Motile Artificial Chromatophores: Light-Triggered Nanoparticles for Microdroplet Locomotion and Color Change

Gold nanoparticles coated with a poly(N-isopropylacrylamide) (pNIPAM) shell undergo reversible dis/assembly below and above the critical temperature of 32°C. Loading these particles into microdroplets at high density creates light-driven artificial chromatophores. Triggering the nanoparticle assembly gives dramatic color changes from nanoparticle localization at the base of the droplets, resembling zebrafish melanophores. These reversible chromatophore states can be switched by both bulk and optical heating, explored here in individual microdroplets and in large cm^2 areas of close-packed droplets. Illuminating chromatophores off-center with a tightly focused beam results in droplet locomotion via two separate mechanisms, Marangoni interfacial shear and gas bubble propulsion, depending on optical power.ER

Patterned Arrays of Supramolecular Microcapsules

Micropatterning of hydrogel has brought innovative outcomes in fundamental and applied material sciences. Previous approaches have mainly been dedicated to fabricate arrays of bulk hydrogel beads, which have inherent challenges including loading ability, scalability, specificity and versatility. Here we present a methodology to create hollow microcapsule arrays from sessile microdroplets. The difference in wettability between hydrophilic and hydrophobic surfaces enables self-partitioning of liquid into microdroplet arrays, serving as microreservoirs to load complementarily-functionalized host-guest polymers, cucurbit[8]uril-threaded highly-branched polyrotaxanes (HBP-CB[8]) and naphthyl-functionalized hydroxyethyl cellulose (HEC-Np). The interfacial dynamic complexation between positively-charged HBP-CB[8] and HECNp occurred in the presence of negatively-charged surfactants, resulting in condensed supramolecular hydrogel skins. The hydrogel microcapsules were uniform in size, and were developed to encapsulate target cargos in a robust and well-defined manner. Moreover, the microcapsule substrates were further used for surface enhanced Raman spectroscopy (SERS) sensing upon loading of gold nanoparticles. This facile assembly of microcapsule arrays has potential applications in controlled cargo delivery, bio-sensing, high throughput analysis and sorting

The AMIGA sample of isolated galaxies. V. Quantification of the isolation

The AMIGA project aims to build a well defined and statistically significant reference sample of isolated galaxies in order to estimate the environmental effects on the formation and evolution of galaxies. The goal of this paper is to provide a measure of the environment of the isolated galaxies in the AMIGA sample, quantifying the influence of the candidate neighbours identified in our previous work and their potential effects on the evolution of the primary galaxies. Here we provide a quantification of the isolation degree of the galaxies in this sample. Our starting sample is the Catalogue of Isolated Galaxies (CIG). We used two parameters to estimate the influence exerted by the neighbour galaxies on the CIG galaxy: the local number density of neighbour galaxies and the tidal strength affecting the CIG galaxy. We show that both parameters together provide a comprehensive picture of the environment. For comparison, those parameters have also been derived for galaxies in denser environments such as triplets, groups and clusters. The CIG galaxies show a continuous spectrum of isolation, as quantified by the two parameters, from very isolated to interacting. The fraction of CIG galaxies whose properties are expected to be influenced by the environment is however low (159 out of 950 galaxies). The isolated parameters derived for the comparsion samples gave higher values than for the CIG and we found clear differences for the average values of the 4 samples considered, proving the sensitivity of these parameters. The environment of the galaxies in the CIG has been characterised, using two complementary parameters quantifying the isolation degree, the local number density of the neighbour galaxies and the tidal forces affecting the isolated galaxies. (Abridged)Comment: 10 pages, 12 figures, proposed for acceptance A&

Hydrophilic PDMS microchannels for high-throughput formation of oil-in-water microdroplets and water-in-oil-in-water double emulsions

Here we present a novel surface modification method based on the sequential layer-by-layer deposition of polyelectrolytes yielding hydrophilic microchannels in PDMS-based microfluidic devices. The coatings are long-term stable and allow for the generation of monodisperse oil-in-water microdroplets even several months after the channel surface treatment. Due to the robustness of the polyelectrolyte multilayers ultra-high flow rates can be applied, making high-throughput droplet formation in the jetting mode possible. Furthermore, we successfully used our method to selectively modify the surface properties in certain areas of assembled microchannels. The resulting partially hydrophilic, partially hydrophobic microfluidic devices allow for the production of monodisperse water-in-oil-in-water double emulsions.<br/

