1595 (+85,-238) sources per mJy per square degree, or N(>1 mJy) = 940 (+50,-140) sources/square degree (95% confidence). Our results agree extremely well with those derived from the extracted source number counts by Laurent et al (2005). Our derived normalization is about 2.5 times smaller than determined by MAMBO at 1.2mm by Greve et al (2004). However, the uncertainty in the normalization for both data sets is dominated by the systematic (i.e., absolute flux calibration) rather than statistical errors; within these uncertainties, our results are in agreement. We estimate that about 7% of the 1.1mm background has been resolved at 1 mJy.Comment: To appear in the Astrophysical Journal; 22 pages, 9 figure

Spherical harmonic decomposition applied to spatial-temporal analysis of human high-density EEG

We demonstrate an application of spherical harmonic decomposition to analysis of the human electroencephalogram (EEG). We implement two methods and discuss issues specific to analysis of hemispherical, irregularly sampled data. Performance of the methods and spatial sampling requirements are quantified using simulated data. The analysis is applied to experimental EEG data, confirming earlier reports of an approximate frequency-wavenumber relationship in some bands.Comment: 12 pages, 8 figures, submitted to Phys. Rev. E, uses APS RevTeX style

Studies of atmospheric noise on Mauna Kea at 143 GHz with Bolocam

We report measurements of the fluctuations in atmospheric emission (atmospheric noise) above Mauna Kea recorded with Bolocam at 143 GHz. These data were collected in November and December of 2003 with Bolocam mounted on the Caltech Submillimeter Observatory (CSO), and span approximately 40 nights. Below ≃ 0.5 Hz, the data time-streams are dominated by the f-δ atmospheric noise in all observing conditions. We were able to successfully model the atmospheric fluctuations using a Kolmogorov-Taylor turbulence model for a thin wind-driven screen in approximately half of our data. Based on this modeling, we developed several algorithms to remove the atmospheric noise, and the best results were achieved when we described the fluctuations using a low-order polynomial in detector position over the 8 arcminute focal plane. However, even with these algorithms, we were not able to reach photon-background-limited instrument photometer (BLIP) performance at frequencies below ≃ 0.5 Hz in any observing conditions. Therefore, we conclude that BLIP performance is not possible from the CSO below ≃ 0.5 Hz for broadband 150 GHz receivers with subtraction of a spatial atmospheric template on scales of several arcminutes

The type and frequency of interactions that occur between staff and children outside in Early Years Foundation Stage settings during a fixed playtime period when there are tricycles available

This study reports on an investigation into adult and child interactions observed in the outdoor play environment in four Local Authority early years foundation stage settings in England. In this instance the common two features across the settings were the presence of tricycles and a timetabled outdoor play period. In total, across the four schools, there were 204 children. The study aimed to gain an understanding of the nature of the dialogues between staff and children, that is, the types of exchange that occurred when either the child approached an adult or the adult approached a child. The most frequent type of utterance was also analysed. The study concludes that adults in these settings spoke more than children and the greatest type of utterance was that of the adult about domestic matters. When the child initiated the conversation there were more extended child utterances than domestic utterances. This may suggest that children wish to be involved in conversations of depth and meaning and that staff need to become aware of how to develop this conversational language with children

The Bolocam Lockman Hole Millimeter-Wave Galaxy Survey: Galaxy Candidates and Number Counts

We present results of a new deep 1.1 mm survey using Bolocam, a millimeter-wavelength bolometer array camera designed for mapping large fields at fast scan rates, without chopping. A map, galaxy candidate list, and derived number counts are presented. This survey encompasses 324 arcmin^2 to an rms noise level (filtered for point sources) of 1.4 mJy/beam and includes the entire regions surveyed by the published 8 mJy 850 micron JCMT SCUBA and 1.2 mm IRAM MAMBO surveys. We reduced the data using a custom software pipeline to remove correlated sky and instrument noise via a principal component analysis. Extensive simulations and jackknife tests were performed to confirm the robustness of our source candidates and estimate the effects of false detections, bias, and completeness. In total, 17 source candidates were detected at a significance > 3.0 sigma, with six expected false detections. Nine candidates are new detections, while eight candidates have coincident SCUBA 850 micron and/or MAMBO 1.2 mm detections. From our observed number counts, we estimate the underlying differential number count distribution of submillimeter galaxies and find it to be in general agreement with previous surveys. Modeling the spectral energy distributions of these submillimeter galaxies after observations of dusty nearby galaxies suggests extreme luminosities of L = 1.0-1.6 x 10^13 L_solar and, if powered by star formation, star formation rates of 500-800 M_solar/yr.Comment: In press (to appear in Astrophysical Journal: 1 May 2005, v624, 1 issue); 21 pages, 15 figures, 3 table