ANATOMICAL AND CHEMICAL INVESTIGATIONS ON ASPARAGUS OFFICINALIS L. (ASPARAGACEAE)

Although Asparagus (Asparagus officinalis L.) is chiefly known as a vegetable herb, little information about the botanical characteristics of such plant are available. Therefore, it is aimed in this study to bring light more information about the morphological, anatomical and some important chemical contents of vegetative and reproductive organs of the plant, throughout the consecutive stages of its entire life span under the local conditions. Seeds of Asparagus (cv. Mary Washington 500 W) were sown 2nd Feb. 2013. The field experiment was carried out at the Experimental and Research Station of Fac. of Agric., Cairo Univ., Giza, Egypt during the two successive growing seasons 2013 and 2014. Asparagus is a herbaceous, perennial plant that grows up to 1.5- 2 m height. The plant posses aerial stems (ferns) and much- branched feathery foliage. The leaves are triangular scales like, 3-7 mm in length. The cladodes (modified stems) arise in the axis of scale leaves . Cladodes are found in fascicles (3-6) on each node. The flowers are bell- shaped, greenish –white to yellowish, 4.5-6.5mm long, with 6- tepals,single or in clusters of 2-3. Anatomical studies were carried out for various organs of Asparagus plant including, apex of the aerial stem, visible internode below shoot apex, median portion of the aerial stem, the cladode , median portion of spear, scale - like leaf, rhizome, adventitious root. The major chemical metabolites contents of spear were determined

Synergistic Influence of <i>Arbuscular mycorrhizal</i> Fungi Inoculation with Nanoparticle Foliar Application Enhances Chili (<i>Capsicum annuum</i> L.) Antioxidant Enzymes, Anatomical Characteristics, and Productivity under Cold-Stress Conditions

In this study, we aimed to evaluate the effects of Arbuscular mycorrhiza fungus (AMF) inoculation, foliar application of zinc oxide and selenium nanoparticles (ZnO-NPs and Se-NPs), and their combined interactions on the growth and productivity of chili pepper under cold-stress conditions. Two field experiments were successfully conducted during the winter seasons of 2021 and 2022 in an experimental field at the Faculty of Agriculture, Cairo University, Giza, Egypt. The results showed that, under cold stress, the combination of AMF inoculation and ZnO-NPs + Se-NPs as a foliar spray increased the average fruit weight by 92.4% and 98.7%, and the number of fruits by 34.6% and 54.8 compared to control treatment in the 2021 and 2022 seasons, respectively. Additionally, the combination of AMF and a mixture of nanoparticles (ZnO-NPs + Se-NPs) significantly increased the total marketable yield by 95.8% and 94.7% compared to the control, which recorded values of 2.4 and 1.9 kg m−2 in the 2021 and 2022 seasons, respectively. Furthermore, the combination of AMF and a mixture of nanoparticles (ZnO-NPs + Se-NPs) showed the highest total content of ascorbic acid and capsaicin in chili fruits compared to the other treatments. The combination of AMF and a mixture of nanoparticles (ZnO-NPs + Se-NPs) stimulated the accumulation of peroxidase (POD) and nitrogen glutamate dehydrogenase (GDH) while decreasing hydrogen peroxide (H2O2) and lipid peroxidation (MDA) contents. SDS analysis revealed that the application of ZnO-NPs, Se-NPs, AMF + ZnO-NPs, and AMF + ZnO-NPs + Se-NPs induced the emergence of new protein bands and reconstitution of those damaged by cold stress. Regarding histological structure, the combination of AMF inoculation and ZnO-NPs + Se-NPs as a foliar spray showed an enhancement in the thickness of grana thylakoids and increased the number of chloroplasts. Intriguingly, the findings showed that AMF and a mixture of nanoparticles (ZnO-NPs + Se-NPs) could offer guidance for increasing plant development and productivity under cold-stress conditions