Microstructure and superconducting properties of hot isostatically pressed MgB2

Bulk samples of MgB2 have been formed by hot isostatic pressing (HIPping) of commercial powder at 100MPa and 950=B0C. The resulting material is 100% dense with a sharp superconducting transition at 37.5K. Microstructural studies have indicated the presence of small amounts of second phases within the material, namely MgO and B rich compositions, probably MgB4. Magnetisation measurements performed at 20K have revealed values of Jc=1.3 x 106A/cm2 at zero field, and 9.3 x 105A/cm2 at 1T. Magneto optical (MO) studies have shown direct evidence for the superconducting homogeneity and strong intergranular current flow in the material.Comment: 3 pages, 6 figures, text updated, new references included and discussed. Submitted to Superconductor Science and Technolog

Differentially Addressable Cavities within Metal-Organic Cage-Cross-Linked Polymeric Hydrogels

Here we report a new class of hydrogels formed by polymers that are cross-linked through subcomponent self-assembled metal–organic cages. Selective encapsulation of guest molecules within the cages creates two distinct internal phases within the hydrogel, which allows for contrasting release profiles of related molecules depending on their aptitude for encapsulation within the cages. The hydrogels were fabricated into microparticles via a droplet-based microfluidic approach and proved responsive to a variety of stimuli, including acid and competing amine or aldehyde subcomponents, allowing for the triggered release of cargo

Focused surface acoustic waves induced microdroplets generation and its application for microgels

We demonstrate a novel microdroplets generation method by using focused surface acoustic waves (FSAW). The method differs from previous work in the mechanism and the geometry structure of the chip, which is depend on FSAW rather than flow shearing. The acoustic radiation force arising from FSAW acts on the oil-water interface, breaking up the water into microdroplets whose size can be controlled by tuning the driving voltage and frequency of FSAW. This approach overcomes the limitation of microchannel structure and capillary number of the traditional microfluidic droplets formation methods, which enables a fully electrical control of microdroplets size, with a good uniformity. Further, the FSAW-induced microdroplets are used as templates to prepare microgels with uniform size, offering a new platform for the design and synthesis of monodispersed functional microspheres.This project is financially supported by the National Natural Science Foundation of China (51575439 and 51711530237), Royal Society-Newton Mobility Grant (IE161541), and 111 project (B12016)

Star-forming galaxies in low-redshift clusters: Data and integrated galaxy properties

This paper is a continuation of an ongoing study of the evolutionary processes affecting cluster galaxies. Both CCD R band and H alpha narrow-band imaging was used to determine photometric parameters (m_(r), r_(24), H alpha flux and equivalent width) and derive star formation rates for 227 CGCG galaxies in 8 low-redshift clusters. The galaxy sample is a subset of CGCG galaxies in an objective prism survey of cluster galaxies for H alpha emission. It is found that detection of emission-line galaxies in the OPS is 85%, 70%, and 50% complete at the mean surface brightness values of 1.25 x 10^(-19), 5.19 x 10^(-20), and 1.76 x 10^(-20) W m^(-2) arcsec^(-2), respectively, measured within the R band isophote of 24 mag arcsec^(-2) for the galaxy. The CCD data, together with matched data from a recent H alpha galaxy survey of UGC galaxies within 3000 km s^(-1), will be used for a comparative study of R band and H alpha surface photometry between cluster and field spirals.Comment: Accepted for publication in A&A. 11 pages, including 6 figure

Droplet-based microfluidic screening and sorting of microalgal populations for strain engineering applications

The application of microfluidic technologies to microalgal research is particularly appealing since these approaches allow the precise control of the extracellular environment and offer a high-throughput approach to studying dynamic cellular processes. To expand the portfolio of applications, here we present a droplet-based microfluidic method for analysis and screening of Phaeodactylum tricornutum and Nannochloropsis gaditana, which can be integrated into a genetic transformation workflow. Following encapsulation of single cells in picolitre-sized droplets, fluorescence signals arising from each cell can be used to assess its phenotypic state. In this work, the chlorophyll fluorescence intensity of each cell was quantified and used to identify populations of P. tricornutum cells grown in different light conditions. Further, individual P. tricornutum or N. gaditana cells engineered to express green fluorescent protein were distinguished and sorted from wild-type cells. This has been exploited as a rapid screen for transformed cells within a population, bypassing a major bottleneck in algal transformation workflows and offering an alternative strategy for the identification of genetically modified strains